microbiology test 4

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daniellesox  on April 15, 2012

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microbiology test 4

radiation preservation
uses ionizing radiation; may affect taste
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radiation preservation uses ionizing radiation; may affect taste
aseptic packaging sterile food packaging
thermophilic anaerobic spoilage canned foods incubated at a high temperature. can develop spoilage from organisms surviving
commercial sterilization similar to autoclave. designed to destroy spores of botulinim
soy sauce soybeans incubated w/ aspergillis to produce sugars
bread s. cerevisiae.
CO2 produced during fermentation causes bread to rise
vinegar oxidation of ethanol by acetic acid bacteria
production of alcoholic beverages wine: fermentation of naturally occuring sugars
beer: starches in grain broken to simple sugars & fermentated by yeast
fermented meats 1) starter cultures of lactobacillus
2) ground meat mixed w/ sugar & salt
pickled vegetables fermentation. employs lactic acid from vegetables. lowers pH & discourages growth
yogurt production 1) pasteurized milk incubated
2) products lactic acid
cheese production 1) milk incubated w/ lactobacillus
2) whey is removed
3) curds are salted & processed
4) cheese is ripened
extrinsic factors affecting microbial growth 1) storage temperature
2) atmosphere
intrinsic factors affected microbial growth 1) water
2) pH
3) nutrient content
4) biological barriers
5) antimicrobial chemicals
xenobiotics synthetic compounds that cannot be broken
bioremediation use of microbacteria to get rid of pollutants
composting natural decomposition of organic solid material
sanitary landfills used for disposal of non-hazardous solid waste
municipal sewage treatment methods 1) activated sludge
2) lagoon
3) trickling filter
coliforms indicator of fecal contamination in water
biological oxygen demand (BOD) measure of biologically degradeable organic matter in water
sewage all household water from washing/toilet waste
carbon cycle 1) carbon fixation
2) respiration/fermentation
carbon fixation CO2 converted into organic form
respiration/fermentation breakdown of organic material
nitrification oxidation of ammonium to nitrate
denitrification reduction of nitrate to nitrogen
nitrogen fixation nitrogen reduced to form ammonia
ammonification decomposition of organic nitrogen into ammonia
nitrogen cycle 1) nitrogen fixation
2) ammonification
3) nitrification
4) denitrification
hydrothermal vents water seeps into ocean floor is heated & released again full of nutrient to serve as food source for organisms
mycorrhizea fungi growing w/ plant roots, increase surface of the plant
C horizon contain lumps of rock
R horizon bedrock
A horizon topsoil. decomposed, usually dark brown
B horizon mineral, subsoil, contains clay
bloom rapid growth of phytoplankton due to change in water
zooplankton microscopic heterotrophs which feed on phytoplankton
phytoplankton free-floating, photosynthetic organisms. largely composed of algea.
thermocline layer seperating epilimmon & hypolimnion
hypolimnion oxygen poor; lower layer due to growth of heterotrophs
epolimnion oxygen rich, upper layer. photoautotrophs
eutrophic waters nutrient rich, autotrophs flourish
oligotrophic waters nutrient poor, little autotrophic growth
detritus partially decayed organic material
decomposers digest producers & consumers, uses detritus as food source
consumers heterotrophs, consume primary producers
primary producers convert CO2 into organic material. serves as food source
niche specific role of an organism within an ecosystem
ecosystem ecological system with population of organism
ecology study of relationship between organisms and the environment
mycobacterium m.avium/ m.intracellulare.
bacteria is phagocytized but not destroyed. characterized by fever & weight loss
pneumocystis pneumonia p.carinii/jiroveci.
fungal, spore inhalation
kaposis sarcoma herpes virus 8. asymptomatic, 50% of HIV patient will develop this
AIDS treatment & prevention inhibitors of reverse transcriptase, protease, integrase, fusion, entry, or a combination of all
epidemiology of AIDS 1) sexual contact
2) blood/blood products
3) vertical transmission
pathogenesis of HIV gp120 & gp41
gp120 mediates viral binding to CD4
gp41 mediates fusion of viral envelope, allowing entry
symptoms of HIV early symptoms resemble flu-like, latent.
