Advertisement Upgrade to remove ads

plasmid

A small circular piece of DNA

transformation

altering or inserting a gene to change an organism;s traits

E. coli

What type of bacteria did we use?

4 agar plates

How many agar plates were used?

araC, GFP, bla, ori

What are the four genes present in the pGLO plasmid?

agar plates with LB broth

What type of medium did we grow E. coli on?

insert pGLO into E.coli to make it glow

What was the transformation we did in the experiment?

araC gene

protein that breaks down arabinose

GFP gene

green fluorescent protein

green fluorescent protein

WHat does GFP stand for?

bla gene

Beta lactamase protein that causes resistance to ampicillin

make the E.coli DNA more accepting of pGLO plasmid

Adding calcium chloride did what to the E.coli?

heat shock

rapid changes in temperature that cause the pores in bacteria membrane to better accept plasmid

ice, water bath, ice

What are the steps of heat shock?

arabinose

on and off switch for GFP gene

expressed

If arabinose is present, GFP is expressed or not expressed?

+pGLO, LB/amo/ara

WHich agar plate glowed?

-pGLO, LB, amp

Which agar plate had no E.coli growing?

-pGLO, LB

What was the control agar plate to make sure bacteria was growing?

-pGLO, LB, amp

What was the control group to make sure ampicillin was present?

jellyfish Aequorea victoria

What is the real-life source of the GFP gene

bacteria but no glow

WHat do you expect to occur in the agar plate with +pGLO, LB, amp?

bacteria and glow

WHat do you expect to occur in the agar plate with +pGLO, LB, amp, ara?

nothing

WHat do you expect to occur in the agar plate with -pGLO, LB, amp?

bacteria, no glow

WHat do you expect to occur in the agar plate with -pGLO, LB?

Please allow access to your computer’s microphone to use Voice Recording.

Having trouble? Click here for help.

We can’t access your microphone!

Click the icon above to update your browser permissions above and try again

Example:

Reload the page to try again!

Reload

Press Cmd-0 to reset your zoom

Press Ctrl-0 to reset your zoom

It looks like your browser might be zoomed in or out. Your browser needs to be zoomed to a normal size to record audio.

Please upgrade Flash or install Chrome
to use Voice Recording.

For more help, see our troubleshooting page.

Your microphone is muted

For help fixing this issue, see this FAQ.

Star this term

You can study starred terms together

NEW! Voice Recording

Create Set