MCB2010 Test 2

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Created by:

sgoingundern  on June 2, 2012

Subjects:

Microbiology

Description:

Valentine Negrouk MDC MicroBio Test 2

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MCB2010 Test 2

Gene
The fundamental, physical, and functional unit of heredity; It is because a gene is a segment of DNA (on a specific site on a chromosome) that is responsible for the physical and inheritable characteristics or phenotype of an organism.
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Terms

Definitions

Gene The fundamental, physical, and functional unit of heredity; It is because a gene is a segment of DNA (on a specific site on a chromosome) that is responsible for the physical and inheritable characteristics or phenotype of an organism.
Transcription DNA to RNA
Translation RNA to Protein; mRNA, tRNA, riboRNA together.
Replication (genetics) the process whereby DNA makes a copy of itself before cell division
Complementary An adjective used to describe two bases that always pair up together (e.g. A and T, C and G)
DNA vs RNA RNA contains ribose instead of deoxyribose, RNA contains uracil instead of thymine like DNA, RNA is single-stranded rather than double-stranded like DNA, RNA is smaller than DNA
DNA Backbone nucleotides covalently bond to form a sugar-phosphate linkage; have a 5' carbon and a 3' carbon
DNA Strands - DNA is made up of two antiparallel strands joined by H-bond
DNA Components a nitrogenous base, a penose sugar called deoxyribose, and a phosphate group
DNA Harddrive of any system.
DNA Polymerase enzyme involved in DNA replication that joins individual nucleotides to produce a DNA molecule
RNA Polymerase an enzyme that pries the 2 strands of DNA apart and hooks together the RNA nucleotides as they base pair along the DNA template
Primary Structure the simplest of protein structures, the unique sequencing of amino acids
DNA Ligase A linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of a new DNA fragment to the 5' end of a growing chain
Restriction Enzyme enzyme that cuts sugar-phosphate bonds in the DNA backbone at specific points within particular nucleotide sequences in DNA
tRNA type of RNA molecule that transfers amino acids to ribosomes during protein synthesis
mRNA the template for protein synthesis
rRNA The most abundant type of RNA, which together with proteins froms the structure of ribosomes. Ribosomes coordinate the sequential coupling of tRNA molecules to mRNA codons; also called ribosomal RNA.
Nucleotide Sequencing 5' -> 3'
Polypeptide a peptide containing 10 to more than 100 amino acids
Start Codon AUG- as well as the codon for the amino acid methionine (therefore all proteins start with the amino acid methionine.)
Stop Codons UAA, UAG, UGA
Bacterial Cells Optimal conditions of 37 degrees celsius divide fast every 20 minutes.
Coding Regions sequences of DNA that serve as instructions for making proteins
Spacers DNA Sequences not of importance.
Mutagenesis Chemicals or radiation: used to create base pair substitutions or deletions. Technical details: expose mother to chemical/radiation-see what happens. Requires generating and screening a lot of organisms/phenotypes. Imperfect and random. Better techniques developed.
Recombinant DNA A DNA molecule made in vitro with segments from different sources.
Prokaryotic DNA simple; 1 chromosome; circular dna attached at both ends found in cytosol.
Eukaryotic DNA linear strands of DNA inside nucleus.

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