latency ends & immune system is weakened & AIDS develop
HIV retrovirus. enveloped, contains reverse transcriptase, ssRNA, spikes
schistosomiasis schistoma. spread in human feces enter water supplies. requires a snail intermediate host
malaria 1) P. vivax
2) P. falicparum
3) P. malariae
4) P. ovale.
characterized by paroxysms
chagas american trypanosomiasis. protozoa. animal reservoir & spread to human by feces
toxoplasmosis toxoplasma gondii. cat is host. cysts are ingested by animals, then humans. causes stillbirth
yellow fever viral. spread by mosquito. bite goes into blood stream and then to liver
mononucleosis epstein-barr virus. fever, sore throat. infects B cells
3 types of typhus 1) epidemic typhus
2) endemic typhus
3) tickborne typhus
Epidemic typhus rickettsia prowazekii. spread by lice
endemic typhus rickettsia typhi. spread by rat flea
tickborne typhus rocky mountain spotted fever
3 clinical manifestations of plague bitten: enlarged lymph nodes, die w/i 1 week
inhaled droplets: cough, bloody sputum, die 1-3 days
bloodstream: release of endotoxin, hemorrhage into skin
plague yersinia pestis. "black plague" fleas are vector to humans
undulant fever "brucellosis" brucella. recurring fever, associated with animals
anthrax bacillus anthracis. produce spores, at risk when handling hides. can be localized or pulmonary.
tularemia francisella tularensis. "rabbit fever" animal bite/scratch causes ulcer
rheumatic fever s. pyogenes. follows strep throat, joint pain/rash, may lead to carditis
puerperal sepsis s.pyogenes. childbirth fever, nosocomial infection of uterus
necrosis death of tissue from ischemia
ischemia interruption of blood flow resulting from a wound
venules return blood to vein
capillaries small vessels, gas exchange
arterioles deliver blood to capillaries
gonorrhea neisseria gonorrhea. discharge in females & males. can lead to ectopic pregnancy & PID
NGU (nongococcal urethritis) discharge & dysuria. infectious form becomes non-infectious form, divides and causes reaction
syphilis treponema pallidum. 3 clinical stages
trichinosis 1) pig ingests trichinella
2) humans ingest undercooked pork
3) cysts develop & produce larvae
4) larvae forms cysts in tissue
hookworms necator americanis. feeds on blood/tissue. may cause pica
pinworms enterobius vermicularis.
1) adult migrates out & causes itching
2) contaminate fingernails
3) eggs ingested & mature
hydatid disease echinicoccus granulosus.
1) dog ingests meat
2) cysts develop
3) humans infected by dog feces
4) eggs migrate in body and develop
tapeworms t. saginata, t. solium
1) eggs excreted
2) eggs ingested by cattle and hatches
3) consumption of undercooked meat
4) larvae develop in adults
amoebic dysentery entamoeba histolytica. bloody feces
giardiasis g. lamblia (protozoa). ingestion of cysts leads to infection. lakes and ponds
hepatitis C mild symptoms. transmitted by exposure to blood. no vaccine
hepatitis B transmitted by contact w/ body fluids. leads to cirrhosis or liver failure
hepatitis A fecal-oral route. jaundice and clay-colored feces. vaccination
Cytomegalovirus inclusion disease (CMV) human herpes virus 5. nuclear swelling, infected for life. sexually, transfused blood
mumps infection of parotid glands via droplet transmission
yersinia gastroenteritis y. entercolitica, y. pseudotuberculosis. transmitted in meat/milk. severe abdominal pain associated with appendicitis
campylobacteriosis e. jejuni. leading cause of bacterial diarrhea in US. uncooked poultry
travelers diarrhea e. coli gastritis. shiga-toxin producing results from lysogeny & cause a/e lesions
cholera vibrio cholera. large infectious dose. cholera toxin causes cell to secrete electrolytes. characterized by rice stools
shigellosis bacillary dysentery, s. dysenteriae. small infectious dose. bacteria replicate and destroy mucosa
typhoid fever salmonella typhi. spread in human feces. bacteria multiply & spread to blood.
