Microbiology Lab Practical 1 Review

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Name three items of safety you are required to have in the Lab.

Lab coat, Safety Glasses, Gloves

What Bio Safety Level is our lab?

BSL-1

Resolution

Closest distance 2 objects can be and still appear as 2 objects

Brightfield compound microscope

shows dark objects in a bright field and is used most often

condensor

consists of several lenses that concentrate light on the slide by focusing it into a cone.

iris diaphragm

controls the angle and size of the cone of light; controls the amount of light

monocular microscope

this microscope has only one ocular lense

binocular microscope

this microscope has two ocular lenses.

coarse adjustment knob

used for focusing with the low-power objectives

fine adjustment knob

used for focusing with the high-power and oil immersion lenses.

field of vision

the area seen through a microscope

total magnification

objective lens x ocular lens

oil immersion lens

this lens has the highest magnification and must be used with immersion oil

ocular lens

this lens remagnifies the image formed by the objective lens

body tube

this part of the microscope transmits the image from the objective lens to the ocular lens

objective lens

this lens on the microscope magnifies the specimen

Condenser

this part of the microscope focuses the light through the specimen

diaphragm

this part of the microscope controls the amount of light entering the condenser

refractive index

the amount of light that bends

resolving power

wavelength of light used divided by 2x numerical aperture

parfocal

when a subject is in focus with one lens.

Colony

multiple bacteria that arises from a single bacteria cell

How do you pour a petri plate?

Melt the agar deep in water bath at 100C, cool agar deep to touch in 50C water bath, use quickly or it will solidify again, turn petri dish with lid on top, gently mix media and pour onto plate, swirl petri dish to ensure uniform coverage, then let cool until solid 10-15 minutes with lid ajar

Agar Slant

melted agar tilted in tube and solidified

Agar plates

Made fro melted agar deeps poured in petri dish; It has a large surface area to view and count colonies

Composition of Nutrient Agar

peptone, beef extract, salt, agar 1.5%

Composition of TSA (Tryptic soy agar)

peptone from casein, peptone from soymeal, sodium chloride, agar1.5%

Asceptic technique

established to keep bacteria, fungi, viruses, and other microbes under control while they are being studied.

innoculation

taking a sample of bacteria from one media and placing it in another media

Simple stain technique

uses only one stain and provides better visualization

bacillus

rod shape

coccus

circular shape

spirillium

spiral shape

differential stain technique

uses multiple stains to distinguish bacteria

gram positive

Purple color

gram negative

red in color

what color will Acid fast bacteria stain?

Red

What color will non-acid fast bacteria stain?

green

differential stain

staining method used to identify mycobacterium genus

If you were given an unknown culture in the lab, which biosafety level would you observe?

BSL-2 or the highest available in the lab

iris diaphragm

this part of the microscope is used to increase and decrease the light transmitted through the slide

mechanical stage

this part of the microscope holds the slide in place while on the microscope

agar liquefies at what temperature?

100C

If you are going to inoculate liquid media, and you chose to sample a site that is dry, what should you do before drawing the sterile swab across the surface to be tested?

dip the swab in distilled water

what are bacteria using for nutrient agar?

beef extract, peptones

the proper procedure for the sample staining of a bacterium is:

heat fix, stain, rinse off, blot

convex

a description of the elevation of a bacterial colony above the surface of an agar plate

entire

a description of the MARGIN of edge characteristics of growth on an agar plate

lobate

a description of the MARGIN of edge characteristics of growth on an agar plate

curled

a description of the MARGIN of edge characteristics of growth on an agar plate

pellicle

this term is used to describe the bacterial growth that completely covers the surface of a broth tube

flocculation

this term is used to describe the large masses of bacterial growth that is seen suspended within the mediu of a broth tube

Flat

a description of the elevation of a bacterial colony above the surface of an agar plate

raised

a description of the elevation of a bacterial colony above the surface of an agar plate

umbonate

a description of the elevation of a bacterial colony above the surface of an agar plate

undulate

a description of the MARGIN of edge characteristics of growth on an agar plate

entire

a description of the MARGIN of edge characteristics of growth on an agar plate

lobate

a description of the MARGIN of edge characteristics of growth on an agar plate

filamentous

a description of the MARGIN of edge characteristics of growth on an agar plate

safranin

this stain is used as the counterstain for the gram stain

sediment

microbial cells settle at the bottom of a broth tube

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