using harmless chemicals (derived from bovine serum albumin, avidin, biotin, and horseradish peroxidase)
4 True/False Questions
This technique has been adapted for diagnosis of many microbial agents, including → HIV, influenza virus, hepatitis viruses, and the bacteria responsible for Lyme disease and Chlamydia infection.
To visualize the binding of anti-human antisera to the wells, the substrate for the enzyme linked to the anti-human antibody is added. In a positive reaction, the bound enzyme-linked antibody will convert the colorless substrate to a colored product. If no antibody binding has occurred, the wells will remain colorless. → Substrate
Plastic microtiter wells are coated with protein antigens derived from the pathogen. The microtiter wells are blocked with an irrelevant protein (such as bovine serum albumin) to prevent non-specific binding of patients' antibodies. → Preparation
The principle of the ELISA involves four steps: → enzyme-linked immunosorbent assay