what is the purpose of Eosin Methylene Blue (EMB)
To test for coliforms in environmental samples. coliforms produce acid and gas from lactose fermentation . It inhibits the growth of gram positive organisms
what are the nutrients/food for bacteria for EMB
Peptone,sucrose and lactose
what are the inhibitors of EMB
EOSIN Y Methylene Blue
what are the indocators of EMB
Eosin Y Methylene Blue
what are the growth reactions for EMB
what are the other components of EMB
distilled water and dipotassium phosphate
what is the purpose of Hektoen Enteric Agar(HE)
to isolate and differentiate salmonella and shegella
what are the nutrients or food for bacteria for HE?
what are the inhibitors of HE?
what are the indicators of HE?
bromthymol blue and acid fuschin dyes
what are the growth reactions and growth results of HE?
what are the other components of HE?
sodium thiosulfate, ferric amonium citrate
what is the purpose of the MacConkey agar
to isolate and differentiate enterobacteria based on the ability to ferment lactose and inhibit gram positive bacteria
what are the nurtients or food for bacteria for Macconkey?
beef extract, peptone and lactose, bile salts, neural red and crystal violet
what are the inhibitors of MacConkey agar?
bile salts and crystal violet
what are the indicators of the MacConkey agar?
what are the growth reactions of MacConkey agar?
what are the other components of MacConkey?
pancreatic digest of gelatin and distilled or deionized water
what is the purpose of mannitol salt agar
for isolation and differentiation of Staphylococcus aureus. differentiates pathogenic for nonpathogenic staph
what are the nutrients or food for bacteria for Mannitol salt agar?
beef extract, peptone, agar and distilled or deionized water
what is the inhibitor of Mannitol salt agar?
what is the indicator of Mannitol salt agar
what are the reactions and growth results of mannitol salt agar
what is the purpose of blood agar
allows differentiation of fastidious bacteria based on their ability to hemolyze RBCs
what are the nurients of blood agar
tryptic soy agar and sheep's blood
what are the indicators of blood agar
alpha, beta and gamma hemolysis
what are the reactions and growth results of blood agar
what is the purpose of urea agar?
to determine the spped at which urea is hyrolysized
what are the nutrients of urea extract?
peptone glucose and yeast exract
what is the inhibitor of the urea agar?
what is the indicator of urea agar?
what is the purpose of the triple sugar iron agar (TSI)
differentiate bacteria on the basis of glucose fermentation, lactose fermentation, sucrose fermentation and sulfur reduction
what are the nutrients of TSI?
glucose,lactose,sucrose and sodium thiosulfate
what are the indicator of TSI?
PHENOL RED AND IRON
REACTIONS GROWTH RESULTS
What are the other components of the TSI agar?
animal proteins, ferrous sulfate and sodium thiosulfate
what is the purpose of the catalase test?
differentiates bacteria based on their ability to respire. respiration is converting glucose to ATP. It also tests directly or indirectly for prescence of electron transport chain which generates the ATP of a cell. It differentiates staph(catalase +) from strep(catalase -.) The catalase breaks hydrogen peroxide into water and oxygen. The O2 indicates bubbles on the slide.
what are the indicators
hydrogen peroxide which will form bubbles
what are the reactions or growth results of the catalase test?
If bubbles occur that catalase is present and the symbol is positive. If no bubbles are present than catalase is absent and the symbol is negative.
what is the purpose of the coagulase test?
to differentiate staphylococcus aureus from other gram-positive cocci.( staph aureus uses plasma components in host via coagulase to convert fibrinogen into fibrin protective barriers.)
what is the indicator of the coagulase test?
fibrinogen causing cells to clump
what are the reactions or growth results of the coagulase test?
table 5-32 and table 5-33
what 2 forms does the coagulase enzyme occur in
bound coagulase and free coagulase
what is bound coagulase?
the clumping factor. it's attached to the bacterial cell wall and reacts directly with fibrinogen in plasma
what is free coagulase?
an extracellular enzyme that reacts with a plasma component called coagulase-reacting factor (CRF)
what are the components of blood agar?
tryptic soy agar and sheep blood
designation of an organism based on its ability to grow in the presence of oxygen
bacteria that use oxygen in metabolism
bacteria that don't use oxygen in metabolism
organisms that must have oxygen to grow
a microorganism capable of both fermentation and respiration that grows in the presence and absence of oxygen
anaerobe that grows in the presence of oxygen but doesn't use it metabollically
an organism that requires oxygen but can't tolerate it at atmospheric levels of it
an organism that is destroyed by oxygen
microorganism that requires elevated CO2 levels
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