lc biology mandatory experiments food test bio

About this set

Created by:

reckdun  on March 6, 2012

Subjects:

lc biology

Description:

lc bio unit 1 mandatory experiments

Classes:

fifth year biology class 2012-2013, first year sc 2012, leaving cert biology

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lc biology mandatory experiments food test bio

protein test add an equal volume of
10% sodium hydroxide and 1% copper sulphate
1/27
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protein test add an equal volume of 10% sodium hydroxide and 1% copper sulphate
Positive test for protein purple
negative test for protein light blue
Test for reducing sugar Benedict,s or Fehlings 1 and 2 and gently heat
Test for glucose (reducing sugar) Benedict's solution then place in boiling water for 10 minutes
positive test for glucose brick red
negative test for glucose blue
test for starch also add few drops of dilute iodine
positive test for starch blue black
negative test for starch red-yellow
control for protein, glucose and starch use an equal volume of distilled water as the sample
test for lipids use brown paper
positive result for lipids translucent grease spot
D.C.P.I.P di chloro phenol indo phenol used to test for vit c
positive test for vit c blue colour dissappears
negative result for vit c blue colour persists
emulsion test Test Confirms the presence of lipids, relies on the fact that lipids are soluble in ethanol and insoluble in water forming a cloudy suspension
Biuret test for proteins; biuret solution initially blue, mix with proteins will cause a change to violet. Example foods: Beef, dairy, fish, peanuts
will benedicts work for sucrose no it is not a reducinng sugar
which test requires heat reducing sugar test Benedicts or Fehlings
what solution makes a suitable control distilled water
what is the purpose of a control to make a comparison to prove whether the experiment was proven or not
food solution suitable for protein test milk or albumen egg white
food suitable to show the presence of fat olive oil or butter
food suitable to show the presence of starch potato cream cracker
food suitable to show the presence of vitamin C kiwi, orange, black currant
Benedict's solution doesn't work for sucrose why? it is not a reducing sugar

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