1.
After being digested with a restriction enzyme, genomic DNA fragments are separated by gel electrophoresis. Specific fragments can then be identified through the use of a: C) The cell would create incomplete plasmids.
2.
cuts DNA molecules at specific locations: A. restriction enzyme
3.
DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. The purpose of transferring the DNA from a gel to a nitrocellulose paper is to: A) to attach the DNA fragments to a permanent substrate
4.
is used to make complementary DNA (cDNA) from RNA: E) reverse transcriptase
5.
Probes are short, single-stranded DNA or RNA segments that are used to identify DNA fragments with a particular sequence. In order to identify a specific restriction fragment using a probe, what must be done?: E) A, B, and C
6.
produces multiple identical copies of a gene for basic research or for large-scale production of a gene product: B) gene cloning
7.
Restriction fragments of DNA are typically separated from one another by which process?: C) gel electrophoresis
8.
RFLP analysis can be used to distinguish between alleles based on differences in: A) restriction enzyme recognition sites between the alleles
9.
seals the sticky ends of restriction fragments to make recombinant DNA: C) DNA ligase
10.
separates molecules by movement due to size and electrical charge: D) gel electrophoresis
11.
The polymerase chain reaction (PCR) has been used to amplify DNA from which of the following?: The polymerase chain reaction (PCR) has been used to amplify DNA from which of the following?
12.
The segment of DNA shown in the figure below has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?: B
13.
Which of the following best describes the complete sequence of steps occurring during every cycle of PCR?: A) 2, 1, 4
14.
Which of the following is least related to the others?: C) denaturation
15.
Which of the following is least related to the others?: C) DNA microarray assays
16.
Which of the following modifications is least likely to alter the rate at which a DNA fragment moves through a gel during electrophoresis?: A) altering the nucleotide sequence of the DNA fragment
17.
Which of the following procedures would produce RFLPs?: C) incubating DNA with restriction enzymes
18.
Which of the following types of genomes have been sequenced?: d. B and C only
19.
Which of these is not one of the procedures used in Southern blotting?: B) DNA microarray assay
20.
Yeast artificial chromosomes contain which of the following elements?: E) centromere, telomeres, and an origin of replication
