Microbiology Information to Know

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What MO is:
Catalase +
Modified Oxidase =
Furazolidone Disk S
Bactracin Disk R

Staphylococcus

What MO is:
Catalase +
Modified Oxidase +
Furazolidone Disk R
Bactracin Disk S

Micrococcus

What MO is
Catalase =
Modified Oxidase =
Furazolidone Disk S
Bactracin Disk R

Rothia (Stomatococcus)

What MO causes skin infections, food poisoning, scalded skin syndrome and toxic shock syndrome?

S. aureus

What staphylococcus causes UTI?

S. saprophyticus

What causes Aggressive endocarditis?

S. lugdunensis

What will identify S. aureus?

Coagulase +
Latex agglutination +
Grows on MSA: yellow colonies

What is associated with indwelling medical devices?

Cogaulase-Negative Staphylococci

What MO is
Coagulase =
Novobiocin R

S saprophyticus

What is sticky staph?

Rothia (stomatococcus)

What MO is:
Catalase +
Tube coagulase =
Ornithine decarboxylase +
PYR +

S. lugdunensis

What is the hemolysis for:
GAS
GBS
GPS C, F, G
GDS
Pneumo
Viridans

GAS and Gps C, F, G - Beta
GBS - Beta and gamma
GDS and Viridans - Alpha and gamma
Pneumo- Alpha

What MO is GAS and causes pharyngitis, scarlet fever, skin infections, rheumatic fever, glomerulonephritis, and streptococcal toxic shock syndrome?

S. pyogenes

What MO is GBS and causes neonatal sepsis and meningitis?

S. agalactiae

What causes pneumonia and meningitis?

S. pneumoniae

What causes endocarditis?

Viridans streptococci

What GDS that is bacteremia associated with colon cancer?

S. gallolyticus
S. bovis

What is:
A disk S
SXT R
PYR +

GAS (S. pyogenes)

What is:
Camp +
SXT R
Hippurate +

GBS (S. agalactiae)

What is:
Bile-Esculin +
Salt +
PYR +

Enterococci

What is:
Bile esculin +
Salt =

NonEnterococci Group D

What is
Optochin S
Bile soluble

S. pneumoniae

What is the hemlysis for:
Arcanobacterium
Bacillus anthracis
Bacillus Species
Corynebacterium
Listeria
Lactobacillus & Erysipelotrix

Beta
Gamma
Beta
Gamma
Beta
Gamma and Alpha

What is:
Gram Stain: chinese letters (pleomorphic rods)
Catalase +
Motility =

Corynebacterium

What is:
Motility + (25)
Catalase +
Bile esculin +
Umbrella pattern in semisolid media
tumbling in wet mounts
Esculin hydrolysis +
Hippurate/Camp +

Listeria monocytogeneus

What is:
Gram Stain: GP cocci
Hemylosis Beta or gamma
Camp/Hippurate+

GBS

What uses media
BAP, cystine-tellurite, Pai, Tinsdale and Loeffler.
Methylene bluestain: metachromatic granules in cells;
Elek test for toxin?

C. diphtheriae

What MO do you get when ingest contaminated food; cause variety of infections in neonates, pregnant women, and immunocompromised patients?

Listeria monocytogenes

What MO is:
Glucose +
Maltos =
Lactose =
Sucrose =
Nitrate =
DNAse =
Hydroxyprolylaminopeptidase +
gamma-Glutamyl-aminoeptidase =
Beta-Glactosidase =
Butyrate esterase =

N. gonorrhoeae

What MO is:
Glucose +
Maltos +
Lactose =
Sucrose =
Nitrate =
DNAse =
Hydroxyprolylaminopeptidase V
gamma-Glutamyl-aminoeptidase +
Beta-Glactosidase =
Butyrate esterase =

N. meningitidis

What MO is:
Glucose +
Maltos +
Lactose +
Sucrose =
Nitrate =
DNAse =
Hydroxyprolylaminopeptidase +
gamma-Glutamyl-aminoeptidase =
Beta-Glactosidase +
Butyrate esterase =

N. Lactamica

What MO is:
Glucose =
Maltos =
Lactose =
Sucrose =
Nitrate +
DNAse +
Hydroxyprolylaminopeptidase =
gamma-Glutamyl-aminoeptidase =
Beta-Glactosidase =
Butyrate esterase +

M. catarrhalis

What MO is:
X and V factor +
No hemolysis?

H. influenzae

What MO is:
V factor +
No hemolysis?

H. parainfluenzae

What MO is V and X factor and has hemolysis?

H. haemolyticus

What MO is V factor and has hemolysis?

H. parahaemolyticus

Which MO is X factor and may be a weak hemolysis?

H. ducreyi

Which haemophilus has a school of fish cell arrangement and has a chancroid or soft chancre?

H. ducreyi

Which haemophilus causes Meningitis, Epiglottitis, Otitis media?

H. influenzae

Which serogroup belongs to the following:
S. dysenteriae
S. flexneri
S. boydii
S. sonnei

A
B
C
D

What MO's are Voges Proskauer (VP) positive?

Klebsella
Enterobacter
Serratia

What MO's are Phenylalanine Positive?

Proteus
Providencia
Morganella

What MO's are VP and Phenylalanine Negative?

E. coli
shigella
Citrobacter
Salmonella

What MO's are lactose Positive?

E. coli
Klebsiella
Enterobacter
Citrobacter (V)

What MO's are H2S positive?

Proteus
Salmonella
Citrobacter (V)

What MO's are Lysine Positive?

