What is the function of staining in microscopy
to increase the contrast of the specimen AND to assist in classification of the specimen
For which size of specimen would a microscope be required?
Which of the following statements is true regarding electron microscopy?
Stains are used to enhance contrast during electron microscopy.
Light microscopy can view speciman
1mm - 200nm
Most common form of light microscopy is
The specimen to be observed rests on ____________.
What is the role of the condenser lens?
to focus the light to a high intensity in a small area
Which of the following does the bulk of the magnification of the specimen?
What type of image would you see with a transmission electron microscope?
a two-dimensional image of ribosomes
Which wavelength would result in the highest resolution?
The condenser lens of an electron microscope _____________________.
is an electromagnet
Which of the following are characteristics shared by both light microscopes and electron microscopes?
condenser and objective lens
3 main categories of stains used by Microbiologist
Simple, Differential, Special
Crystal violet purple stain
stains most bacteria uniformly
safranin a red dye
stains most bacteria pink or red
stains most bacteria light blue
most stains used in Microbiology are
Gram stain is most frequently used to _____
determine between Gram positive and Gram negative
Gram positive has a _____
thick outter cell wall composed of peptydoglycan
Gram negative has a _____
thin outter cell wall
Gram staining requires 7 steps
Crystal violet, rinse, mordant, rinse, decolorizing agent, counterstain safarnin, and rinse
stained a deep purple
stained pink by safranin