Ch 8 MM

27 terms by dls1540 

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What does cDNA stand for?

Complementary DNA

Which of the following would be a useful phenotypic marker on a vector?
a. Reverse transcriptase
b. Antibiotic resistance gene
c. Restriction enzyme
d. Ligase

b. Antibiotic resistance gene

A plasmid unexpectedly converts to a linear form inside a cell. What will happen to it?

It will be degraded by cellular enzymes

What enzyme is used to make specific cuts in DNA molecules?

Restriction enzymes

What information is conveyed by the name of a restriction enzyme?

The strain of the bacterium in which it was discovered, the species of the bacterium in which it was discovered, and the order of its discovery compared to other restriction enzymes from that bacterium

The small size of a genetic vector is an advantage because __________.

Larger molecules would be too fragile to manipulate in the lab

How do restriction enzymes cut DNA sequences?

They cut DNA at sites, called recognition sites, that have specific nucleotide sequences.

In general, how might recombinant DNA technology be used to prevent a genetic disorder caused by a mutation in a single gene?

To insert a desirable gene, remove an undesirable gene, or replace a defective gene with a functioning gene

What attaches the target gene to a desired location?

DNA ligase

Why would a recombinant DNA molecule be inserted into a host cell?

It can be copied, transcribed, and translated into a desired protein

What is a Southern blot used for?

To locate DNA sequences of interest in a collection of DNA fragments

What is electroporation used for?

Inserting DNA into cells

Protoplasts are valuable in techniques that are used to insert DNA in cells because __________.

They have no cell wall to interfere with the entry of DNA into the cell

What is the function of the primers in PCR?

They provide a 3' end for the DNA polymerase

In which direction does DNA polymerase synthesize the new DNA strand?

5' to 3'

What provides the energy for DNA polymerization in a PCR reaction?

Deoxyribonucleoside triphosphates

Why is DNA polymerase from Thermus aquaticus ideal for PCR?

It can withstand the high temperatures associated with PCR

What is the end goal of PCR?

To quickly increase the number of copies of a specific DNA sequence

Application uses PCR Y/N?
sequencing a gene

Yes

Application uses PCR Y/N?
Diagnosing a disease

Yes

Application uses PCR Y/N?
Providing enough DNA for cloning into another organism

Yes

If a scientist wanted to view the location of a particular species of bacterium within a biofilm, which technique could be used?

fluorescent in situ hybridization (FISH)

Which of the following is NOT involved in the process of DNA fingerprinting?
a. using PCR to make multiple copies of a DNA sample
b. addition of fluorescent nucleotides to a DNA sample
c. gel electrophoresis of DNA fragments
d. cutting a DNA sample with restriction enzymes

b. addition of fluorescent nucleotides to a DNA sample

Four different fluorescent dyes are used in automated DNA sequencing because __________.

there are four different bases in DNA

CC: Keesha's treatment uses polymerase chain reaction (PCR). PCR is used in recombinant DNA technology to ________________.

synthesize millions of copies of a DNA molecule

CC: The restriction enzyme EcoRI is used in recombinant DNA technology to _______________.

cut DNA at a specific palindromic sequence of N-bases.

CC: Vectors are used in recombinant DNA technology to ____________.

deliver a helpful gene into a recipient cell

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