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5 Written Questions

5 Matching Questions

  1. dense fibrillar component
  2. replicons
  3. RNA primase
  4. activating amino acid as amion-acyl group
  5. polyribosomes/polysomes
  1. a initiates DNA replication on both strands by adding RNA primers
    -1 for the leading strand, 1 for each Okazaki fragment
  2. b uses energy from hydrolysis of ATP (ATP ->ADP+Pi)
  3. c rRNA transcribed at border of fc and here
    -ribosomal proteins associate here to form pre-rRNP particles
  4. d -several attached ribosomes associated to one mRNA
    -contain functional components of mRNA, tRNA and ribosomes
  5. e Individual units of replication
    -avg size of 150 kbp

5 Multiple Choice Questions

  1. ribosomes transcribing same piece of mRNA creating many copies of same protein
    -5' end codes for N-terminal amino acid
    -3' end codes for C-terminal amino acid
  2. TFIIA and TFIIB which lead to recruitment of TFIIF/pol II and other TFs
  3. region of chromosomes where rRNA genes are amplified (>100 copies)
  4. -add poly(A) tail (RNA with only adenine stretch) to RNA
    -immediately after termination
  5. 1 DNA labeled with high-conc. radioactive thymidine followed by lower concentration
    2 cells are lysed and DNA spread out as fivers on glass slide and exposed for autoradiography
    -enables ID of DNA replication sites and direction
    -proves bidirectional replication

5 True/False Questions

  1. DNA helicaseseals okazaki fragments on lagging strand.

          

  2. major genomic functions of eukaryotic cells1)RNA primer synthesis by RNA primase
    2)elongation by DNA polymerase III
    3)primer removal and gap filling by DNA polymerase I

          

  3. mitotic chromosomesribosomes transcribing same piece of mRNA creating many copies of same protein
    -5' end codes for N-terminal amino acid
    -3' end codes for C-terminal amino acid

          

  4. taylor experiment-proved semiconservative DNA replication in eukaryotic cells
    -labeled metaphase chromosomes had only 1 sister chromatid labelled

          

  5. "linker DNA"core particles connected by this
    DNA wrapped +linker=200 bp

          

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