Microbiology Ch. 3

About this set

Created by:

Ngann1  on June 1, 2012

Log in to favorite or report as inappropriate.
Pop out
No Messages

You must log in to discuss this set.

Microbiology Ch. 3

Living Organisms
1. growth
2. reproduction
3. responsiveness
4. metabolism.
1/60
Preview our new flashcards mode!

Study:

Cards

Speller

Learn

Test

Scatter

Games:

Scatter

Space Race

Tools:

Export

Copy

Combine

Embed

Order by

Terms

Definitions

Living Organisms 1. growth
2. reproduction
3. responsiveness
4. metabolism.
Prokaryotic cells Have 1. Ribosome 4.Inclusions
2. Cytoplasm 5.Cell wall
3. Flagellum 6. Nucleoid
Cell wall Prevents osmotic lysis and gives shape
Made of peptidoglycan (only in bacteria)
All cells have 1. Ribosomes 4.DNA=genetic material
2. Cell membrane
3. Cytoplasm
Prokaryotic cell structure a) Bacteria and archea
i) lack nuclei (membrane bound organelles)
ii) binary fission
Eukaryotic cell structure i) lack cell wall
ii) Cytoskeleton
iii) mitosis
Glycocalyx -composed of polysacharides, polypeptides or both
-survive and cause disease
-sticky allow bacteria to adhere to other cells
-has moisture to prevent cell from drying out
-May hide a cell - unrecognized by the immune system
Slime layer loose, water soluble glycocalyx
Capsule A glycocalyx when repeating units of organic chemicals attached to the cell surface
Flagella A cell's motility, long structures that extend beyond the
surface of a cell and its glycocalyx and propel the cell through
its environment
Flagella Composed of 1. a long, thin filament
2. a hook
3. a basal body
Flagella Motility/Functions Chemotaxis- Motile through sensing chemicals
If chemical compound is nutrient, Acts as attractant
If compound is toxic, Acts as repellent
Flagella rotation responsible for run and tumble movement of bacteria
Gram + Flagella Peptidoglycan layer (cell wall)
Rod
Protein Rings
Cytoplasmic membrane
Gram - Flagella Basal Body
Cytoplasmic membrane
Cell wall (outer membrane and Peptidoglycan
layer)
Basal Body Outer protein rings
Inner protein rings
Rod
Integral protein
Fimbriae sticky, bristlelike projections, to adhere to one another and to
substances in the environment. Shorter than flagella
Fimbriae Function used to move across a substrate or toward another bacterium via a process similar to pulling an object with a rope
pilus A special type of fimbria. Also called conjugation pilus. Pili are longer than other fimbriae and usually shorter than flagella.
conjugation pilus mediate the transfer of DNA from one cell to the other
via a process termed conjugation
Bacterial Cell Wall Gram - and Gram +
Alternating series of NAG and NAM which is the backbone of the peptidoglycan (found only in bacteria)
Chains are attached by cross-bridges of 4 amino acid
Gram + cell wall thick layer
Teichoic Acid: covalently linked to lipids forming lipoteichoic acid (anchors the peptidoglycan to the cytoplasmic membrane)
Gram - cell wall Thin layer of peptidoglycan
-Periplasmic space (chemical reactions occur)
-Porin (integral proteins that regulates what comes in/out of the periplasmic space)
-Inner leaflet-phospholipids + proteins
-Outer-lipopolysaccharide (LPS)
Transport Across Plasma membrane 1. Simple diffusion
2. Facilitated Diffusion
3. Osmosis
4. Active Transport
5. Group Trans-location
Passive Transport diffusion, facilitated diffusion, and osmosis
Simple Diffusion High to low concentration
Facilitated Diffusion proteins act as channels or carriers to allow certain molecules to diffuse down their concentration gradients into or out of the cell. High to low
Osmosis diffusion of water across a selectively permeable membrane, across a membrane that is permeable to water molecules, but not to solutes that are present, such as proteins, amino acids, salts, or glucose. Continues until equilibrium is reached, water goes into cell and cell wall keeps it from bursting
Hypertonic solution has a lower concentration of water; a higher concentration of solutes . Water will move out, a cell will lose water and shrivel
