5 Written questions
5 Matching questions
- Constitutive enzymes
- RNA polymerase
- Regulating the expression of genes
- a Enzyme that synthesizes RNA using single stranded DNA as a template; synthesis always occur in the 5' to 3' direction.
- b 1) Nucleotide sequence codes for regulation mechanism for gene expression 2) Mechanisms determine duration of synthesis of gene products -> Products are only made when required 3) Key mechanism is regulation of mRNA synthesis from DNA -> Regulation of transcription
- c Sequence of 3 nucleotides in a tRNA molecule that is complementary to a particular codon in mRNA. The anticodon allows the tRNA to recognize and bind to the appropriate codon.
- d Constantly synthesized -> Enzymes of glycolysis
- e Adenine
5 Multiple choice questions
- 1) Involves two separate but interrelated process, transcription and translation 2) RNA polymerase binds to a region of the DNA called the promoter 2.1) Only one strand of DNA acts as a template 2.1.1) This is called the sense strand 2.1.2) The strand not transcribed is the nonsense strand 3) Nucleotides in RNA are the same as those in DNA with one exception 3.1) Thymine is replaced with uracil 3.1.1) Binding in RNA is A:U or U:A and C:G or G:C 4) RNA polymerase continues down strand of DNA until it reaches a site on DNA called the terminator -> At the terminator RNA polymerase and the new strand of mRNA are released from strand of DNA 5)
- Ribosomal RNA -> type of RNA molecule present in ribosomes. rRNA builds the ribosomes
- 5'PO4. 1 strand of the DNA has a phosphate attached at the number 5 carbon of the sugar. -> Termed the five prime (5') end
- G -> C:G. G is bound to C by three hydrogen bond
- Process of synthesizing RNA from DNA template 1) mRNA carries the coded information from DNA to the ribosome, which is the site of protein synthesis 2) mRNA also plays an important role in translation 3) During transcription the enzyme, RNA polymerase, synthesizes a complementary strand of mRNA from a portion of unwound DNA
5 True/False questions
(C) → Cytosine
Start codon → Codon that terminates translation, signaling the end of the protein; there are 3 stop codons
DNA (double stranded molecule characteristic) → 1) Strands are complementary to each other -> Due to the specific base pairing of bases -> A:T and C:G 2) Strands are held together with hydrogen bonds -> A is bound to T by two hydrogen bonds and G is bound to C by three hydrogen bond
Melting or denaturing → Grouping of a stretch of nucleotides into sequential triplets; an mRNA molecule has 3 reading frames, but only 1 is typically used in translation.
Central dogma of molecular biology → Flow of information from DNA to RNA to protein