| Term | Definition |
|---|
| formalin, alcohol, or freezing | type of fixative used to prepare tissue for light microscopy |
| osmium tetroxide | type of fixative used to prepare tissue for electron microscopy |
| protein | formalin fixes this type of substance |
| lipid | osmium tetroxide fixes this type of substance |
| perfusion and immersion | the two ways tissue can be fixation |
| perfusion | the fixative is injected into the blood vascular system |
| immersion | the tissue is immersed in the fixative |
| fixation, dehydration, embedding, sectioning, and staining | the first basic steps of tissue preparation |
| Hematoxylin and Eosin (H&E) | common stain for brightfield microscopy |
| hematoxylin | poisitively charged stain that stain |
| eosin | negative charged stain |
| hematoxylin | an object that appears basophilic is stained by what stain |
| eosin | an object that appears acidophilic or eosinophilic is stained by what stain |
| hematoxylin | the nucleus stains with what stain |
| eosin | the cytoplasm stains with what stain |
| microtome | instrument used for cutting sections of tissue |
| 5-7 micrometers | size in which tissue sections are cut for light microscopy |
| 20-80 nanometers | size in which tissue sections are cut for electron microscopy |
| heavy metal salts such as lead citrate and uranyl acetate | type of substance used to stain tissue for electron microscopy |
| periodic acid schiff (PAS) | stain for carbohydrates |
| best carmine | stain for glycogen |
| acid phosphatase | stain for lysosomes |
| oil red or sudan black | stain for lipids |
| janus green | stain for mitochondria |
| silver nitrate | stain for golgi body |
| immunocytochemistry | based on antigen/antibody reaction |
| immunofluorescence | technique used to test antibody reactions |
Combine with other sets
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