What components are necessary for DNA replication?
An origin, enzymes, deoxyribonucleotide triphosphates.
Where does replication begin?
At the origin; Dna A- a protein will bind to the origin. Replication begins at the origin and moves out from there in two directions to produce replication forks.
Binds to 3'OH and extends primer by attaching deoxynucleotide triphosphate to the 3'OH end. Will continue to extend this until it collides with the 5' end of a DNA fragment and falls off the replication complex so that Pol I can replace it.
Synthesizes short RNA molecules that serve as the primer for pol III. Initiates switch-back synthesis when it recognizes a small RNA primer complementary to a region of the single-stranded DNA region.
Elongates DNA fragment, while nibbling away the RNA primer; removes RNA and replaces it with DNA- a chromosome must only contain DNA when replication is finished. When all RNA is replaced with DNA Pol I falls of leaving behind a nick.
Closes nick formed by pol I- the protein that joins two DNA fragments together.