5 Written questions
5 Matching questions
- In 1997, Dolly the sheep was cloned. Which of the following processes was used?
A) fusion of an adult cell's nucleus with an enucleated sheep egg, followed by incubation in a
B) replication and dedifferentiation of adult stem cells from sheep bone marrow
C) separation of an early stage sheep blastula into separate cells, one of which was incubated in a surrogate ewe
D) isolation of stem cells from a lamb embryo and production of a zygote equivalent
E) use of mitochondrial DNA from adult female cells of another ewe
- A researcher introduces double-stranded RNA into a culture of mammalian cells, and can identify its location or that of its smaller subsections experimentally, using a fluorescent probe. Within the first quarter hour, the researcher sees that the intact RNA is found in the cells. After 3 hours, she is not surprised to find that
A) the double-stranded RNA binds to mRNAs to prevent translation.
B) the double-stranded RNA replicates itself.
C) the RNA is degraded by 5' and 3' exonucleases.
D) Dicer enzyme has reduced it to smaller double-stranded pieces.
E) the double-stranded RNA binds to tRNAs to prevent translation.
- Which of the following is characteristic of the lytic cycle?
A) The virus-host relationship usually lasts for generations.
B) Many bacterial cells containing viral DNA are produced.
C) Viral DNA is incorporated into the host genome.
D) The viral genome replicates without destroying the host.
E) A large number of phages are released at a time.
- Why are BACs preferred today rather than bacteriophages for making genomic libraries?
A) The BAC can carry entire genes and their regulatory elements.
B) The BAC carries more DNA.
C) Larger BACs are easier to store.
D) The BAC carries more DNA, the BAC can carry entire genes and their regulatory elements, and larger BACs are easier to store.
E) The BAC can carry entire genes and their regulatory elements, and larger BACs are easier to store.
- DNA microarrays have made a huge impact on genomic studies because they
A) dramatically enhance the efficiency of restriction enzymes.
B) can be used to eliminate the function of any gene in the genome. C) allow the expression of many or even all of the genes in the genome to be compared at
D) allow physical maps of the genome to be assembled in a very short time.
E) can be used to introduce entire genomes into bacterial cells.
- a E) a large number of phages are released at a time
- b A) fusion of an adult cell's nucleus with an enucleated sheep egg, followed by incubation in a surrogate
- c C) allow the expression of many or even all of the genes in the genome to be compared at once
- d D) The BAC carries more DNA, the BAC can carry entire genes and their regulatory elements, and larger BACs are easier to store.
- e D) Dicer enzyme has reduced it to smaller double-stranded pieces
5 Multiple choice questions
- D) antibodies against specific proteins not found in the host membranes
- C) The RNA is only translated into a single long polypeptide, which is then cleaved into shorter ones
- C) ~6,300
- C) increased concentrations of sugars such as arabinose in the cell
- B) The fragments must be separated by electrophoresis, the fragments must be treated with heat or chemicals to separate the strands of the double helix, and the probe must be hybridized with the fragment.
5 True/False questions
The first cloned cat, called Carbon Copy, was a calico, but she looked significantly different from her female parent. Why?
A) Fur color genes in cats are influenced by differential acetylation patterns.
B) The environment, as well as genetics, affects phenotypic variation.
C) The telomeres of the parent's chromosomes were shorter than those of an embryo.
D) Cloned animals have been found to have a higher frequency of transposon activation
E) X inactivation in the embryo is random and produces different patterns. → A) nothing; they self-assemble
Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme?
TTTT → B) GGCC
Antiviral drugs that have become useful are usually associated with which of the following properties?
A) removal of viral proteins
B) interference with viral replication
C) prevention of the host from becoming infected
D) removal of viral mRNAs
E) ability to remove all viruses from the infected host → B) interference with viral replication
For a particular microarray assay (DNA chip), cDNA has been made from the mRNAs of a dozen patients' breast tumor biopsies. The researchers will be looking for
A) a pattern shared among some or all of the samples that indicates gene expression differing from control samples.
B) a pattern of fluorescence that indicates which cells are overproliferating.
C) a group of cDNAs that match those in non-breast cancer control samples from the same
D) a group of cDNAs that act differently from those on the rest of the grid.
E) a particular gene that is amplified in all or most of the patient samples. → B) the amino acid acts as a corepressor
Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
A) restriction enzyme--analysis of RFLPs
B) reverse transcriptase--production of cDNA from mRNA
C) electrophoresis--separation of DNA fragments
D) DNA ligase--cutting DNA, creating sticky ends of restriction fragments
E) DNA polymerase--polymerase chain reaction to amplify sections of DNA → E) it is an activator for other genes