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5 Written questions

5 Matching questions

  1. taylor experiment
  2. rRNA
  3. "linker DNA"
  4. RNase H
  5. chromatin organization on nuclear matrix
  1. a core particles connected by this
    DNA wrapped +linker=200 bp
  2. b central component of ribosome's protein manufacturing machinery
  3. c chromatin loops are attached to nuclear matrix
  4. d removes RNA which is then filled in with DNA nucleotides
  5. e -proved semiconservative DNA replication in eukaryotic cells
    -labeled metaphase chromosomes had only 1 sister chromatid labelled

5 Multiple choice questions

  1. many nucleosomes on DNA strand
  2. 1: bring ribosomal subunit to initiation codon(codon AUG near 5' end of mRNA)
    2: bring 1st aa tRNA into ribosome (methionine-tRNA enters P site ;binds to AUG codon and IF2 )
    -IF3 and IF1 released
    3: assemble complete initiation complex(large subunit joins complex; IF2 hydrolyzed through GTP hydrolysis, releasing IF2-GDP)
  3. most actively transcribed RNA in cell
    -composes <80% total RNA content
    -highly amplified on 5 human chromosomes containing 100s of genes
  4. -3 in large (60S)subunit(28S, 5.8S, 5S);
    -1 in small (40S)subunit(18S)
    -40S+60S make 80S ribosome (there is no typo lol)
  5. -protects 5' end of primary RNA transcript from attach by exonucleases
    -regulates nuclear export
    -promotes translation

5 True/False questions

  1. granular component (gc)3 nucleotides complementary to 3 nucleotides of on mRNA


  2. aminoacyl-tRNA synthetase enzyme (aaRS)attach amino acids to tRNAs by high energy amino acyl bonds


  3. assembling complex includes (3) :-codons decoded and translated to protein
    -begin with start codon, end at stop codon


  4. ribosomal binding sitesA (binds aminoacyl tRNA ), P (binds peptidyl-tRNA), E (binds free tRNA before it exits ribosome)


  5. Kozak boxribosome identifies start codon using this in eukaryotes


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