5 Written questions
5 Matching questions
- x-ray crystallography
- origin of replication
- template strand
- DNA ligase
- a A linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of one DNA fragment (such as an Okazaki fragment) to the 5' end of another DNA fragment (such as a growing DNA chain).
- b The DNA strand that provides the pattern, or template, for ordering the sequence of nucleotides in an RNA transcript.
- c A technique that depends on the diffraction of an X-ray beam by the individual atoms of a crystallized molecule to study the three-dimensional structure of the molecule.
- d Site where the replication of a DNA molecule begins, consisting of a specific sequence of nucleotides.
- e One of two types of nitrogenous bases found in nucleotides, characterized by a six-membered ring. Cytosine (C), thymine (T), and uracil (U) are pyrimidines.
5 Multiple choice questions
- A virus that infects bacteria; also called a bacteriophage.
- The tandemly repetitive DNA at the end of a eukaryotic chromosome's DNA molecule that protects the organism's genes from being eroded during successive rounds of replication. See also repetitive DNA.
- (1) The conversion of a normal animal cell to a cancerous cell. (2) A change in genotype and phenotype due to the assimilation of external DNA by a cell.
- Nucleotide sequences, usually noncoding, that are present in many copies in a eukaryotic genome. The repeated units may be short and arranged tandemly (in series) or long and dispersed in the genome.
- Eukaryotic chromatin that remains highly compacted during interphase and is generally not transcribed.
5 True/False questions
Single-strand DNA-binding protein (SSBPs) → A protein that binds to the unpaired DNA strands during DNA replication, stabilizing them and holding them apart while they serve as templates for the synthesis of complementary strands of DNA.
Okazaki fragment → A short segment of DNA synthesized away from the replication fork on a template strand during DNA replication, many of which are joined together to make up the lagging strand of newly synthesized DNA.
Lagging strand → The new complementary DNA strand synthesized continuously along the template strand toward the replication fork in the mandatory 5'?3' direction.
primer → An enzyme that joins RNA nucleotides to make the primer using the parental DNA strand as a template.
radioactive isotope → An isotope (an atomic form of a chemical element) that is unstable; the nucleus decays spontaneously, giving off detectable particles and energy.