Microbiology Lab Test 1, 4-3 MSA Plate

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7 things needed to know for the MSA plate: name

MSA plate, Mannitol Salt agar

7 things needed to know for the MSA plate: appearance

red, color of biohazard bags

7 things needed to know for the MSA plate: ingredients

pH indicator = phenol red
mannitol = carbohydrate
7.5 % NaCl

7 things needed to know for the MSA plate: purpose

grow and identify Staph aureus

7 things needed to know for the MSA plate: Selective

selective for gram (+) organisms
salt (NaCl) inhibits nonhalophiles
inhibits non staph organisms, gram (-) organisms, and nonhalophiles

7 things needed to know for the MSA plate: differential

pH indicator = phenol red
carbohydrate = mannitol

7 things needed to know for the MSA plate: results

yellow growth = halophile ferments mannitol (S aureus)
red/pink = staph other than staph aureus
poor/no growth = nonhalophile, non staph, gram (-)

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