What is the purpose of incubating the unopened plates?
To use as a contol group. They will not change so one would have enough to control to study off of.
If growth appears on the unopened plates what probably happend?
it was contaminated between the autoclave and time of incubation.
Why were the specific types of exposure chosen for this exercise?
To procide bateria for the growth and produce a reaction for the exercise
Why were you asked to incubate the blates at two different temperatures?
They will likely be affected differently at different temperatures depending on how the bateria feeds of the media
What is growth media?
nutrients that allow bateria to be isolated and cultured
Why do microbiologists need growth media?
to classify bateria as a prokaryote (no nucleus) we need to give the bacteria what it likes to eat.
What are the requirements of growth media?
-carbon, oxygen, nitrogen, hydrogen
What are the categories of media?
categories of media: physical states
solid, can be solidified
solid, cannot be solidified
categories of media: chemical composition
Defined: synthetic, know the componets
Complex: non-synthetic, dont know exact porportions
Growth means in micro:
^ in population no size
Slant tube provides
more surface area in more confined space
organic and inorganic compounds in an exact chemical formula
at least 1 ingredient that is not chemically definable.
complex organic substances such as blood, serum, hemoglobin, or special growth factors required by fastidious microbes
grows a broad range of usually non-synthetic microbes
6 types of culture media
An exapmple of selective media
same sample but change the Ph one will grow bateria and one will grow fungus.
ehh it's okay blood
most commonly used media (4)
-trypticase soy broth agar
-brain and heart infusion broth agar
-Liquid beef extract and peptone
Trypticase soy broth or agar
-beef soy extract
-Solid beef extract, peptone, agar
Brain and Heart infusion broth agar
-Beef, heart, brain extract
-Polysacharide from red algae
-The theory of being everywhere.
The 5 I's of Culturing Microbes
growing bateria to study
Colony forming unit
culture composed of cells arising from a single progenitor (CFU)
-Temp controlled chamber
-microbe multiplies and produces macroscopically observeable growth
-If one bacterial cell is separated and has space on nutrient surface it will grow a colony.
-Thick Leg Like shape
-looks like filliments sticking out from middle
-Shallow curves around the bateria
-long curves around the bacteria
-lots of circular motions around the bacteria
-leg like extentions from middle
-slightly higher than just convex
- looks like a boob with a nipple
-2 or more species