Salmonella
E. Coli

What MO's will appear as follows on Media:
EMB Metallic green sheeen
MAC Red/Pink
SS Red/Pink
HE Yellow/orange
XLD Yellow

E. coli

What MO will appear as follows on Media:
EMB Black/blue
MAC Red/Pink
SS Red/Pink
HE yellow/orange
XLD Yellow

Klebsiella and Enterobacter
Klebsiella will have a mucoid appearance

What MO will appear as follows on Media:
EMB Colorless
MAC Colorless
SS Colorless
HE Green
XLD Red

Shigella

What MO will appear as follows on media:
EMB Colorless
MAC Colorless
SS Colorless w/ black center
HE Green w/ black center
XLD Red w/ black center

Salmonella

What MO's will be glucose Fermenter, lactose fermenter and KIA is A/A?

E. coli
Klebsiella
Enterobacter
some citrobacter

What MO's will be Glucose Fermenter, Nonlactose Fermenter with KIA as K/A (Red/Yellow)?

Shigella
S. marcescens
Providencia
Morganella
Y. enterocolitica
Some citrobacter

What MO's will be Glucose Nonfermenter and KIA as K/K (Red/Red)?

Pseudomonas
Acinetobacter

What MO will have a reaction on Lysine iron agar as K/K (purple)?

E. coli
Klebsiella
Serratia

What MO will have K/K with H2S on LIA?

Salmonella

What MO will have R/A on LIA?

Proteus

What MO will have a K/A reaction in LIA?

Shigella
Enterobactor

What MO will have a K/A with H2S in LIA?

Some citrobactor

What is the positive result for ONPG?

Yellow

What reaction does S. marcescens have in TSI?

A/A

What color is the VP positive reaction?

Red

What color is the Citrate positive reaction?

Blue

What color is the Phenylalanine deaminase positive reaction?

Green

In the OF test, what MO gives a Yellow in both closed and open tubes?

E. Coli

In the OF test, What MO gives a yellow in open tube and a green/blue in closed tube?

Pseadomonas

In the OF test, What MO gives a green/blue color in open and closed tube?

Acinetobactor

What MO can infect any body site in immunocompromised patients (CSF/Burn) and causes Swimmer's ear?

Pseudomonas aeruginosa

What MO may resemble Enterobacteriaceae and is oxidase and nitrate reduction =?

Acinetobacter

What MO is sensitive to SXT, oxidizes glucose and maltose?

Stenotrophomonas maltophilia

Flavobacterium?

Often indicates colonization or contamination
Yellow pigment
Most oxidase +
Myrodies odoratum colonies sweet, fruity oder

Moraxella

Usually opportunist
Growth on BA
May grow on MAC
Oxidase +
Will become rod when grown with penicillin

Burkholderia Cepacia

Cystic Fibrosis Pt Cepacia Syndrome (resp tract)
Grows on BCSA or OFPBL Yellow colonies
Weak Oxidase +

Burkholderia pseudomallei

Melioidosis (vietnamese timebomb
Wrinkled colonies
Bipolar staining
Oxidase +

What can grow on MAC, have a metallic sheen, usually beta, smells like grapes or corn taco, and can be mucoid when isolated from Cystic Fibrosis pat?

Pseudomonas aeruginosa

What has pyocyanin production, oxidase positive, growth in 42, oxidizes glucose, TSI: A/no change?

Pseudomonas aeruginosa

What vibrio needs NaCl?

V. alginolyticus
V. parahaemolyticus
V. vulmificus

Which MO do not need NaCl to grow?

V. Cholerae
Aeromonas
Plesiomonas

What causes rice water stool?

V. cholerae

What causes gastric ulcers?

Helicobacter pylori

How do you identify Helicobacter?

Serology
Rapid urease
oxidase
catalase

C. jejuni or C. coli

Usually intestinal
grows on Campy-BAP or skirrow
42 degrees
Cephalothin R
Nalidixic acid S
Hippurate +

C. Fetus

Bactermia in immunocompromised pt
Blood cultures
BAP or Choc
37 degrees
Cephalothin S
Nalidixic Acid R

What motility does Campylobacter have?

Darts

What dz does Legionella cause?

Legionnaires dz
Pontiac Fever

What media does Legionella grow on?

BCYE

What antimicrobic are natural?

Penicilin G and V

What antimicrobic are penicillinase resistant?

Methicillin
oxacillin
nafeillin

What MO is aminopenicillins effective against?

gram negative bacteria

What are the key aspects of natural antimicrobic?

Inactivated by staphylococcal penicillinases
most staphylococci resistant
Pneumococcal resistance due to altered PBP's

What are the key aspects of penicillinase resistant?

Developed to treat infections caused by penicillinase positive staphylococci
staphylococcal resistance now common due to altered pbp
MRS resistant to all beta lactams

What are the aminopenicllins drugs?

Ampicillin
Amoxicillin

What are the carboxypinicillin drugs?

Carbenicillin
Ticarcillin

What are the Ureidopenicillin drugs?

Azlacillin
Mexlocillin

What are the beta lactam and beta lactamase inhibitor's?

Ampicillin/sulbactam
Ticarcillin/clavulanate

What are the narrow spectrum?

cephalothin
cefazolin

What are the extended spectrum?

Cefamandole
Cefaclor
Cefoxitin

What are the broad spectrum?

Ceftizoxime
Ceftriaxone
Cefoperozone
Cefotaxime
Cefepime
Cefoxitin

What are the carbepenem?

Imipenem

What are the monobactam?

Aztreonam

What are known as first generation?

Cephalothin
Cefazolin

What are known as 2 generation?

Cefamandole,
Cefaclor
Cefoxitin

What are the third generation?

Ceftizoxime
Ceftriaxone
Cefoperozone
Cefotaxime

What are the forth generation?

Cefepime

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