5 Written Questions

1. a way scientists can study whole sets of genes and their interactions
2. a gene carrier in gene cloning
3. the scientific analysis of eidence for crime scene and other legal investigations
4. the repetitive DNA at chromosome ends
5. very similar to the genome of a mouse

5 Matching Questions

1. the process of gene therapy
2. DNA technology
3. transposons
4. genetically modified (GM) organisms
5. recombinant DNA

1. a "jumping genes" DNA segments that can move or be copied from one location to another in a chromosome and even between chromosomes
2. b the normal gene is clond and then inserted into the nucleic acid of a retrovirus vecto that has been rendered harmless. Bone marrow cells are taken from the patient and infeted with the virus. the virus inserts its nucleic acid, including the human gene, into the cells' DNA. The engineered cells are then injected back into the patient.
3. c methods used to study and/or manipulate DNA, including recombinant DNA technology
4. d one that has acquired one or ore genes by artificial means rather than by traditional breeding methods
5. e DNA made from two or more different organisms in vitro

5 Multiple Choice Questions

1. radioactively labeled nucleic acid molecule used to tag a particular DNA sequence
   1. genomic library
   2. nucleic acid probe
   3. Vaccine
   4. forensic science
2. Technique used to screen a single sample for a vast range of different nucleotide sequences stimultaneously; it is often used to study gene expression
   1. genomic library
   2. DNA microarray
   3. DNA fingerprint
   4. DNA technology
3. A set of thousands of DNA segments from a genome, each carried by a plasmid, phage, or other cloning vector.
   1. genomics
   2. genetic marker
   3. gene therapy
   4. genomic library
4. an effort to map the huan genome in total detail by determining the entire nucleotide sequance of human DNA
   1. transgenic organism
   2. DNA fingerprint
   3. Human genome project
   4. telomeres
5. A researcher isolates all the mRNA transcribed from genes in a particular type of cell. This collection of mRNAs is mixed with reverse transcriptase to produce a mixture of cDNAs, each one complementary to one of the mRNAs. The cDNAs are produced in the presence of nucleotides that have benn modified to fluoresce. A small amount of the fluorescently labeled cDNA mixture is added to each of the DNA fragments in the microarray. If a molecule in the cDNA mixture is complementary to a DNA fragment at a particular location on the grid, the cDNA molecule binds to it, becoming fixed there. after nonbinding cDNA is rinsed away, the remaining cDNA produces a detectable glow in the microaray. The pattern of glowing spots inables the researcher to quickly determine which genes are turned on or off in the starting cels
   1. DNA microarray
   2. how microarrays work
   3. genetic marker
   4. genomic library

5 True/False Questions

1. genetic marker → A set of thousands of DNA segments from a genome, each carried by a plasmid, phage, or other cloning vector.

   True        False

2. biotechnology → methods used to study and/or manipulate DNA, including recombinant DNA technology

   True        False

3. Polymerase chain reaction → a polymerase that catalyzes the formation of DNA using RNA as a template

   True        False

4. gel electrophoresis → Technique that uses a gel as a molecular sieve to sparate nucleic acids or proteins on the basis of size or electrical charge

   True        False

5. Comparing whole sets of genes, especially among different organisms, is called → human insuling, human growth hormone, cancer drugs, growht factor for burn treatment

   True        False