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5 Written questions

5 Matching questions

  1. chromatin fiber
  2. breaking down chromatin into DNA and histones
  3. pre-Initiation of Protein Syntheisis
  4. properties specific to eukaryotic DNA replication(4)
  5. IF (initiation factors) involved in prot. synthesis
  1. a 30 nm fiber after 10 nm nucleosome filament coils; helical structure
  2. b IF1-promotes attachment of subunit to mRNA
    IF2 (a GTP binding protein)- required for attachment of 1st aa-tRNA
    IF3-prevent large subunit from joining prematurely to small subunit
  3. c 1-codon AUG near 5' end of mRNA
    2-aminoacyl-tRNA binds to P site with aa methionine
  4. d 1-nuclease digests linker DNA
    2-dissociation with high salt concentration
  5. e -replicon organization along chromosomes
    -replication timing during S-phase
    -initiation of DNA replication during S-phase
    -organization of replication sites in cell nucleus

5 Multiple choice questions

  1. -initiates at original sequences and proceeds in both directions along DNA
    -leading goes bonds toward parent DNA, lagging bonds in opposite direction
  2. Individual units of replication
    -avg size of 150 kbp
  3. composed of many splicing factors
    -mediate splicing process
  4. -lowest level of chromosome organization
    -form fundamental repeating units of eukaryotic chromatin
    -146bp of DNA wrapped almost twice around histone octomer
    -10 nm fibers
  5. keeps DNA single stranded

5 True/False questions

  1. activating amino acid as amion-acyl group-3 in large (60S)subunit(28S, 5.8S, 5S);
    -1 in small (40S)subunit(18S)
    -40S+60S make 80S ribosome (there is no typo lol)


  2. Post-transcriptional modifications (3)-process for converting pre-RNA to final RNA
    1: 5' capping (methylated guanine)
    2: 3' polyadenylation
    3: RNA splicing


  3. aminoacyl-tRNA synthetase enzyme (aaRS)5' cap, coding regions (CDS), untranslated regions (UTRs), 3' poly (A) tail


  4. major genomic functions of eukaryotic cells-all DNA in single circular chromosome
    -proceeds in both directions from ori (origin sequence) to T (termination site)


  5. fibrillar centers (fc)-processing of pre-rRNP particles occurs here
    -mature ribosomal subunits released for transport to cytoplasm


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