Lab Final Flow Chart Gram Positive Cocci

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Lab Final Flow Chart Gram Positive Cocci

Enterococcus faecalis

cocci
pairs/short chains
Bile esculin positive
6.5% NaCl positive
Strep D Enterococci
**Only cocci gram positive to be Bile esculin positive.

Lactococcus lactis

cocci
in milk

Micrococcus luteus

cocci
pairs or tetrads
microaerophilic
Coagulase negative
Catalase positive
Motility negative
Negative stain

Sarcina bicolor

cocci
sarcina
indole negative
MR negative
VP negative
TSI K/K
Nitrate Negative
MacConkey negative
LDC Negative
ODC Negative
(Only difference between this and Sporosarcina ueae is Nitrate test) Sporosarcina is Nitrate positive.

Sporosarcina ureae

cocci
Indole negative
MR Negative
VP Negative
TSI K/K
Nitrate positive
MacConkey negative
LDC Negative
ODC Negative
(Only difference between this and Sarcina bicolor is Nitrate test) Sarcina is Nitrate negative.

Staphylococcus aureus

cocci
grape-like cluster
faculative anaerboe
Coagulase Positive
Mannitol Salt Positive
Catalase Positive
6.5 NaCl positive
Motility Negative
Main diff between Staph aureus and Staph epidermis main is coagulase and manitol salt.
Diff between Staph aureus and Strep bovis is Catalase and 6.5 NaCl. bovis is negative for both.

Staphylococcus epidermis

cocci
grape like cluster
Coagulase Negative
Mannitol Salt Negative
Catalase Positive-weaker
Main diff between Staph aureus and Staph epidermis is coagulase and manitol salt. They are positive for both.

Streptococcus bovis

cocci
pairs or chains
Catalase Negative
6.5% NaCl negative
Motility Negative
Negative Stain
Nonenterococci
alpha, gama hemolysis
Diff between Staph aureus and Strep bovis is Catalase and 6.5 NaCl.
Diff between Strep bovis and Strep pyogenes is hemolysis. pyogenes is beta.

Streptococcus pyogenes

cocci
pairs or chains
faculatively anaerobic
Catalase Negative
Motility Negative
Negative Stain
Strep A
beta hemolysis
Diff between Strep bovis and Strep pyogenes is hemolysis. bovis is alpha, gama.
Diff between pyogenes and Staph aureus/epidermis is Catalase. They are Catalase positive.

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