Lab Final Review
About this set
Created by:
rjones6045 on August 10, 2010
Subjects:
Log in to favorite or report as inappropriate.
Order by
69 terms
Terms | Definitions |
|---|---|
 What is the use of the KOH test? What is the indicator of a positive KOH? | determine if gram - or + mixture of KOH and bacteria becomes viscous with gram - |
| How does the KOH test work? | dissolves gram neg. cell wall |
| What is the catalase test? | H2O2 test for the production of catalase |
| Which bacteria do NOT produce catalase? | obligate anaerobes |
| What active chemical does the motility medium contain? | tetrazolium chloride |
| What color does neutral red turn in the presence of acid? | red |
| What color does neutral red turn in the presence of base? | yellow |
| What color does phenol red turn in the presence of acid? | yellow |
| What color does phenol red turn in the presence of base? | red |
| What color does bromthymol blue turn in the presence of acid? | yellow |
| What color does bromthymol blue turn in the presence of base? | blue |
| What pH indicator is used in MacConkey agar? | neutral red |
| Which three agars use phenol red? | triple sugar iron agar mannitol salt agar XLD |
| Which media is non-selective? (5) | blood trypticase soy thioglycollate mueller-hinton sabouraud dextrose |
| What are 5 examples of selective media? | MacConkey Eosin-methylene blue mannitol salt xylose lysine deoxycholate lowenstein-jensen |
| What is enrichment media used for? | fastidious organisms |
| Blood agar is __________ and ____________ but not ___________. | enriched and differential NOT selective |
| T/F blood agar is commonly used for fastidious organisms. | T |
| What is A-hemolysis? Color of agar after? | partial destruction of RBC's around bacterial colonies (green color) |
| What is B-hemolysis? Color of agar after? | complete destruction of RBC's around bacterial colonies (clear color) |
| What is G-hemolysis? Color of agar after? | No hemolysis (red) |
| What agar has blood added to it when it is hot to cause hemolysis of the RBC's? | chocolate agar |
| What is the use of chocolate agar? | provide X and V factors for hemophilus spp. and the like |
| What is the use of mannitol salt agar? | isolation of staph. spp. |
| What pH indicator is used in mannitol salt agar? | phenol red |
| What is the purpose of the MacConkey agar? | isolation and id of enteric gram - bacteria |
| What chemical in MacConkey agar makes it selective? | crystal violet |
| What pH indicator is used in MacConkey agar? | neutral red |
| What color do lactose fermenters produce on a MacConkey agar? non-fermenters? | brick-red colorless |
| What kind of media is Eosin-Methylene blue agar? What is its purpose? | differential isolation of gram - |
| What color do coliforms produce on E-M blue agar? Salmonella/shigella? | brown-black, amber-colorless |
| What is the fxn of xylose lysine deoxycholate agar? | isolation of salmonella and shigella |
| What spp. grows red on XLD agar? | shigella |
| Which spp. causes XLD to first turn yellow b/c of fermentation of xylose and then red w/ the fermentation of lysine? | salmonella |
| What is the urease broth used for? | detection of bacteria's ability to produce urease |
| What pH indicator is used in urease broth? What color does it turn if the bacteria possesses urease? Why? | phenol red red, b/c urea is hydrolized by urease into ammonia which is basic |
| What is the TSI agar used for? | differentiation of gram - bacteria |
| What is the pH indicator for TSI agar? | phenol red |
| What four properties does the TSI test for? | Ability to ferment glucose and/or lactose and/or sucrose, sulfide production, and gas production |
| If the only part of a TSI that is yellow is the butt, then you can assume that the organism can _________. | only ferment glucose |
| If the entire TSI agar is yellow then you can assume the organism can __________. | ferment glucose; sucrose and/or lactose |
| What is the purpose of the Lysine Iron Agar? | detect the ability of enteric bacteria to decarboxylate lysine |
| What color with the LIA agar turn if the organism can decarboxylate lysine? | purple |
| What color will the LIA agar turn if the organism can deaminate lysine? | red |
| What is the use of Simmons Citrate agar? | determine the ability of an organism to use citrate as the sole source of carbon and energy |
| What color with the Simmons Citrate agar turn if the organism can utilize citrate? | deep prussian blue |
| What is the oxidation-fermentation test for? | to determine if a organism utilizes glucose by fermentation or oxidation |
| What is the pH indicator in oxidation-fermentation test? | bromthymol blue |
| What three properties does the idole medium test for? | motility, ability to utilize ornithine/indole |
| What color does a + indole test illicit? | pink/red |
| What color does a + ornithine test illicit? | purple |
| What chemical does the oxidase test, test for? | cytochrome oxidase |
| What is the purpose of the oxidase test? | differentiation between pseudomonadaceae and enterobacteriaceae |
| Enterobacteriaceae are oxidase? Pseudomonas? | neg (no color change) pos (purple color) |
| What is the purpose of the micro id test? | differentiation between enterobacteriaceae |
| What is an antibiogram? | the susceptibility/resistance pattern of bacterial colonies on an agar |
| What is the fxn of the mueller-hinton agar? | kirby-bauer antimicrobial suscept. test |
| What does elisa stand for? | enzyme linked immunosorbent assay |
| What two Ig's does ELISA test for? When are they found? | IgM primary immune responses IgG secondary immune responses |
| What is the "gold standard" for Ehrlichia diagnostics? | IFA |
| Where does the ELISA test for Ab's in FLV? IFA test? What is the specific Ab's name? | ELISA-- blood serum IFA -- infected WBC's p27 |
| What is the prozone effect? | excess Ab's inhibits agglutination |
| What is the purpose of the tube agglutination test? | diagnose bacteria and assess immune response to a vaccine. |
| What chemical is used to degrade tissue and mucous to isolate possible fungal infxns? | 10-20% KOH |
| What is the standard mycological stain? | lactophenol cotton blue |
| What is the apperance of a fungus that has been gram stained? | + |
| This specific mycological stain, ____________, is used to demonstrate the large capsule of the fungal organism ___________ ___________. | india ink Cryptococcus neoformans |
| What is the agar of choice for the cultivation of fungi? | Sabouraud Dextrose Agar |
| What organism are we testing for with the Dermatophyte test medium? what is the pH indicator used? | Trichophyton equinum phenol red |
First Time Here?
Welcome to Quizlet, a fun, free place to study. Try these flashcards, find others to study, or make your own.