Microbiology Test Tres

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Microbiology McVay Auburn Final

The terminal electron acceptor in forms of bacterial respiration may be:
a. Oxygen
b. NAD
c. an Organic molecule
d. an inorganic molecule

The terminal electron acceptor in forms of bacterial respiration may be oxygen or inorganic molecules.

The name given to the reaction involving addition of electrons or hydrogen atoms to a compound is termed:
a. Oxidation
b. Reduction
c. Respiration
d.Fermentation
e. Glycolysis

The name given to the reaction involving addition or electrons or hydrogen atoms to a compound is termed: reduction.

The most common starting pathway for the breakdown of sugars is
a. Kreb's cycle
b. Fermentation
c. Glycolysis
d. Oxidation
e. All above

Glycolysis is the most common starting pathway for the breakdown of sugars.

Which of the following processes generates the greatest amount of energy?
a.Glycolysis
b. Fermentation
c. Aerobic Respiration
d. Phosphate Pentose Pathway

Aerobic respiration generates the greatest amount of energy.

Fermentation:
a. uses organic molecules as electron acceptors
b. uses inorganic molecules as electron acceptors
c. is necessary to generate oxidized forms of electron carriers
d. Is necessary to produce NADPH
e. Both a and C

Fermentation uses organic molecules as electron acceptors and is necessary to generate oxidized form of electron carriers.

Pyruvate can be metabolized along two major routes.They are:
a. fermentation and respiration
b. reduction and oxidation
c. metabolism and anabolism
d. none
e. all

Pyruvate can be metabolized by fermentation and respiration

The use of the suffix "ase" on a word denotes an:
a. substrate
b. product
c. enzyme
d. electron acceptor
e. electron donor

ase= enzyme

Glycolysis
a. requires oxygen
b. produces only 3 molecules of ATP
c. Produces 4 molecules of NAD
d. May occur under aerobic or anaerobic conditions
e. Both b and c are correct

Glycolysis may occur under aerobic or anaerobic conditions

The transition step
a. links glycolysis to the pentose phosphate pathway.
b. links the TCA cycle to the electron transport system
c. links glycolysis to the TCA cycle
d. Produces a molecule of inorganic carbon
e. both C and D are correct

The transition step links glycolysis to the TCA cycle and produces a molecule of inorganic carbon

The TCA cycle produces
a. ATP through substrate level phosphorylation
b. ATP through oxidative phosphorylation
c. Reducing power
d. All above
e. only a and c are correct

The TCA cycle produces ATP through substrate level phosphorylation and it produces reducing power.

In the mitochondrial electron transport system:
a. NADH and FADH2 donate electrons at the same place
b. NADH donates electrons in complex I
c. FADH2 donates electrons in complex III
d. None correct
e. only b and c above are correct

In mitochondrial electron transport system, NADH donates electrons in complex I

The production or ATP via the Electron transport system, in either eukaryotes or prokaryotes, requires a membrane. True or False

TRUE TRUE TRUE

The proton motive force
a. Is used to synthesize ATP
b. Is used to drive prokaryotic flagellar roation
c. is used to synthesize NADH
d. all
e. a and b only

Proton motive force is used to synthesize ATP and is used to drive prokaryotic flagellar rotation

Rotating the following answers any way necessary, but no changing their sequence or orientation, which is/are complementary to the sequence 5'AGGCUAAC3'?
a. 5'TCCGATTG 3'
b. 5'CTTAGCCT3'
c. 3'TCCGATTG5'
d. 3'TAAGCTTA5'

c. 3'TCCGATTGS5' is complementary to 5'AGGCUAAC3'

GCCCAAAG is a molecule of
a. DNA
b. RNA
c. Part of a transcript
d. Only a and b
e. all above

all above

Has free 3' OH
a. DNA
b. RNA
c. both

DNA has a free 3' Oh

Has a free 2' OH
a.DNA
b. RNA
c. both

RNA has a free 2' OH

Has a promotor Region
a. RNA
b. DNA
c. both DNA and RNA

c. both

Has a ribosome binding site
a. DNA
b. RNA
c. BOTH

RNA has a ribosome binding site

The 3' end of DNA
a. refers to the OH group on the #3 carbon of deoxyribose
b. Attaches to the 5' phosphate group of the incoming nucleotide
c. Always has a thymine attached to it
d. Always has an adenine
e. only a and b

the 3' end of DNA refers to the OH group on the #3 carbon of deoxyribose and attaches to the 5' phosphate group of the incoming nucleotide

the complementary sequence to TTAC is GTA

yup

Which is true about prokaryotic RNA polymerase
a. It is used during transcription
b. it does not require a primer
c. it is part of a complex including a sigma factor that binds to the promoter region of DNA
d. it reads the template in a 5' to 3' direction
e. answers a-c correct

Prokaryotic RNA polymerase is used during transcription. It doesn't require a primer. IT is part of a complex including a sigma factor that binds to the promoter region of DNA

DNA replication in prokaryotes is usually unidirectional. T or F.

that **** is false

There are ___ codons that code for one of the 20 possible amino acids
a. 60
b.64
c. 20
d. 61
e. 30

d. 61

The ribosomes
a. move along the tRNa in a 3'-5' direction
b. move along the mRNA in a 5'-3' direction
c. move along the DNA in a 5'-3' direction
d. none
e. all

Teh ribosomes move along the mRNa in a 5'-3' direction

In prokaryotes, the first amino acid borne by a tRNA is____and binds to the ____site in the ribosome

N-forml-methionine, P site

Insertional Mutation
a) silent mutation
b) missense mutation
c)nonsense mutation
d) transposon
e)Intercalating agent

Insertional Mutation is a transposon

Addition of nucelotide to DNA strand
a) silent mutation
b) missense mutation
c)nonsense mutation
d) transposon
e)Intercalating agent

Addition of nucleotide to a DNA strand is Intercalating agent

Nucleotide Substitution with amino acid change
a) silent mutation
b) missense mutation
c)nonsense mutation
d) transposon
e)Intercalating agent

Nucelotide substitution with amino acid change is a missense mutation.

