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what is the purpose of Eosin Methylene Blue (EMB)

To test for coliforms in environmental samples. coliforms produce acid and gas from lactose fermentation . It inhibits the growth of gram positive organisms

what are the nutrients/food for bacteria for EMB

Peptone,sucrose and lactose

what are the inhibitors of EMB

EOSIN Y Methylene Blue

what are the indocators of EMB

Eosin Y Methylene Blue

what are the growth reactions for EMB

TABLE 4-6

what are the other components of EMB

distilled water and dipotassium phosphate

what is the purpose of Hektoen Enteric Agar(HE)

to isolate and differentiate salmonella and shegella

what are the nutrients or food for bacteria for HE?

lactose,sucrose,salicin

what are the inhibitors of HE?

bile salts

what are the indicators of HE?

bromthymol blue and acid fuschin dyes

what are the growth reactions and growth results of HE?

table 4-7

what are the other components of HE?

sodium thiosulfate, ferric amonium citrate

what is the purpose of the MacConkey agar

to isolate and differentiate enterobacteria based on the ability to ferment lactose and inhibit gram positive bacteria

what are the nurtients or food for bacteria for Macconkey?

beef extract, peptone and lactose, bile salts, neural red and crystal violet

what are the inhibitors of MacConkey agar?

bile salts and crystal violet

what are the indicators of the MacConkey agar?

neutral red

what are the growth reactions of MacConkey agar?

table 4-5

what are the other components of MacConkey?

pancreatic digest of gelatin and distilled or deionized water

what is the purpose of mannitol salt agar

for isolation and differentiation of Staphylococcus aureus. differentiates pathogenic for nonpathogenic staph

what are the nutrients or food for bacteria for Mannitol salt agar?

beef extract, peptone, agar and distilled or deionized water

what is the inhibitor of Mannitol salt agar?

NaCl

what is the indicator of Mannitol salt agar

phenol red

what are the reactions and growth results of mannitol salt agar

fig. 4.4

what is the purpose of blood agar

allows differentiation of fastidious bacteria based on their ability to hemolyze RBCs

what are the nurients of blood agar

tryptic soy agar and sheep's blood

what are the indicators of blood agar

alpha, beta and gamma hemolysis

what are the reactions and growth results of blood agar

table 5-30

what is the purpose of urea agar?

to determine the spped at which urea is hyrolysized

what are the nutrients of urea extract?

peptone glucose and yeast exract

what is the inhibitor of the urea agar?

potassium phosphate

what is the indicator of urea agar?

phenol red

what is the purpose of the triple sugar iron agar (TSI)

differentiate bacteria on the basis of glucose fermentation, lactose fermentation, sucrose fermentation and sulfur reduction

what are the nutrients of TSI?

glucose,lactose,sucrose and sodium thiosulfate

what are the indicator of TSI?

PHENOL RED AND IRON

REACTIONS GROWTH RESULTS

TABLE 5-24

What are the other components of the TSI agar?

animal proteins, ferrous sulfate and sodium thiosulfate

what is the purpose of the catalase test?

differentiates bacteria based on their ability to respire. respiration is converting glucose to ATP. It also tests directly or indirectly for prescence of electron transport chain which generates the ATP of a cell. It differentiates staph(catalase +) from strep(catalase -.) The catalase breaks hydrogen peroxide into water and oxygen. The O2 indicates bubbles on the slide.

what are the indicators

hydrogen peroxide which will form bubbles

what are the reactions or growth results of the catalase test?

If bubbles occur that catalase is present and the symbol is positive. If no bubbles are present than catalase is absent and the symbol is negative.

what is the purpose of the coagulase test?

to differentiate staphylococcus aureus from other gram-positive cocci.( staph aureus uses plasma components in host via coagulase to convert fibrinogen into fibrin protective barriers.)

what is the indicator of the coagulase test?

fibrinogen causing cells to clump

what are the reactions or growth results of the coagulase test?

table 5-32 and table 5-33

what 2 forms does the coagulase enzyme occur in

bound coagulase and free coagulase

what is bound coagulase?

the clumping factor. it's attached to the bacterial cell wall and reacts directly with fibrinogen in plasma

what is free coagulase?

an extracellular enzyme that reacts with a plasma component called coagulase-reacting factor (CRF)

what are the components of blood agar?

tryptic soy agar and sheep blood

designation of an organism based on its ability to grow in the presence of oxygen

aerotolerance

bacteria that use oxygen in metabolism

aerobic

bacteria that don't use oxygen in metabolism

anaerobic

organisms that must have oxygen to grow

obligate aerobes

a microorganism capable of both fermentation and respiration that grows in the presence and absence of oxygen

facultative anaerobe

anaerobe that grows in the presence of oxygen but doesn't use it metabollically

aerotolerant anerobes

an organism that requires oxygen but can't tolerate it at atmospheric levels of it

microaerophiles

an organism that is destroyed by oxygen

obligate anaerobes

microorganism that requires elevated CO2 levels

capnophiles

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