Advertisement Upgrade to remove ads

Based on "Clinical Laboratory Science Review: A Bottom Line Approach" 4th Ed.

Which of the following is the correct temperature at which an autoclave must be run?

-37*C
-121*C
-25*C

121*C

How long is an autoclave run?

15 mins

How many lbs per square inch of pressure is necessary when using an autoclave?

15 lbs/sq in

Which of the following is the correct depth and pH of a Mueller Hinton plate?

-6mm and pH 7.2-7.4
-3mm and pH 6.5-6.8
-4mm and 7.2-7.4

4mm and pH 7.2-7.4

What is the McFarland standard for use in the Kirby Bauer method?

0.5 (10^8 organisms)

Which combination of organisms are used as QC for susceptibility testing using the Kirby Bauer method?

-S. pyogenes, P. mirabilis, N. lactamica
-P. vulgaris, S.pyogenes
-E. coli, S.aureus, P. aeruginosa

E. coli, S.aureus, P. aeruginosa

Which anaerobic bacteria is isolated on Bacteroides Bile Esculin Agar (BBE)? What color do the colonies appear as?

-B. fragilis, black colonies
-C. difficile, green colonies
-B. pertussis, yellow colonies

B. fragilis, black colonies

Kanamycin-Vancomycin Laked Blood is used to isolated which anaerobic bacteria?

-Clostridium species
-Legionella species
-Bacteroides species

Bacteroides species

Cycloserine-Cefoxitin Fructose Agar (CCFA) is used to isolate which anaerobic bacteria?

-Mycobacteria species
-C. diptheriae
-C. difficile

C. difficile

What factors is CDC Anaerobic Blood Agar enriched with?

-hemin, cysteine, and vitamin K
-X, V, and hemin
-hemoglobin, X, and V

Hemin, cysteine, and vitamin K

Cooked and or chopped meat is used to aid in the isolation of what type of bacteria?

anaerobes

Bordet-gengou agar isolates which of the following?

-B. fragilis
-B. pertussis
-F. tularensis

B. pertussis

Which media is used to isolate Legionella species?

Buffered Charcoal Yeast Extract

Which media is used to isolate F. tularensis?

Cystine-Glucose Agar

Skirrow agar isolates what species of bacteria?

Helicobacter pylori

Fletcher's medium isolates what bacteria?

Leptospira

Thiosulfate Citrate-Bile Salts (TCBS) is used to isolated which bacteria?

Vibrio species

Vaginalis Agar (human blood) is used to isolated which bacteria?

Gardnerella vaginalis

Both Cystine-Tellurite Blood and Loeffler's medium can be used to isolate which of the following species of Clostridium?

-difficile
-diptheriae
-tetani

C. diptheriae (black colonies)

What is the correct blood to broth dilution when perfoming a blood culture?

- 1:5
- 1:20
- 1:10

1:10

When is the best time to draw a blood culture?

- during a fever spike
- just prior to a fever spike
- just after a fever spike

just prior to a fever spike

What must be used to prep the skin prior to drawing a blood culture?

70% alcohol and iodine

What is the max # of blood culures that may be drawn within a 24 hour period?

3

What is the drug of choice for MRSA?

Vancomycin

S. aureus is most resistant to which antiboitic?

penicillin

Which gram postive cocci appears on a plate as mucoid "water drop" colonies, is sensitive to optochin and is bile soluble?

S. pneumoniae

Which of the following would be the correct identifying biochemical test reaction which would differentiate Citrobacter sp. from similar organisms?

VP positive
Lysine negative
Motility negative

Lysine negative

What would be the correct identifying biochemical test reaction(s) which would differentiate Enterobacter sp. from similar organisms?

VP positive, DNAase negative, Ornithine positive

Which of the following would be the correct identifying biochemical test reaction(s) which would differentiate Escherichia sp. from similar organisms?

VP negative
Urease positive
Lysine negative

VP negative

Which of the following would be the correct identifying biochemical test reaction(s) which would differentiate Morganella sp., Proteus sp., and Providencia sp. from similar organisms?

Lysine positive
Deaminase positive
Motility negative

Deaminase positive

Which of the following would be the correct identifying biochemical test reaction(s) which would differentiate Salmonella sp. from similar organisms?

VP positive
Urease positive
Lysine positive

Lysine positive

What is the break down product of Lysine?

Cadaverine

What is the break down product of Ornithine and Arginine?

Putrescine

What is the color of a positive Nitrate, Indole, Urease, and VP test?

Pink

You are performing a biochemical test on an isolated colony of bacteria. You perform an oxidase test, what color would a positive reaction be?

Purple

You perform an ONPG test and the reaction color is yellow. Is your result positve or negative?

Positive

If you perform a phenylalanine deaminase test and your reaction yields a green color, what is your interpretation of the reaction?

Positive

You have an organism that has an A/A, gas producing TSI reaction and is H2S positive, Phe deaminase negative, LDC (Lysine decarboxylation) negative. Which bacteria is most likely present?

Citrobacter sp.

You have an organism that has an A/A, gas producing TSI reaction and is H2S positive, Phe deaminase positive, and Indole positive. Which bacteria is most likely present?

