Producing Monoclonal Antibodies

Created by bambinana 

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antibodies

Selectively react with specific molecules or antigens and provide useful reagents for detecting molecules of interest

host animal

Antibodies are generated by immunizing a ~ with a foreign molecule or antigen

B lymphocytes

Cells that recognize and respond to antigens

epitope

A localized region on the surface of an antigen that is chemically recongized by antibodies; also called antigenic determinant.

polyclonal

Antibodies that consist of a variety of antibodies that recognize different sites on the antigenic molecule

monoclonal

Antibodies that recognize a single, specific epitope of an antigen of interest

test bleed

An examination of blood samples to see if the antibodies of interest exist

spleen

If the desired antibodies are being produced, the ~ is removed from the host and put in culture medium

myeloma cells

Included in culture medium and can divide indefinitely but do not produce antibodies

polyethylene glycol

PEG stands for

hybridomas

When PEG is added to the culture medium, some myeloma cells and antibodies fuse to form

die

Unfused B cells will eventually

dissociated

Unfused myeloma cells and hybridomas need to be

HAT

Special medium in which unfused myeloma cells die

Clonal cultures

Wells in which all of the cells are clonal (i.e. identical) because they are all derived from a single cell

Screening

A step during which it is identified whether the desirable cell has been produced or not

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