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5 Written questions

5 Matching questions

  1. nucleolar organizer regions (NOR)
  2. elongation is energy dependent
  3. DNA Ligases
  4. ribosomal binding sites
  5. replication factories
  1. a DNA replication sites anchored to nuclear matrix
    -contains cluster of DNA replication loops
  2. b region of chromosomes where rRNA genes are amplified (>100 copies)
  3. c for each elongation cycle, 2 GTP hydrolyzed
    -20 sec to synthesize 240 aa
    -3ATP for each aa
  4. d A (binds aminoacyl tRNA ), P (binds peptidyl-tRNA), E (binds free tRNA before it exits ribosome)
  5. e seals okazaki fragments on lagging strand.

5 Multiple choice questions

  1. -proved semiconservative DNA replication in eukaryotic cells
    -labeled metaphase chromosomes had only 1 sister chromatid labelled
  2. - catayst of DNA replication
    -can only add nucleotides to pre-existing "primer" (this is where RNA primer comes in)
  3. -rDNA genes concentrated here
    -numerous of these compose the nucleolus
  4. -linker histone; seals off nucleosome at location at which linker DNA enters/leaves
    -involved in packing "beads on a string"
  5. uses energy from hydrolysis of ATP (ATP ->ADP+Pi)

5 True/False questions

  1. untranslated regions (UTRs)section of mRNA before the start codon and after stop codon (not translated)
    -mRNA stability, localization and translation


  2. dense fibrillar component-rDNA genes concentrated here
    -numerous of these compose the nucleolus


  3. alternative splicingIndividual units of replication
    -avg size of 150 kbp


  4. properties specific to eukaryotic DNA replication(4)-replicon organization along chromosomes
    -replication timing during S-phase
    -initiation of DNA replication during S-phase
    -organization of replication sites in cell nucleus


  5. termination of protein synthesis1: bring ribosomal subunit to initiation codon(codon AUG near 5' end of mRNA)
    2: bring 1st aa tRNA into ribosome (methionine-tRNA enters P site ;binds to AUG codon and IF2 )
    -IF3 and IF1 released
    3: assemble complete initiation complex(large subunit joins complex; IF2 hydrolyzed through GTP hydrolysis, releasing IF2-GDP)


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