4 Written Questions

1. Substrate
2. technique is a rapid and accurate method for detection of serum antibodies against specific pathogens
3. HIV, influenza virus, hepatitis viruses, and the bacteria responsible for Lyme disease and Chlamydia infection.
4. Preparation

4 Multiple Choice Questions

1. ELISA,HIV
   1. This exercise will simulate an _______to detect ____infection
   2. This is a simulation using
   3. what does ELISA stand for
   4. The principle of the ELISA involves four steps:
2. Samples
   1. This technique has been adapted for diagnosis of many microbial agents, including
   2. The principle of the ELISA involves four steps:
   3. Plastic microtiter wells are coated with protein antigens derived from the pathogen. The microtiter wells are blocked with an irrelevant protein (such as bovine serum albumin) to prevent non-specific binding of patients' antibodies.
   4. The patients' serum samples are added to the microtiter wells and incubated. If the patient has antibodies reactive with the pathogen's antigens (a positive sample), they will bind to the well. If the serum is negative for antibodies reactive with the pathogen (a negative sample), no binding will occur. After incubation, the wells are washed to remove any unbound antibodies.
3. presence of serum antibodies against a specific pathogen indicates that the patient is currently infected with that pathogen
   1. what is ELIZA?
   2. This is a simulation using
   3. positive ELISA test
   4. what does ELISA stand for
4. enzyme-linked immunosorbent assay
   1. what is ELIZA?
   2. positive ELISA test
   3. This is a simulation using
   4. what does ELISA stand for

4 True/False Questions

1. To determine whether any of the patients' antibodies have bound to antigen, an enzyme-linked anti-human immunoglobulin is added to the wells. The wells are incubated with the anti-human sera, then washed to remove any unbound antibodies. → Detection

   True        False

2. Does this excersize involve actual HIV antigens or human serum? → Preparation,Samples,Detection,Substrate

   True        False

3. This is a simulation using → using harmless chemicals (derived from bovine serum albumin, avidin, biotin, and horseradish peroxidase)

   True        False

4. The principle of the ELISA involves four steps: → Preparation,Samples,Detection,Substrate

   True        False