What does the decarboxylation test for?
to see which bacteria can decarboxitate and thus produce the decarboxylase enzyme
What is added after inoculaton to encourage anerobic conditions in decarboxylation test?
a small amount of mineral oil
What are the three decarboxylase media in the decarboxylation test ?
arginine,lysine, and ornithine
What is an example of a positive bacteria, and what is the ph level and color in the decarboxylation test?
The positive bacteria is purple with a PH level of 6.8 or above. E. Aerogenes was positive in both ornithine and lysine.
what is an example of a negative bacteria, and what is the ph level and color in the darboxylation test?
P. vulgarius is a negative bacteria that turned yellow. It has a PH level of 5.2 or below.
What is the purpose of the Citrate test?
to see wether or not a bacteria is utilizing citrate as a carbon source
What ph indicator is added to the citrate test?
Bromthymol blue dye is added to indicate the positive and negative ph factor.
What is an example of a postive bacteria in the citrate test, and what is the positive indicator and ph level?
E. aerogenes was a positive bacteria because when bromthymol blue dye was added it turned blue which indicated a ph level of 7.6
What is an example of a negatvie bacteria in the citrate test , and what was the ph level?
E. coli was negative because it stayed green one the byonthymol blue dye was added and the ph level was 6.9 or less
what is determined in the phenylanine deaminase test?
to see if there is phenylpyruvic acid is produced and deamination is present
what is added to the phenylanine deaminase test indicate a postitive reaction?
which bacteria is negative in the phenylanine deaminase test?
E. coli was negative and was yellow
which bacteria was positive in the phenylanine deaminase test?
P.Vulgaris was positive and was green
what does the Urease test determine?
to see if the bacteria produces the enzyme urease to break down urea
What common problem can be determined from the Urease test?
urinary tract infections
What is a positive bacteria in the urease test?
P. Vulgaris was hot pink and was positive and have a Ph level of 8.4 or above
what is negative bacteria in the urease test?
E.coli was negative and was orange and had a ph level under 8.4
what kind of test is the SIM test and what does SIM stand for?
a combination differential media, and it stands for Sulfur Indole motility test
What is the purpose of the SIM test?
to determine if the bacteria can produce sulfur reduction, indole production from tryphtophan, and motility.
what two enezymes can show sulfur reduction, and what indicating factor will make it positive in the SIM test? and what is an example of a positive bacteria?
cysteine desulfurase and thiosulfate reductase enezyme. The positive bacteria will be black. E.col and Salmonella typhin were both positive for sulfur reduction
How was motlity determined in SIM test, and which bacteria was positive?
Each bacteria was stabbed with a needle before inoculation and cloudiness or spread will determine mobility. Salmonella typhin was positive for mobility
The Indole test in the SIM test is to determine what enzyme? And what is added to deterimine a positive reaction?
the typtopanse enzyme. The reagaent Kovac is added, and turns the indoline bacteria red.
What kind of test is the TSI test? and what does TSI mean?
differential media test; triple sugar iron agar test
what does the TSI test ( test ) for?
glucose, lactose, and sucrose fermentation and sulfur reduction
does the medium show anaerobic or aerobic growth enviroments?
what are the results of the TSI test and what bacteria was tested in this test?
A.faecalis = pink all through no fermentation K/K ; E.coli = yellow and positive for all sugar fermation A/A; S.typhim= glucose fermation with reversion K/A (red slant &yellow but) and h2s (sulfur reduction); Proteus vulgarius = A/A positive for all sugar fermation and h2s (sufur reduction)
what is the TSI test presumtive for?
what is the MSA test and what does it test for?
it is a slective and differential test that determines or isolates staphylococci because it can only withstand the amount of salinity.
what is selective and what is differential in the MSA test?
manitol salt is selective and the mannifal fermentation is differential
what is postive and what is negative in the MSA test and why?
both S. Aureus and S.epidermidis are both grow with salt ( halofies) only S. aureus allowed the fermantion which turned yellow and indicated a positive reaction and S. epidermidis pink which made it negative
what is the PEA test and what does it test for?
it is a strictly selective test to find only gram + ( inhibits gram - organisms) . It stands for Phenyl Ethyl Alcohol test. Isolates steptococci and staphylococci
how does the PEA test interfer with gram - organisims
phenylethyl alcohol interfers with the DNA synthesis of gram - organsims
Which organisms were postive and negative in the PEA test?
E.coli is gram - and was negative in this test. S.aureus is positive and E. faecalis was also positve ( related to strep)
the coagulase test determines the difference between what?
this test is the determine S.aureus from other gram positive cocci.
how is the coagulase test determine what is positive?
if there is clumping it is positve and is considered the gold standard
what kind of test is the Blood agar test?
it its a differential media to determine hemolysis
give an example of a positve bacteria in the blood agar ?
S. pyogenes wasa positve and showeda beta,clear and complete break down of the blood. Alpha is an incomplete breakdown and is a greenish color. and gamma is no hemolysis .
What does the 6.5% NaCl broth test determine?
to see if thebacteria can grow in salt
6.5 % NaCl broth test's postive shows what?
Turbity= cloudy (E.faecalis negative) and S. pyogenes (negative)
What does the Bile Esculin test , test for?
to determine that bacteria can grow in the presence of bile
What bacteria in the Bile Esculin test is positve and what indicates why?
E. faealis and S.bouis are both positive because they are at least half black. S. pyogenes is negative because it is brownish looking.
Bacitracin susceptibility test
to see how bugs resopnd to antibiotics and determine antibiotic sesptability
what are the two antibiotics used int bacitracin susceptibility test?
Optochin (p) and bacitracin (a )
which bacteria had a clearing for Optochin(p) but not bacitracin(a) ?
which bacteria has a clearing for bactracin(a) but not for Optochin(p)?
What do you need to look for in order to have a positve bacitracinsusceptibility test?
a killing zone around the antibiotic
the EMB test stands for what? What does it contain?
the Esin Methylene Test. It contains peptone, lactose, sucrose, and the dyes eosin Y and themethylene blue
what is the EMB test for?
the sugars provide fermentible substrates toencourage growth of fecal coliforms. This test weeds out the growth of gram positive ogranisms.
What bacteria was considered positive int EMB test?
E. aerogenes was the gram - organisim that turned purple; Ecoli was also gram - and turned a shiney green blackish color; S.typho is also gram - and was pink; E. faecili was negative with no growth because it is gram positive
What is the HE test?
Hektoen Entric Agar test; it is used to isolate and differentiate Salmonell and Shigella from other gram negative
What was considered gram negative and a lactose fermenter in the HE test?
What was considered a lactose fermenter and a sulfur reducer?
The MAC test is what? and what is it used for ?
it is the MacConkey Agar test; it is used to see if an organism can ferment lactose. It only allows gram positive orgranisms to grow.
What is the PH level of the indicator in the MAC test when it is red? and when it is neautral?
it is nueatral at 6.8 and above and red below 6.8
What were the results of the organisms in the MAC test?
E. Coli produces acid from lactose fermentation( A). S.typh is negative nothing grew (gram postive (P) and E. aergoenes had little growth P()