| Term | Definition |
|---|
| (PA) Pseudomonas Isolation Agar | Isolation of Psuedomonas/Quadrant Streak/Selective..... 1) growth on streaked line= Psudomonas + (2) No growth= Psuedomonas - |
| (BESC) BILE Esculin Agar | Isolation of Enterococcus/Fish Tail/Sel. and Diff.... (1) Black= enterococccus + (2) Not black = Entercoccus - |
| (BA) Blood Agar | Test for hemolytic activity/Quadrant Streak/Differential/ autoclaved,cooled,then blood added.... (1) No Growth Evident (2) clear zone around colony viewed from bottom= Beta hemolysis. (3) Green/Brown medium disscolorization viewed from top= Alpha hemolysis. (4) No color change in medium= Gamma hemolysis |
| (MAC) MaConkey Agar | Isolation of Gram - Enterics/Quadrant Streak/Selective and Differential.... (1) Purple= lactose fermenter (2) Colorless= non lactose fermenter (3) No Growth. |
| (GLU) Phenol Red Glucose Broth | Fermentative Ability/Dip and Sworl/ Biochemical Class/ pH > 7= red pH<7= yellow..... (1)RED=alkaline=K, yellow=acid=A (2) Gas bubble in Durham tube= +, no gas bubble = "-" |
| (MSA) Mannitol Salt Agar | Isolates Staphylococcus Species: identifies Staphylococcus aureus./Quadrant Streak/Selective and Differential. SA= 7.5% NaCl.. DA= MAnnitol ..pH= Phenol Red.... (1) Growth medium turns lemon-yellow= Staphylococcus aureus + (2) Growth medium is pink= Staphylococcus epidermidis + (3) No Growth= Staphylococcus - |
| Enterococcus faecalis | opputunist/in intestinal tract/ can cause bacteremia,cyctitis,VRE(Vancomycin Resistant Enterococci) |
| Pseudomonas aeruginosa | oppurtinisitc pathogen/fuity odor/ blue-green pigment/grows at 25* C. |
| Escherichia coli | vitamins K and B complex/ indicates fecal contamination/ 0157:H7= enterohemorrhagic |
| Proteus vulgaris | swarming colonies on plates/ oppurtunist in the urinary tract/ drug resistant/strains a problem/nosocomial infections/trimeyhopin and sulfa compunds. |
| Staphylococcus aureus | drug resitant. Methicillin resitant staphylococcus aureus/Vanocomycin resistant Staphylococcus aureas |
| Staphylococcus epidermidis | opputunist/found on skin/ produces a slime layer that enables them to become attached to hospital equipment. |
| Clostridium sporogens | anaerobic/intestinal water loss and hemorrhaging by destroying intestinal epithelial cells. |
| Micrococcus lutues | appear in yellow culture |
| Micrococcus roseus | appears in red culture |
| Micrococcus | gram + cooci found in pairs of clusters,nonpathogenic,grows well at 25*C |
| Serratia marcescens | coliform/soil and water/opportunist in AIDS/pneumonia alcohol/ at 25*C RED PIGMENTED.DNAase |
| Bacillus megaterium | large bacillus/cytological studies/produces casease/gives milk its white color |
| Bacillus subtilis | found in soil/ bacitaracin and laundry detergent enzymes(protease)/paper production(amylease)/ resistant to chemicals and heat. |
| (TSA) Trypticase Soy Agar | growth of wide range of bacteria,General/Varied innoculation/ |
| (TSB) Trypticase Soy Broth | growth of wide range of bacteria/General/Dip and Swirl. |
| (TGB) Thioglycolate Broth | growth of anerobic microbes/BIOCHEMICAL/Dip and Swirl. (1)growth in top of aerobic layer=obligate aerobe (2) rowth throughout aerobic and anaerobic layers= faculative anaerobe (3) growth in bottom of anaerobic layer+ obligate anaerobe |
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