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black

starch & iodine

red

phenol red in neutral

red

phenol red in 7+

yellow

phenol red in acid

yellow

phenol red in -7

MR

test for acid

yellow

methyl red in neutral

yellow

methyl red negative result

red

methyl red in acid

red

methyl red positive result

VP

tests for acetoin

red

VP test positive

negative

VP test with no color change

OF medium

HIGH concentration of carbohydrate and LOW concentration of peptone

bromothymol blue

indicator in OF medium

yellow

bromothymol blue in the presence of acid

yellow

bromothymol blue, carbohydrate has been catabolized

blue

bromothymol blue, ammonia production

50

nutrient gelatin dissolves at

20

nutrient gelatin solidifies at

gelatinase

nutrient gelatin does not solidify even when cooled, after being hydrolized with this

urease

liberates ammonia from urea

phenol red

used to test for presence of urease

MRVP

used to distinguish between organisms that produce large amounts of acid and those who produce neutral product acetoin

urea

waste product of protein digestion

urea agar

contains peptone, glucose, urea, phenol red

urea agar

the ph is 6.8

pink

urea agar turns this color during ammonia production

ammonia

the ph is 8.4 when this is present

ammonia

when amino acid gets deaminated, you get

green

indicates ferric ion

green

positive for phenylamine deamination

nessler

reagent, indicating ammonia

yellow

in nesslers reagent, indicates ammonia

MIO

motility indole ornithine media

ornithine

tests for carboxylation

purple

positive ornithine test

yellow

negative ornithine test

carboxylation

removal of co2 from amino

positive

ornithine test results if the ph has shifted to basic

indole

production of this indicates a breakdown of tryptophan

kovac's

reagent used in indole test

pink ring

indicator of indole production

motility

large amount of spread away from the stab indicates

black

precipitate is formed when h2s is produced

peptone iron agar deep

medium in sulfur reduction

sulfhydrase

break down of sulfur

halo

if the bacteria uses starch, this is the indicator

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