What is the use of the KOH test? What is the indicator of a positive KOH?
determine if gram - or +
mixture of KOH and bacteria becomes viscous with gram -
How does the KOH test work?
dissolves gram neg. cell wall
What is the catalase test?
H2O2 test for the production of catalase
Which bacteria do NOT produce catalase?
What active chemical does the motility medium contain?
What color does neutral red turn in the presence of acid?
What color does neutral red turn in the presence of base?
What color does phenol red turn in the presence of acid?
What color does phenol red turn in the presence of base?
What color does bromthymol blue turn in the presence of acid?
What color does bromthymol blue turn in the presence of base?
Which three agars use phenol red?
triple sugar iron agar
mannitol salt agar
Which media is non-selective? (5)
What are 5 examples of selective media?
xylose lysine deoxycholate
What is enrichment media used for?
Blood agar is __________ and ____________ but not ___________.
enriched and differential
T/F blood agar is commonly used for fastidious organisms.
What is A-hemolysis? Color of agar after?
partial destruction of RBC's around bacterial colonies (green color)
What is B-hemolysis? Color of agar after?
complete destruction of RBC's around bacterial colonies (clear color)
What is G-hemolysis? Color of agar after?
No hemolysis (red)
What agar has blood added to it when it is hot to cause hemolysis of the RBC's?
What is the use of chocolate agar?
provide X and V factors for hemophilus spp. and the like
What is the use of mannitol salt agar?
isolation of staph. spp.
What pH indicator is used in mannitol salt agar?
What is the purpose of the MacConkey agar?
isolation and id of enteric gram - bacteria
What chemical in MacConkey agar makes it selective?
What color do lactose fermenters produce on a MacConkey agar? non-fermenters?
What kind of media is Eosin-Methylene blue agar? What is its purpose?
isolation of gram -
What color do coliforms produce on E-M blue agar? Salmonella/shigella?
What is the fxn of xylose lysine deoxycholate agar?
isolation of salmonella and shigella
What spp. grows red on XLD agar?
Which spp. causes XLD to first turn yellow b/c of fermentation of xylose and then red w/ the fermentation of lysine?
What is the urease broth used for?
detection of bacteria's ability to produce urease
What pH indicator is used in urease broth? What color does it turn if the bacteria possesses urease? Why?
red, b/c urea is hydrolized by urease into ammonia which is basic
What is the TSI agar used for?
differentiation of gram - bacteria
What is the pH indicator for TSI agar?
What four properties does the TSI test for?
Ability to ferment glucose and/or lactose and/or sucrose, sulfide production, and gas production
If the only part of a TSI that is yellow is the butt, then you can assume that the organism can _________.
only ferment glucose
If the entire TSI agar is yellow then you can assume the organism can __________.
ferment glucose; sucrose and/or lactose
What is the purpose of the Lysine Iron Agar?
detect the ability of enteric bacteria to decarboxylate lysine
What color with the LIA agar turn if the organism can decarboxylate lysine?
What color will the LIA agar turn if the organism can deaminate lysine?
What is the use of Simmons Citrate agar?
determine the ability of an organism to use citrate as the sole source of carbon and energy
What color with the Simmons Citrate agar turn if the organism can utilize citrate?
deep prussian blue
What is the oxidation-fermentation test for?
to determine if a organism utilizes glucose by fermentation or oxidation
What is the pH indicator in oxidation-fermentation test?
What three properties does the idole medium test for?
motility, ability to utilize ornithine/indole
What color does a + indole test illicit?
What color does a + ornithine test illicit?
What chemical does the oxidase test, test for?
What is the purpose of the oxidase test?
differentiation between pseudomonadaceae and enterobacteriaceae
Enterobacteriaceae are oxidase? Pseudomonas?
neg (no color change)
pos (purple color)
What is the purpose of the micro id test?
differentiation between enterobacteriaceae
What is an antibiogram?
the susceptibility/resistance pattern of bacterial colonies on an agar
What is the fxn of the mueller-hinton agar?
kirby-bauer antimicrobial suscept. test
What does elisa stand for?
enzyme linked immunosorbent assay
What two Ig's does ELISA test for? When are they found?
IgM primary immune responses
IgG secondary immune responses
What is the "gold standard" for Ehrlichia diagnostics?
Where does the ELISA test for Ab's in FLV? IFA test? What is the specific Ab's name?
ELISA-- blood serum
IFA -- infected WBC's
What is the prozone effect?
excess Ab's inhibits agglutination
What is the purpose of the tube agglutination test?
diagnose bacteria and assess immune response to a vaccine.
What chemical is used to degrade tissue and mucous to isolate possible fungal infxns?
What is the standard mycological stain?
lactophenol cotton blue
What is the apperance of a fungus that has been gram stained?
This specific mycological stain, ____________, is used to demonstrate the large capsule of the fungal organism ___________ ___________.
What is the agar of choice for the cultivation of fungi?
Sabouraud Dextrose Agar
What organism are we testing for with the Dermatophyte test medium? what is the pH indicator used?