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Chapter 10 quiz was changed a little so it could work on quizlet

Biotechnology

The use of an organism's biochemical processes to create a product is

Genetic engineering

The various techniques by which scientists manipulate DNA in the lab are called

Restriction endonucleases

DNA strands can be clipped crosswise at selected positions by using enzymes called

Restriction endonucleases

EcoRI and HINDIII are

Palindromes

Sequences of DNA that are identical when read from 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are

Larger fragments move slowly and remain closer to the wells; DNA has an overall negative charge and moves to the positive pole; DNA fragments are stained to see them; An electric current through the gel causes DNA fragments to migrate.

Analysis of DNA fragments in gel electrophoresis involves

Enzymes, fluorescent dyes, and radioisotopes

Gene probes can be labeled for detection with

Included Taq polymerases and Vent Polymerases

DNA polymerases used in PCR

Proteins

The western Blot technique detects

Synthetic DNA oligonucleotides

The primers in PCR are

Cloning host takes up a plasmid

Which step involves transformation?

Pseudomonas fluorescens

Recombinant stains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects:

Can be engineered to become factories for manufacturing proteins; Are often obtained from germ line engineering; Will pass the genes on to their offspring; Commonly includes mice

Transgenic animals

Gene therapy

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called

DNA fingerprinting

This is often used in forensic science to distinquish one sequence of DNA from another by comparing the sequence of the strands at specific loci:

Has a slow growth rate

All of the following are desirable features in a microbial cloning host except it

Human growth hormone, Factor VIII, insulin, and tissue plasminogen activator

Which of the following drugs is produced by genetic engineering and approved for human use?

Mitochondrial DNA

Which of the following is less subject to degradation than in chromosomal DNA and is used as an evolutionary time clock?

Forensics, evolutionary studies, gene mapping,and medicine

The polymerase chain reaction (PCR) has been used in all of the following fields

It cannot be used on specimens older than 5 years

All of the following are correct about DNA fingerprinting except

endonucleases True

Restriction endonucleases are obtained from various species of bacteria.

RNA True

Some genes in bacteria are repressed by antisense RNA that binds to them.

DNA True

When DNA is heated, the two strands will separate.

DNA False

After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.

gene therapy False

An example of gene therapy is the insertion of the gene for human growth hormone into E. coli cells.

cDNA True

Reverse transcription is used to make cDNA from an RNA template.

transfection True

Transformation and transfection are methods used to introduce DNA into host cells.

gene False

Sequencing a gene automatically identifies which gene it is.

normal gene False

The process of introducing a needed, normal gene, into human cells is called DNA mapping.

gene true

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

VNTR True

VNTR's and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting

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