5 Written Questions
5 Matching Questions
- 99% of genes in our body are in fact encoded in our
- **Compare top-down selection on microbial community vs. bottom up
- Prevalent genes
- MetaHIT project analyzed this many base pairs of DNA- compared to what previous amount?
- Environmental genomics is also known as
- a genes from the most abundant reads
- b gut
- c Metagenomics
- d MetaHIT project studied 577 Gb, whereas previous metagenomic studies only did 3 Gb microbe DNA from fecal samples of 33 people
- e Top down is when selection pressure on host results in a microbial group selection in the gut as well as the host immune system and intermicrobial dynamics playing a role in the gut. Bottom up selection (99% of genes in our body are encoded in our gut) - genome interactions influence phenotype of cells, selection of cell phenotype results in selection of specific genomes
5 Multiple Choice Questions
- 1. Single gene - many microbes with mixed 16S sequences. 2. Metagenomics-sequencing many genes from many microbes
- 500 bp, 42.7%
- Bacteroides uniformis
- 577 Gb. 200 fold more sequence data than previous experiments. Allowed them to predict 3.3 million unique genes. There are only 25K genes in human genome - so there are 99% microbial genes, 1% human genes in our body.
- colon with 10^11 to 10^12 cells/ml
5 True/False Questions
We are born germ free but by adulthood - # abundant bacterial species in the gut → 1150
MetaHIT project - what was it? What did it set out to do? → 124 europeans - healthy, overweight, and irritable bowel syndrome
Minimal core genome is around # genes → Metagenomics
MetaHIT project found that there is a x repertoire that is likely required for proper gut functioning → consortium of 13 partners spanning 8 countries and 21.2 million Euros. Set out to fully analyze the gut genome.
This new type of sequencing uses reversible chain termination and chemoluminescence → Solexa sequencing