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1. BLI - Acquisition time per frame (s): 10 - 300

2. BLI - Form of energy used: Visible to infrared light (500-900 nm)

3. BLI - Main advantages: relative measure of cell viability or cell function

4. BLI - Main disadvantages: Low anatomic resolution

5. BLI - Molecular sensitivity (mol/l): 10^-13 through 10^-16*

6. BLI - Spatial resolution (mm): 3 - 10

7. BLI - Tissue penetration depth (mm): 1 - 10

8. CT - Acquisition time per frame (s): 1 - 300

9. CT - Form of energy used: X-rays (<140 keV)

10. CT - Main advantages: Morphological detection of tumurs

11. CT - Main disadvantages: Relatively poor soft-tissue contrast

12. CT - Molecular sensitivity (mol/l): not well characterized.

13. CT - Spatial resolution (mm): 0.03-0.4 (animal), 0.5-1 (clinical)

14. CT - Tissue penetration depth (mm): >300

15. FLI - Acquisition time per frame (s): 10 - 2,000

16. FLI - Form of energy used: Visible to infrared light (500-900 nm)

17. FLI - Main advantages: Multiple reporter wavelengths enables multiplex imaging

18. FLI - Main Disadvantages: Light <600 nm prone to attenuation

19. FLI - Molecular sensitivity (mol/l): 10^-9 through 10^-11*

20. FLI - Spatial resolution (mm): 2 - 10

21. FLI - Tissue penetration depth (mm): 1 - 20

22. MRI - Acquisition time per frame (s): 60 - 3,000

23. MRI - Form of energy used: Radio frequency waves (64 MHz)

24. MRI - Main advantages: anatomical detail and functional information

25. MRI - Main disadvantages: Low sensitivity, long acquisition

26. MRI - Molecular sensitivity (mol/l): 10^-3 through 10^-5

27. MRI - Spatial resolution (mm): 0.025-0.1 (animal), 0.2-?(clinical)

28. MRI - Tissue penetration depth (mm): >300

29. PET - Acquisition time per frame (s): 1 - 300

30. PET - Form of energy used: Annihilation photons (511 keV)

31. PET - Main advantages: High sensitivity;

32. PET - Main disadvantages: low resolution

33. PET - Molecular sensitivity (mol/l): 10^-11 through 10^-12

34. PET - Spatial resolution (mm): 1-4 (animal), 6-10 (clinical)

35. PET - Tissue penetration depth (mm): >300

36. SPECT - Acquisition time per frame (s): 60-2,000

37. SPECT - Form of energy used: Gamma rays (140keV)

38. SPECT - Main advantages: Multiple probes can be detected simultaneously;

39. SPECT - Main disadvantages: Between 10 and 100-fold less sensitive than PET

40. SPECT - Molecular sensitivity (mol/l): 10^-10 through 10^-11

41. SPECT - Spatial resolution (mm): 0.5-5 (animal), 7-15 (clinical)

42. SPECT - Tissue penetration depth (mm): >300

43. Ultrasound - Acquisition time per frame (s): 0.1-100

44. Ultrasound - Form of energy used: High-frequency sound waves (20 KHz)

45. Ultrasound - Main advantages: Images morphology and physiology of tissue relatively close to the surface of the mouse in real time

46. Ultrasound - Main disadvantages: Limited ability to image through bone or lung

47. Ultrasound - Molecular sensitivity (mol/l): not well characterized.

48. Ultrasound - Spatial resolution (mm): 0.05-0.5 (animal), 0.1-1 (clinical)

49. Ultrasound - Tissue penetration depth (mm): 1 - 200