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5 Written Questions

5 Matching Questions

  1. DNA replication in prokaryotes
  2. key components of pre-initiation complex (2)
  3. nucleolar organizer regions (NOR)
  4. pre-Initiation of Protein Syntheisis
  5. DNA polymerases
  1. a TFIIA and TFIIB which lead to recruitment of TFIIF/pol II and other TFs
  2. b - catayst of DNA replication
    -can only add nucleotides to pre-existing "primer" (this is where RNA primer comes in)
  3. c 1-codon AUG near 5' end of mRNA
    2-aminoacyl-tRNA binds to P site with aa methionine
  4. d region of chromosomes where rRNA genes are amplified (>100 copies)
  5. e -all DNA in single circular chromosome
    -proceeds in both directions from ori (origin sequence) to T (termination site)

5 Multiple Choice Questions

  1. rRNA transcribed at border of fc and here
    -ribosomal proteins associate here to form pre-rRNP particles
  2. -rDNA genes concentrated here
    -numerous of these compose the nucleolus
  3. initiation factors
    -TFIID [TATA BP & TAFs (transcription associating factors)],
    -RNA polymerase II (pol II) and
    -other TFs
  4. 1: bring ribosomal subunit to initiation codon(codon AUG near 5' end of mRNA)
    2: bring 1st aa tRNA into ribosome (methionine-tRNA enters P site ;binds to AUG codon and IF2 )
    -IF3 and IF1 released
    3: assemble complete initiation complex(large subunit joins complex; IF2 hydrolyzed through GTP hydrolysis, releasing IF2-GDP)
  5. IF1-promotes attachment of subunit to mRNA
    IF2 (a GTP binding protein)- required for attachment of 1st aa-tRNA
    IF3-prevent large subunit from joining prematurely to small subunit

5 True/False Questions

  1. final mRNA structure (4)-2 copies each of core histones H2A, H2B, H3, H4
    "the core particle" (doesn't include H1)

          

  2. termination of protein synthesis1-codon AUG near 5' end of mRNA
    2-aminoacyl-tRNA binds to P site with aa methionine

          

  3. aminoacyl-tRNA synthetase enzyme (aaRS)section of mRNA before the start codon and after stop codon (not translated)
    -mRNA stability, localization and translation

          

  4. DNA replication steps-semi conservative(parent chain separate and daughter chain base pair with parent chains forming 2 new double helices)
    -antiparallel(5'->3' on both but in opposite directions to each other)

          

  5. snRNP and splicesome complexescomposed of many splicing factors
    -mediate splicing process

          

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