microbiology
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21 terms
Terms | Definitions |
|---|---|
 compound light microscope | total magnification is 100x ability to destinguish between 2 points |
| phase contrast | allows mo's to be seen in natural alive state summation amplitude and wave length |
| fluorescence microscopy | glow dye to enhance the image of the specimen via ultraviolet light (direct fluorescent antibody principle) |
| indirect test | antibody |
| transmission electron microscope | electron beam passed thru thin sections of specimen then focused on to flourescent screen |
| transmission electrom microscope | can magnify objects 10,000 to 100,000 times-- used to see internal structure of mo |
| staining | 4 types |
| basic dyes | dye with positive chg attracted to neg charged mo's --methylene blue |
| acidic dyes | dye with neg chg repelled by neg charged mo's --eosin dye |
| simple stain | one colour |
| differential stain | see different coloured mo's -- gram stain |
| various bacterial morphology | shape, arrangment |
| cocci | shape is round arrangment is diplococci, lancet, tetrad chains and clusters |
| bacillus | shape is rod, arrangment is clubbed shape, square, curved, fusiform, pleomorphic |
| bacillus/ helicoidal, spiral | shape is curved rod |
| helicoldial | spirochetes use axial filaments for motility |
| helicoldial | use flagella for motility |
| gram stain | allows to see diff bacteria by light micorscopy |
| gram positive | stains blue, purple, can ovserve cocci/bacillus, |
| gram negative | stains red, pink can observe cocci/bacillus |
| steps in gram stain | crystal violet, iodine (mordant), alcohol, safranin |
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