B. Subtilis in Startch Hydrolosis
there was a clearing around growth once the iodine was applied and is positive for amylase present
S. aureus in starch hydrolosis
there was no clearing around growth once the iodine was applied and is considered negative for amylase
Casease Test requires what kind of Agar plate, and why?
TSA milk agar plate because it has protiens and we want to see if the bacteria is able to break down protiens (casein)
What is the enzyme some bacteria produce to hydrolyze the protien?
E.coli in the Casease test
had no clearing in the agar; casease was not present
B. Sbutilis in the Casease test
had a present clearing in the agar; casease was present
the Gelatinase test is to test what?
to see if the organisim secretes gelatinases - which liquefys the nutriant gelatin
how do you preform the Fluid Thioglycollate Medium test?
you stab the nutrient agar with the organism and then inoculate it
What is E. coli in the Gelatinase test?
it is negitive for gelatinase ; it was still a solid
What is S.marcescens in the Gelatinase test?
it was positive for gelatinase; gelatin is liquid
What test is this? Which is considered positive?
It is a Gelatinase test; Top is positve b/c it is liquid
an enzyme that catalyzes the depolymerization fo DNA into small fragments
Purpose of DNase test?
is to see if the bacteria has the ability to produce the DNase enzyme
S. Aureaus in DNase test
is negative; it has no clearing or growth
S. epidermidis in DNase test
was positive ; clearing in agar around growth
What does the Lipase test look for?
if the bacteria contains the enzyme that hydrolyze fats
lipases are what?
the enzymes that hydrolyze fats
S. aureus in Lipase test?
is negative ; it does not have a clearing around growth
E. coli in Lipase test?
is positive and does have a clearing around growth
Phenol Red (PR) broth test is used to determine what?
acid and gas production with the bacteria
If a broth is yellow and has no bubble?
contains fermentation with acid and gas - A/G symbol
If a broth is yellow and no bubble?
cotains fermentation with acid but no gas- A/- symbol
If broth is red and has no bubble?
no fermentation - -/- symbol
if broth is pink broth and has no bubble?
Degradation of peptone; alkaline - K symbol
E. Coli in PR test?
Glucose- yellow and no bubbles - A/G; Lactose -yellow and no bubbles- A/G; Sucrose- pink and no bubbles- K
P.Vulgaris in PR test?
Glucose-yellow and no bubbles- A/G; Lactose-pink and no bubbles-K ; Sucrose- yellow with no bubbles- A/G
A. faecalis in PR test?
Glucose, Lactose, and Surcrose-all red with no bubbles- -/-
the MR test is to determine what?
to see if a bacteria contains mixed acid fermentation
MR test stands for what?
Methyl Red test
VP test is to determine what?
to see if the bacteria has butanediol
VP test stands for what?
the Voges-Proskauer test
what is added to determine if the bacteria is MR?
What is added to determine if the bacteria is VP?
15 drops of Reagent- A ; 5 drops of Reagent- B
E.Coli in MR test was ?
positive; MR turned red immediately
E. aerogenes in MR test was?
negitive; It did not change color
E. areogenes in VP test was
positive red in about 60 mins. red on top
E. coli in VP test was
negetive no color change; yucky blackish color on top
what is the Fluid Thioglycollate Medium test used for?
to dertermine the oxygen tolerance of the bacteria
In the Fluid Thiogycollat Medium what is C. Sporogenes?
Obligate aerobes will only grow in this oxygen-rich top layer
In the Fluid Thiogycollat Medium what is P. aeroginesa?
will only grow in this oxygen-rich top layer; strict anerobic
In the Fluid Thiogycollat Medium what is S. aureus?
will grow all over
Anerobic Jar results with C. Sporogenes?
it only grew in the jar as anaerobic only
Anerobic Jar results with P. aeroginesa?
it only grew on the plate as aerobic only
Anerobic Jar results with S. aureus?
it is faculatative and grew in both the jar without air and a plate with air
The catalase test is used to determine what?
to see if the organism produces the enxyme catalase
S. epidermis is was what in the Catalase test?
positive; it showed bubbles when hydrogen peroxide was added
E.faecalis was what in the Catalase test?
no bubbles when the hydrogen peroxide was added
The oxidiase test is for what?
to identify if the bacteria contains the respiratory enzyme cytochrome c oxidase
E. coli was what in the oxidiase test?
it was negative and turned a brownish color
P. Aerugniosa was what in the oxidiase?
it was positive and turned purple after the oxided water is added
The enzyme we are looking for in the starch hydrolysis test is
what reagent is added to see the positive in starch hydrolysis test
The MR-VP tests are part of the ___________________ battery of tests to separate the coliforms into two two categories for further testing and identification.