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masteringmicrobiology

In microbiology lab, you make three different culture plates: one general purpose plate, one selective media plate, and one differential media plate. Unfortunately, you forget to label the different plates, so you do not know which is which. To determine the identity of each plate, you inoculate it with two different strains of laboratory bacteria. Which of the following results would indicate the plate you used was the general purpose media?

Both organisms would grow, but they would be dyed different colors.
Only one organism would grow, and it would grow a different color.
Only one organism would grow.
Both organisms would grow equally well.

Both organisms would grow equally well.

In microbiology lab, you make three different culture plates: one general purpose plate, one selective media plate, and one differential media plate. Unfortunately, you forget to label the different plates, so you do not know which is which. To determine the identity of each plate, you inoculate it with two different strains of laboratory bacteria. After inoculation, you see that a particular plate has allowed both organisms to grow, but one has changed color. Which plate do you think this is?
It is impossible to distinguish between selective and differential media in this case.
general purpose media
selective media
differential media

differential media

Which of the following types of culture media would best be used by a veterinarian to grow any bacteria in a blood sample?
selective media
enrichment media
selective and differential media
general purpose media

enrichment media

Which of the following would you expect to be a function of MacConkey agar? Mark all that apply.
allow for the growth of all organisms in a sample regardless of nutritional requirements
allow for the growth of all organisms in a sample that require only the most common nutrients
inhibit the growth of certain bacteria
change the color of some organisms based on their metabolic characteristics

inhibit the growth of certain bacteria
change the color of some organisms based on their metabolic characteristics

Bacterial growth is defined as

increase in bacterial cell population.

In which phase is the rate of cell death equal to the rate of cell growth?

Stationary phase

Why is cell growth typically graphed logarithmically?

Cell growth is rapid, and plotting the log of the number of cells versus the generation on a logarithmic graph produces a linear graph.

Why might cells begin to die at a faster rate than new cells are made?

Lack of nutrients and an increase in cellular waste products.

Which statement about bacterial growth is true?
All bacteria have a generation time of 20 minutes.
When the log of the number of cells is plotted versus the generation, the graph is linear.
Log phase of growth represents the highest total number of living and dead cells.
There are only three phases of growth: lag, logarithmic, and stationary.

When the log of the number of cells is plotted versus the generation, the graph is linear.

What results when a single bacterium reproduces?

Two genetically identical daughter cells

If you begin with six cells, how many cells would you have after three rounds of division?

Forty-eight cells

Which of the following is NOT a step in bacterial cell division?
Replication of the genetic material
Disappearance of nuclear envelope
Splitting apart of two new daughter cells
Cell elongation

Disappearance of nuclear envelope

Which step of binary fission is the reason for genetically identical daughter cells?

Replication of the bacterial chromosome

What enables the copied chromosomes to separate during binary fission?

The chromosomes are attached to different parts of cell membrane, which elongates and thus separates the chromosomes.

How long does it take for the daughter cells from one round of replication to replicate themselves?

No time is required -- they are ready to divide immediately after DNA replication and separation of the daughter cells is complete if conditions are right.

Put the following steps of bacterial replication in the correct order, starting from a parent cell.
1. Cell elongation
2. Septum formation
3. Chromosome replication
4. Separation of daughter cells

3, 1, 2, 4

What would happen if the septum did not form during binary fission?

The parent cell would now have two copies of the chromosome.

Starting with three cells, how many cells would result from three rounds of replication?

Twenty-four

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