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5 Written questions

5 Matching questions

  1. histone octamer (4)
  2. ribosomal binding sites
  3. breaking down chromatin into DNA and histones
  4. initiation of protein synthesis
  5. replicons
  1. a A (binds aminoacyl tRNA ), P (binds peptidyl-tRNA), E (binds free tRNA before it exits ribosome)
  2. b 1-nuclease digests linker DNA
    2-dissociation with high salt concentration
  3. c 1: bring ribosomal subunit to initiation codon(codon AUG near 5' end of mRNA)
    2: bring 1st aa tRNA into ribosome (methionine-tRNA enters P site ;binds to AUG codon and IF2 )
    -IF3 and IF1 released
    3: assemble complete initiation complex(large subunit joins complex; IF2 hydrolyzed through GTP hydrolysis, releasing IF2-GDP)
  4. d Individual units of replication
    -avg size of 150 kbp
  5. e -2 copies each of core histones H2A, H2B, H3, H4
    "the core particle" (doesn't include H1)

5 Multiple choice questions

  1. region of chromosomes where rRNA genes are amplified (>100 copies)
  2. initiation factors
    -TFIID [TATA BP & TAFs (transcription associating factors)],
    -RNA polymerase II (pol II) and
    -other TFs
  3. chromatin loops are attached to nuclear matrix
  4. ribosome identifies start codon using this in prokaryotes
  5. 30 nm fiber after 10 nm nucleosome filament coils; helical structure

5 True/False questions

  1. pre-Initiation of Protein Syntheisis1: bring ribosomal subunit to initiation codon(codon AUG near 5' end of mRNA)
    2: bring 1st aa tRNA into ribosome (methionine-tRNA enters P site ;binds to AUG codon and IF2 )
    -IF3 and IF1 released
    3: assemble complete initiation complex(large subunit joins complex; IF2 hydrolyzed through GTP hydrolysis, releasing IF2-GDP)

          

  2. rRNAremoves RNA which is then filled in with DNA nucleotides

          

  3. Meselson & Stahl Experiment (1959)-processing of pre-rRNP particles occurs here
    -mature ribosomal subunits released for transport to cytoplasm

          

  4. steps to eukaryotic transcription of pre-mRNA(4)-process for converting pre-RNA to final RNA
    1: 5' capping (methylated guanine)
    2: 3' polyadenylation
    3: RNA splicing

          

  5. histone H1-linker histone; seals off nucleosome at location at which linker DNA enters/leaves
    -involved in packing "beads on a string"

          

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