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WHAT DOES POLYCHROMATOPHILIC MEAN?

LOVING MANY COLORS

WHAT GIVES AN RBC ITS COLOR?

HEMOGLOBIN

WHAT DOES THE TERM CENTRAL PALLOR MEAN?

CLEAR COLORLESS CENTER

A DEEP BLUE CYTOPLASM IS KNOWN AS?

BASOPHILIC

DESCRIBE THE COLOR CHANGES OF AN IMMATURE RBC TO A MATURE RBC....AND WHY DOES THIS HAPPEN?

COLOR INSIDE THE CYTOPLASM GOES FROM BLUE-PURPLE/LAVENDAR-RED. CYTOPLASM CHANGES COLOR BECAUSE YOU ARE SLOWLY ADDING AMOUNTS OF HEMOGLOBIN

THE LACK OF RBCs IN BLOOD IS KNOWN AS?

ANEMIA

WHAT IS HEMATOPOIESIS?

THE PRODUCTION OF ALL BLOOD CELLS INCLUDING PLATELETS

THIS HORMONE IS PRODUCED BY THE KIDNEY WHEN HYPOXIA IS PRESENT.

ERYTHROPOIETIN (EPO)

WHAT IS ANOTHER NAME FOR A STEM CELL?

PROGENITOR CELL

WHAT IS THE PPSC, AND WHAT DO THE LETTERS STAND FOR?

PLURIPOTENT STEM CELL, IT IS THE COMMON ANCESTOR CELL

WHAT IS THE % OF PRORUBRICYTE IN THE BONE MARROW?

3-4%

WHAT IS THE % OF METARUBRICYTES IN THE BONE MARROW?

57%

WHAT IS THE % OF RUBRICYTES AND RUBRIBLASTS IN THE BONE MARROW?

RUBRIBLASTS-1%
RUBRICYTE-35%

RBCs ARE PRODUCED IN THE BONE MARROW EXCEPT WHEN IN THE FETUS, RBCs ARE PRODUCED WHERE?

LIVER AND SPLEEN

WHAT ARE THE 2 TYPES OF BONE MARROW, AND WHY DOES IT CHANGE?

THERE IS RED AND YELLOW BONE MARROW. THE RED MARROW CHANGES INTO THE YELLOW BONE MARROW WHEN THE ANIMAL GETS OLDER, AND FAT CELLS GET ADDED GIVING THE MARROW ITS YELLOW COLOR.

A MATURE RBC DOES NOT HAVE A WHAT?

NUCLEUS

WHY DO RBCs HAVE A CENTRAL PALLOR?

BECAUSE OF THEIR BICONCAVE DISK SHAPE

WHAT IS THE NUCLEUS TO CYTOPLASM RATIO IN A RUBRIBLAST?

7:1

IF RETICULOCYTES ARE OUT IN CIRCULATION, WHAT IS THIS A SIGN OF?

REGENERATIVE ANEMIA

LIST THE 4 THINGS THAT OCCUR AS AN RBC MATURES...

1. THE SIZE OF THE RBC GOES FROM LARGE-SMALL
2.THE CELLS CYTOPLASMIC COLOR CHANGES FROM BLUE-PURPLE-RED
3.THE CHROMATIN CLUMPS WITHIN THE CYTOPLASM
4. THE NUCLEUS SHRINKS AND EVENTUALLY DISAPPEARS

IN WHAT STAGE IS A NUCLEOLUS PRESENT?

RUBRIBLAST

IN THIS STAGE A PERINUCLEAR CLEAR ZONE IS PRESENT

PRORUBRICYTE

AT THIS STAGE THIS CELL IS SOMETIMES CALLED AN NRBC AND WHAT DOES NRBC STAND FOR?

THE METARUBRICYTE IS SOMETIMES CALLED AN NRBC WHICH MEAN NUCLEATED RED BLOOD CELL

THE MOST IMMATURE STAGE IS WHAT, AND HOW IS IT RECOGNIZED?

THE RUBRIBLAST, AND IT HAS A NUCLEOLUS

THESE CELLS MAY BE SEEN IN CIRCULATION DUE TO A SUDDEN LOSS OR DESTRUCTION OF RBCs

METARUBRICYTE (1ST STAGE YOU ARE ABLE TO SEE IN CIRCULATION)

WHAT IS ERYTHROPOIESIS?

THE PRODUCTION OF RBCs

WHAT ARE THE STAGESOF RBC MATURATION IN ORDER FROM IMMATURE TO MATURE?

1.RUBRIBLAST
2.PRORUBRICYTE
3.RUBRICYTE
4.METARUBRICYTE
5.POLYCHROMATOPHIL/RETICULOCYTE
6. RBC

WHAT IS ANOTHER WORD THAT CAN BE USED TO DESCRIBE SMALLER?

PYKNOTIC

1.RUBRIBLAST

-MOST IMMATURE
-COARSELY GRANULAR CHROMATIN
-HAS A NUCLEOLUS
-% IN BONE MARROW 1%
-NUCLEUS TO CYTOPLASM RATIO IS 7:1

2.PRORUBRICYTE

-HAS PERINUCLEAR CLEAR ZONE (WHITE RING)
-% IN BONE MARROW 3-4%

3.RUBRICYTE

-% IN BONE MARROW 35%

4.METARUBRICYTE

-NUCLEUS CONTAINS DENSE CHROMATIN
-MAY BE SEEN IN CIRCULATION DUE TO SUDDEN LOSS OR DESTRUCTION
-IF SEEN IN LARGE #'S A CORRECTED WBC COUNT MAY BE NEEDED (SOMETIMES MISTAKEN FOR LYMPHOCYTE)
-CANNOT CARRY OXYGEN
-COMMONLY CALLED NRBCs
-% IN THE BONE MARROW 57%

5.POLYCHROMATOPHIL/
RETICULOCYTE

-NO NUCLEUS
-1ST STAGE THAT IS CAPEABLE OF CARRYING OXYGEN
-IF SEEN=REGENERATIVE ANEMIA=GOOD
BAD IF SEEN IN HORSES

WHEN RETICULOCYTES ARE SEEN IN CATTLE,SHEEP, AND GOATS WHAT DOES THAT MEAN?

THEY HAVE MARKED (SEVERE) ANEMIA

WHICH STAIN DO YOU USE TO STAIN POLYCHROMATOPHILS? RETICULOCYTES?

POLYCHROMATOPHIL-DIF QUIK
RETICULOCYTES-NEW METHELYNE BLUE

6.RBCs

-NO NUCLEUS
-HAVE A CENTRAL PALLOR DUE TO THEIR BICONCAVE DISK SHAPE

RBCs ARE WHAT % OF HEMOGLOBIN?

33.33%

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