Microbiology test questions

86 terms by LINA12345 

Create a new folder

Advertisement Upgrade to remove ads

How do we define microorganisms in terms of size?

Cells up to 1mm

How many domains of life are there?

Three

Who proposed the binomial system of classification?

Carolus Linnaeus

Which of the following is a correct example of binomial nomenclature?

Streptococcus pyogenes (italized)

Which is the theory that states all living things are composed of cells?

Cell theory

Which theory opposed the theory of spontaneous generation?

Biogenesis

Who provided a method of demonstrating that a microorganism could be responsible for a particular disease? His/Her name is associated with a series of 'postulates'.

Robert Koch

We generally refer to microorganisms using binomial nomenclature. These names generally have two parts which refer to the..

Genus and Species

What does the bacterial species Bacillus thuringiensis produce?

Insecticide crystals

What is the smallest unit of matter that enters into a chemical reaction?

Atom

What do elements share when they create a covalent bond?

Electrons

How many atoms are in 1 mole of an element?

6.02 x 10 to the 23rd

What charge is associated with an electron?

negative

Which element has the atomic number 6?

Carbon

Which of these is the result of an interaction between ions?

NaCl

What does the pH scale describe?

How many H30+ ions are in a solution

Which molecules make up proteins?

Amino Acids

How many hydrogen bonds are there between adjacent G and C bases in DNA?

3

Which of these represents an amino (or amine) group?

NH2

In Figure 1 (next page), what is the total magnification of bacteria if you used the 100x objective and eyepiece of 10x?

1000x

The resolution of a light microscope can be improved by changing the...

Wavelength of light

Which microscope would let you best see a 300nm virus?

Electron microscope

In microscopy, what does the abbreviation SEM mean?

Scanning Electron Microscope

1000nm is equal to?

1um

TEM is the correct abbreviation for?

Transmission electron microscope

Which of the following pairs is mismatched?

Ribosome:energy storage

Look at figure 2, above. This shows the structure of , which is a thin structure lying inside the cell wall and enclosing the cytoplasm of the cell. It consists primarily of phospholipids and proteins

Plasma membrane

Look at figure 2, above. The phospholipid and protein molecules in the structure are not static but move freely in the structure surface. The structure must be as viscous as olive oil, so the proteins can move freely enough to function without destroying the structure. This arrangement of phospholipids and proteins is referred to as the ?

Fluid mosaic model

The , is also known as the capsule, slime layer, or extracellular polysaccharide, is a polysaccharide and/or polypeptide outside the cell

Glycocalyx

In Figure 3, which diagram represents a Gram negative cell wall?

A the one with the smaller peptidoglycan wall

Which number and unit would you associate with prokaryotic ribosomes?

16s

Which of the following is true?

Eukaryotes have histones bound to DNA and bacteria do not

Large molecules cross the cell membrane by?

Active transport

Peptidoglycan is only found in

The cell wall of most Bacteria

Chemotaxis describes the ability of bacteria to do what?

To move along a chemical gradient

Which term would you use to describe a cigar-shaped cell with rounded ends?

Rod

What is the function of flagella in microorganisms?

Motility

What is a major component of the Gram positive cell wall?

Peptidoglycan

The Archae cytoplamic membrane contains:

Isoprenoids

What is catabolism?

The breakdown of organic molecules

What is a competitive inhibitor?

A molecule that competes with the proper substrate for the active site

What is ATP?

Adenosine triphosphate

To what does the 'optimum temperature' of an enzyme refer?

The temperature at which the enzyme functions fastest

When a molecule is oxidized , it...

Loses electrons

Indole is produced by some members of the Enterobacteriaceae when they break down...

Tryptophan

In a microbiological context, what is EPS?

Extracellular Polymetric Substance

What are the four bases (or nucleotides) in a DNA molecule

GCAT

How many ATP molecules are produced in glycolysis?

2

What is made in the ribsomes?

proteins

Gram staining of bacteria enables one to distinguish two kinds of bacterial

cell wall

All prokaryotes lack which of these, if any:

Mitochondria

All life can be classified into these three domains:

Bacteria, Archaea, Eucarya

Bacteria are separated on the basis of the Gram Stain. Which of these is NOT a possible response in Gram Stain?

Sporulated

Stapylococcus aureus, an important member of the human micro biome, forms characteristic morphologies which are:

Yellow colonies, Gram positive 'grape like clusters

Edward Jenner is famous for his work on

smallpox

Which of the following statements about endospores is are true?

Endospore allow a cell to survive poor environmental conditions

Which of these Spirochetes causes syphilis

Treponema pallidum

The three principle stages of cellular respiration are?

Glycolysis, Kreb's cycle, electron transport chain (or system)

Which stage of aerobic respiration produces the most ATP?

Electron transport chain (or system)

How many different amino acids occur in nature?

20

In figure 1 (next page), line 'a' points to the microscope's

ocular lens

Who is widely acknowledged for inventing the microscope?

Antonie von Leeuwenhoek

Why are hyperthermophiles limited to the deep sea?

Water there can reach temperatures above 100 C

Where would you expect to find psychophiles?

In sea-ice

How might primitive civilizations have preserved food by a method that relies on osmotic pressure?

Use of high salt concentration

Which group of molecules contains all of the following: Carbon, hydrogen, oxygen, nitrogen, sulfur

Proteins

Capnophiles require the presence of high concentrations of which gas:

Carbon Dioxide

In order to grow, Obligate barophiles require at least which one of these:

High pressure

What does the abbreviation EPS mean

Extracelluar Polymetric Substance

Phosphorus is an essential component of which of these molecules:

Nucleic acids

Beijerink, Van Niel and Kluyver belonged to which school:

The delft school

A culture medium...

is a solid or liquid formulation of nutrients to support microbial growth

What is the inoculum?

The sample that is placed into the medium

What is agar?

A polysaccharide derived from a marine alga

What is Peptone

a partially digested protein

How might we remove dissolved 02 from a liquid?

Sparge (bubble) with nitrogen gas, Boil it, Add thioglycolate

A biosafety level 1 laboratory requires:

No special precautions

Selective media...

Allow growth of fewer microbes that would grow on a general medium, Use specific chemical, pH, or salinity, Can be brought pre-prepared or produced in the lab, Are often used in clinical microbiology

In microbiological context, a colony is:

A population of cells arising from a single cell or spore, or from a group of attached cells

In microbiological context, CFU is an abbreviation of:

Colony forming unit

In microbiological context, MPN is an abbreviation of:

Most probable number

Heterotrophic bacterial growth is also described as:

Secondary production

The 'generation time' refers to:

The time required for a cell to divide and the population to numerically double

Lag phase refers to that period when

Cells adjust to a new environment

When measuring bacterial growth, measurement of which of these are directed methods:

Turbidity, Dry weight, Metabolic activity

The plate count is said to measure what?

The number of cells that are 'viable'

Please allow access to your computer’s microphone to use Voice Recording.

Having trouble? Click here for help.

We can’t access your microphone!

Click the icon above to update your browser permissions above and try again

Example:

Reload the page to try again!

Reload

Press Cmd-0 to reset your zoom

Press Ctrl-0 to reset your zoom

It looks like your browser might be zoomed in or out. Your browser needs to be zoomed to a normal size to record audio.

Please upgrade Flash or install Chrome
to use Voice Recording.

For more help, see our troubleshooting page.

Your microphone is muted

For help fixing this issue, see this FAQ.

Star this term

You can study starred terms together

NEW! Voice Recording

Create Set