Proteus vulgaris

You have an organism that has an A/A, gas producing TSI reaction and is H2S negative, Indole negative, LDC (Lysine decarboxylase) positive, and ODC (Oxalate decarboxylase) negative. What is the most likely organism?

Klebsiella sp.

You have an organism that has an A/A, gas producing TSI reaction and is H2S negative, Indole positive, and Citrate negative. What is the most likely organism?

E. coli

You have a K/A, H2S producing TSI reaction with biochemical reactions of Phe deaminase negative, Indole negative, LDC (Lysine decarboxylase) positive, and ODC (Oxalate decarboxylase) negative. What is the most likely identification of the organism?

Salmonella typhi

Which gram negative non fermenting bacteria are associated with cystic fibrosis patients?

Burkholderia cepacia and Pseudmonas aeruginosa

Haemophilus influenzae requires what two growth factors?

X (hemin) and V (NAD)

What test is used to determine sensititvity to ampiciliin when dealing with Haemophilus influenzae?

Beta lactamase

You receive a sputim sample from a patient who has recently returned from a vacation at a lake. The organism grows on BCYE (Buffered charcoal yeast agar). Which bacteria is present?

Legionella sp.

What organism is associated with Pseudomembranous Colitis (PMC)?

Clostridium dificile

Which gram positive anaerobe is responsible for causing "Lumpy jaw"?

Actinomyces israeli

Which gram positive anaerobe is associated with sulphur granules?

Actinomyces israeli

What is the cause and vector responsible for Lyme disease?

Borrelia burgdorferi/Ixodes (deer/mouse) tick

What is the proper procedure to culture and isolate Borrelia burgdorferi?

Culture in Kelly medium at 33C - examining in darkfield microscopy weekly for one month

Which obligate intracellular parasite is identified by a Giemsa stain and growth in yolk sac of chick embryo or tissue culture (McCoy cell)?

Chlamydia sp.

How many Mycobacteria must be present on a smear to be considered significant?

Any number
>20
>50

Any number

In order for a sputum specimen to be considered acceptable when processing for Mycobacteria, what type of collection must it be?

First morning, 3 consecutive mornings

If a specimen suspected of Mycobacteria contamination was received in a non sterile container, would this be criteria for rejection?

Yes

Must a specimen for Mycobacteria isolation be collected sterily?

No (the CONTAINER must be sterile)

What chemical is used to decontaminate a Mycobacteria specimen?

NaOH

Which species of Mycobacteria are photochromogen pigment producers?

Mycobacterium marinum and mycobacterium kansasii

Which species of Mycobacterium are scotochoromogens?

Mycobacterium scrofulaceum and mycobacterium gordonae

Which species of Mycobacterium are non photochromogens?

Mycobacterium avium and mycobacterium ulcerans

If gram positive cocci are identified from a sputum sample, what are the most likely type(s) of bacteria?

Staphylococcus aureus and streptococcus pneumoniae

What bacteria would most likely be associated with peptic ulcers?

Heliobacter pylori

What colony count indicates a UTI?

>100,000 organisms/mL

How does transport media differ from that of bacteria media?

Contains nutrients (fetal calf serum or albumin) and antibiotics

Describe the DNA probe method.

Molecular cloning of a specific DNA sequence

Describe the PCR method.

Method in which nucleic acid sequences are amplified in vitro. (Each cycle produces double the amount of desired nucleic products)

Which RNA virus causes Yellow Fever (aka Dengue fever, St. Louis encephalitis)?

Flavivirus

Which RNA virus causes Pulmonary syndrome/Hemorrhagic fever?

Hantavirus

Which RNA virus causes Measles?

Morbilivirus

Which RNA virus causes Polio myelitis/Aseptic meningitis?

Poliovirus

Which RNA virus causes Rabies?

Rhabdovirus

Which RNA virus causes Acute Infectious Infantile Diarreha?

Rotavirus

Which RNA virus causes Rubella?

Rubivirus

Which human T-cell leukemia virus is responsible for causing Hairy Cell leukemia?

HTLV II

Which virus is associated with Negri bodies in brain tissue of infected animals?

Rhabdovirus

Which DNA virus causes respiratory infections?

Adenovirus

Which DNA virus causes mental retardation?

Cytomegalovirus

Which DNA virus causes infectious mononucleosis?

Epstein Barr virus

Which DNA virus causes oral/genital infections?

Herpes simplex virus I and II

Which DNA virus causes a cervical carcinoma?

Human Papillomavirus (HPV)

Which DNA virus causes smallpox?

Poxvirus

Which DNA virus causes chickenpox/shingles?

Varicella-zoster virus

Please allow access to your computer’s microphone to use Voice Recording.

Having trouble? Click here for help.

We can’t access your microphone!

Click the icon above to update your browser permissions above and try again

Example:

Reload the page to try again!

Reload

Press Cmd-0 to reset your zoom

Press Ctrl-0 to reset your zoom

It looks like your browser might be zoomed in or out. Your browser needs to be zoomed to a normal size to record audio.

Please upgrade Flash or install Chrome
to use Voice Recording.

For more help, see our troubleshooting page.

Your microphone is muted

For help fixing this issue, see this FAQ.

Star this term

You can study starred terms together

NEW! Voice Recording

Create Set