Hypotonic As water moves into the cell, water pressure
against its cytoplasmic membrane increases, and the cell
expands.
Active transport Molecules more against the concentration gradient. Low to high. Energy required (ATP or proton motive force)
Group Translocation Active process, occurs in some bact. Substance being transported is chemically changed.
cytoplasm of Bacteria cytosol, inclusions, ribosomes, and in many cells, a cytoskeleton
Cytoplasmic Membrane Structure is a lipid bilayer with embedded proteins
Integral and peripheral proteins
Bilayer consists of two opposing leaflets
Leaflets composed of phospholipids
Cytoplasmic membrane functions Selective permeability
Maintain concentration gradients
Energy transformations
Cytoplasm Cytoplasm is the gel-like substance inside the plasma membrane.
Cytoplasm Internal Structures 1. Chromosome: Resides in cytoplasm in nucleoid space
2. Plasmid
3. Ribosome- Involved in protein synthesis, composed of large and small subunits
prokaryotic ribosomal subunits Large = 30S Small = 50S Total = 70S
Larger than eukaryotic ribosomes
40S, 60S, 80S
Difference often used as target for antimicrobials
Plasmid Extrachromosoma, Independently replicating
Encode characteristic, Potentially enhances survival
Antimicrobial resistance
Cytoplasm contains 1. cytosol
2. ribosomes
3. inclusions
4. cytoskeleton
5. endospores
Cytosol -liquid portion of cytoplasm, mostly water, but contains dissolved and suspended substances (ions, carbohydrates, proteins (mostly enzymes), lipids, and wastes)
-contains the cell's DNA in a region called the nucleoid.
-a distinctive feature of prokaryotes is lack of a phospholipid membrane surrounding this DNA.
inclusions Deposits, are often found within bacterial cytosol.
Rarely, a cell surrounds its inclusions with a polypeptide
membrane. Inclusions may include reserve deposits of lipids,
starch, or compounds containing nitrogen, phosphate, or sulfur.
Endospores durability and potential pathogenicity
constitute a defensive strategy against hostile or unfavorable
conditions.
Endospores used for survival 1. Dormant cell types- remain dormant for 100 years
2. Resistant to damaging conditions- Heat, desiccation, chemicals, UV light
3. Vegetative cell
Vegetative Cell vegetative cell transforms itself into an
endospore only when one or more nutrients (such as carbon or nitrogen) are in limited supply
Vegetative cell produced through germination -Germination occurs after exposure to heat or chemicals
-Germination not a source of reproduction
Endospore structure coat, outer membrane, cortex, germ cell wall, inner membrane, core
Endospore formation Complex, ordered sequence
called sporulation
Sporulation Bacteria sense starvation and begin sporulation
Growth stops, DNA duplicated, Cell splits
Cell splits unevenly, Peptidoglycan between membranes forms core wall and cortex, Mother cell proteins produce spore coat, Mother cell degrades and releases endospore
Spherical Cocci (coccus)
Rod Bacillus
Spiral Spirochaeles
Prefix 1. Diplo - pairs
2. Staphylo - clusters (divides in several planes at random)
3. Strephto - chains (cell divides in one plane)
Packets cell divides in two or more planes, perpendicular to one another
Vibrio Spiral, comma shaped
Spirillum Semi-wavey
Spirochete Really wavey
Other shapes square, star, appendage, filamentous
phototaxis Movement in response to a stimulus is termed taxis. The
stimulus is light
Acid fast bacteria Gram-positive cells that contain large amounts of waxy lipids.
mycoplasma lack cell walls entirely. In the past, these bacteria were
often mistaken for viruses because of their small size and
lack of walls. However, they do have other features of prokaryotic cells, such as prokaryotic ribosomes

First Time Here?

Welcome to Quizlet, a fun, free place to study. Try these flashcards, find others to study, or make your own.

Set Champions

There are no high scores or champions for this set yet. You can sign up or log in to be the first!