salmonellosis s. enterica. food products contaminated with animal feces. weak ID50. killed by stomach acid
staph food poisoning intoxication. super antigen triggers vomitting reflex
virulence factors for helicobacter 1) urease
2) motility
3) CaGa
4) VacA
helicobacter pylori gastritis inflammation of stomach. leads to ulcers & cancer
intoxication ingestion of microbial toxin
infection pathogen enters GI tract and multiplies
gingivitis infection of gums
periodontal disease inflammation & degeneration of structures supporting teeth
dental caries tooth decay, plaque leading to deterioration of enamel
dental plaque biofilm formation containing various speices of bacteria
2 phases of leptospirosis first phase: 1) flu like symptoms
2) red eyes
second phase: 1) recurrence of symptoms
2) vomitting and rash
leptospirosis transmitted through mucous membranes by animal urine
pyelonephritis inflammation of kidneys
pyuria accumulation of leukocytes in the urine
dysuria pain during urination
cystitis inflammation of urinary bladder, mostly cause by e.coli. includes dysuria & pyuria
kuru smearing of brain tissue on body
sheep scrapie animal scrapes against fence until skin is raw. loss of motor control then death
african sleeping sickness t. bruccei rhodesiense (acute)
t. bruccie gambiense (chronic)
transmitted by tsetse flies
cryptococcus c. neoformans (unhealthy people)
c. gattii (healthy people)
transmitted through inhalation. very thick capsule
rabies zoonotic illness. fever, nausea, tingling sensation
poliomyelitis poliovirus. 3 t ypes. transmitted orally. muscle spasms and weaken bone. vaccine
leprosy mycobacterium leprae. tuberculoid & lepromatous. transmitted via direct contact
botulism intoxication of a-b toxin. clostridium botulinum. dizziness and blurred vision & paralysis of voluntary muscles
tetanus clostridium tetani. potent neurotoxin. muscle spasms
listeria l. monocytogenes. associated w/ lunchmeats, outcome is meningitis
pneumococcal meningitis streptococus pneumoniae. damage leads to inflammation. mortality and brain damage
hemophilus meningitis HIB vaccine reduced rate in children. encapsulated strains cause it
meningococcal meningitis neisseria meningitis, airborne transmission. symptoms include petechia.
common symptoms of meningitis fever, stiff neck, nausea/vomitting, convulsions & coma
encephalitis if brain is infected by meningitis
blood brain barrier tightly packed cells, impermeable
meninges 3 layer covering of brain & spinal cord
neurons individual nerve cell
trichomoniasis t. vaginalis. protozoan. itching & smelly discharge
genital warts human papilloma virus, growth on genitalia. can cause cervical virus
herpes 2 burning/itching, blisters. infectious for life.
chancroid haemophilus ducreyi. painful genital sores
neurosyphilis syphilus various mental & psychological disorders
cardiovascular syphilus aneurisms resulting from chronic inflammation
gummatous syphillis development of lesions called gumma
urease converts urea to ammonia
CagA may be related to cancer development
VacA increase urea production by muscosal cells
lipooligosacharide activates complement leading to inflammation that causes symptoms of gonorrhea
primary stage of syphilis cancer at site of infection on genitalia, painless
secondary stage of syphilis rash on palms and soles of feet several weeks after primary stage
tertiary stage of syphilis gummatous, cardiovascular, neurosyphilis
tuberculoid leprosy cell mediated immunity develops & macrophages limit spread
lepromatous leprosy disseminated infection, cooler parts of body affected first
sabin vaccine attenuated vaccine
salk vaccine inactivated
pathogenesis of rabies virus enters sensory neuron to travel to spinal cord and brain
Creutzfeldt-Jakob disease fatal within 1 year. presents with behavorial/personality changes, lack of muscle control, dementia
lymph "recycled plasma"
virulence factors of the plague evasion of phagocytosis & impairing complement
lifecycle of malaria sporozoites injected into blood stream, infect liver and enter rbc, produce merozoites, rbc ruptures and cycle repeats

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