Path: Special Stains

5 Written Questions

1. Potassium permanganate on a slide stained with congo red
2. PTAH, black
3. Endogenous, heme, antemortem jaundice
4. Sudan Red (red), Oil Red O (red), and Osmic Acid (black), vacuoles sharply demarcated
5. PAS (Periodic Acid Schiff)- stains pink

5 Multiple Choice Questions

1. Masson-Trichrome
   1. Connective Tissue
   2. Lipids
   3. Glucose
   4. Hemosiderin
2. Von Kossa (black) or Alizarin red (red), deposits usually granular and deeply basophilic
   1. Glucose
   2. Anthracosis
   3. Calcium Deposits
   4. Lipids
3. Black, not stained, deposit in lungs of smokers
   1. Anthracosis
   2. Bilirubin
   3. Fibrin
   4. Glucose
4. Lugol's iodide enchanced with sulfuric acid
   Alone: brown-black
   W/ sulfuric acid: blue-black
   1. Amyloid-general
   2. Amyloid- Gross Staining
   3. Amyloid- microscopic/histologic
   4. Melanin
5. Masson-Fontana (black with white background and red nuclei), PM pseudomelanosis is greenish-black
   1. Lipids
   2. Melanin
   3. Bilirubin
   4. Fibrin

4 True/False Questions

1. Glucose → Sudan Red (red), Oil Red O (red), and Osmic Acid (black), vacuoles sharply demarcated

   True        False

2. Lipofuscin (endogenous) → No Stain, brown/granular, associated with wear and tear especially in permanent cells, autoflorescent and acid-fast antemortem

   True        False

3. Amyloid- microscopic/histologic → Lugol's iodide enchanced with sulfuric acid
   Alone: brown-black
   W/ sulfuric acid: blue-black

   True        False

4. Hemosiderin → Prussian blue (blue-black), want to differentiate between hemosiderin and acid hematin using neutral buffered formalin (no acid hematin), seen inside MO in lungs "heart failure cells"

   True        False