Nucleotide substitution with no amino acid change:
a) silent mutation
b) missense mutation
c)nonsense mutation
d) transposon
e)Intercalating agent

Nucleotide substitution with no AA change is a silent mutation

Nucleotide substitution resulting in shortened protein is
a) silent mutation
b) missense mutation
c)nonsense mutation
d) transposon
e)Intercalating agent

Nonsense mutation

Chemical mutagens that mimic the naturally occurring bases are called:
a. alkylating agents
b. Intercalating agents
c. Base Analogs
d. Nitrous oxide

Chemical mutagens that mimic the naturally occurring bases are called:
base analogs

Which of the following DNA strands would UV radiation have the most effect on?
a. AACCGGG
b. TATATACG
c. AUAUCGAU
d. AATTAGTTC
e. both b and d above

AATTAGTTC would be most effected by UV radiation

What is not true about mismatch repair?
a. it utilizes an endonuclease
b. it requires DNa polymerase
c. It requires DNA ligase
d. It replaces both the new and template strand of affected DNA
e. none

replaces both new and template strand of affected DNA

Mutations
a. may occur spontaneously
b. are always harmful
c. can result in resistance to antibiotics
d.all
e. only a and c are correct

Mutations may occur spontaneously and can result in resistance to antibiotics.

Direct selection of mutation involves inoculating cells onto growth media on which
a. the mutant but not the parental cell type will grow.
b. The mutation will be reversed
c. the nutrients needed for mutation to occur are present
d. the mutagen is present

the mutant but not the parental cell type will grow during direct selection of mutation

Indirect selection of a mutant
a. is necessary to isolate auxotrophic mutants
b. requires medium on which the mutant, but not the parental type will grow
c. uses media upon which neither the parental cell type or mutant grows
d. both a and b above are correct
e. both a and c above are correct

Indirect selection of a mutant is necessary to isolate auxotrophic mutants

The ames test identifies potential carcinogens by measuring the rate of generation of auxotrophic mutants. TRUE OR FALSE

False

In performing the Ames test using His- auxotrophs, if a chemical is a mutagen you would expect
a) an increase in auxotrophs
b) an increase of His+ cells
c) an increase in revertants
d) both a and b
e) both b and c

You would expect both an increase of His+ cells and an increase in revertants.

What part of the attached bacteriophage enters through the host cell envelope
a)the capsid and nucleic acid
b) The capsid only
c) the nucleic acid
d) the enzymes necessary for replication of the virus

The nucleic acid only

An infection resulting when a phage replicates inside the host cell and then lyses its host during its release
a) is a productive infection
b) is a lysogenic infection
c) is a lytic infection
d) a and b
e) a and c

It is a productive infection and a lytic infection

What part of E. Coli T4 phage attaches to the host cell receptors?
a) spike proteins of the envelope
b) fiber proteins of the tail portion
c) spike proteins of the caspid
d) genomic DNA

Fiber proteins of the tail portion attach to the host cell receptors

Filamentous phage
a) cause a productive infection
b) infect only F- cells
c) infect only F+ cells
d) a and b
e) a and c

Filamentous phage cause a productive infection and infect only F+ cells

The bacterial viruses that are extruded (leaked) from a bacterial cell, but do not kill the cell
a) are filamentous
b) are M13 phage
c) are temperate
d) are called lambda phage
e) both a and b above are correct

are filamentous and M13 phage

Bacterial DNa is protected from restriction enzymes that the bacterium produces by being
a) coated with a membrane
b) made into double-stranded RNA
c) Methylated
d) Acetylated
e) none above

bacterial DNA protected by being methylated

Once inside the host cell, lytic phage DNA
a) is replicated
b) can be digested by restriction endonucleases
c) is transcribed
d) all above

lytic phage DNA (once inside the host cell) is replicated, can be digested by restriction endonucleases and is transcribed

The host range of a bacteriophage depends on the presence of host receptor molecules. True or False

TRUE TRUE

An antibiotic is added to a culture of E. Coli, resulting in death of the cells. Bacteriophage are then added. What could then occur?
a) Attachment
b) Penetration
c) Replication, assembly, and release
d)all
e) a and b

Attachment, Penetration, "Replication, assembly, and release" could all occur

Which of the following means of DNa repair mechanisms does not require replacement of the damaged DNA with newly synthesized DNA?
a) Dark repair of thymine dimers
b) mismatch repair
c) Light repair of thymine dimers
d)all
e) none

Light repair of DNA does not require replacement of the damaged DNA with newly synthesized DNA.

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