Early Embryogenesis and Maternal Recognition of Pregnancy

55 terms by Daniellebelith

Create a new folder

Advertisement Upgrade to remove ads

zygote

becomes an embryo

embryo

organism in early stage of development

fetus

potential offspring within uterus

conceptus

product of conception and includes
embryo during the early embryonic stage
embryo and extra-embryonic membranes during the pre-implantation stage
fetus and placenta during the post-attachment stage

fertilization four steps

1. development within the zona pellucida
2. hatching of the blastocyst from the zona pellucida
3. maternal recognition of pregnancy
4. extraembryonic membranes and formation

Fertilized Oocyte

Male and female pronuclei within the cytoplasm characterize a developmental stage of the newly fertilized oocyte

ootid

when male and female pronuclei are observed
Largest single cell in the body
undergoes mitotic division called cleavage
first cleave division generates a two-celled embryo, the cells are called blastomere

Blastomere

each has the potential to develop into separate healthy offspring
Blastomeres from the 2-, 4-, and 8- celled totipotent

Totipotency

is a term used to describe a single cell (blastomere) gives rise to a complete, fully formed individual

Mitotic division of Blastomeres

1. occurs simultaneously
2. no net increase in size
3. take place inside zona pellucida

Morula Stage

is characterized by solid balls of cells and indistinguishable blastomeres

Morula stage cells separate into:

two distinct population form
1. inner cells - gap junction allows for intracellular communication. May enable the inner cells to remain in a cluster
2. Outer Cells - tight junctions allow cell to cell adhesion

After tight junctions are formed

fluid begins to accumulate inside the embryo
Fluid filled cavity is called blastocoele

Hatching of the blastocyst

is governed by three forces
1. growth and fluid accumulation
2. production of enzymes by trophoblastic cells
3. contraction of the blastocyst

Blastocyst

embryo with a distinct cavity

Due to tight junctions and gap junctions

the embryo is partitioned into two cell populations.
1. inner cell mass - develops into embryo proper
2. Trophoblastic cells - give rise to the chorion

chorion

the fetal component of the placenta

Blastocyst continues to undergo mitosis,

fluid continues to fill the blastocoele and pressure begins to increase

Concurrent with growth and fluid accumulation

is the production of proteolytic enzymes by the trophoblastic cells
They weaken the zona pellucida so that it ruptures

The blastocyst itself begins to contract and relax

causes intermittent pressure pulses

The zona pellucida ruptures due to

pressure pulses coupled with continued growth and enzymatic degradation

A small crack or fissure in the zona pellucida develops

the cell of the blastocyst squeeze out
The blastocyst now becomes a free-floating embryo within the lumen of the uterus

Extra-embryonic Membranes

after hatching, the conceptus undergoes massive growth
due largely to development of a set of membranes - extra embryonic membranes

Pre-attachment period

Mammalian embryos are subdivided into two primary groups
Most domestic animals and Primates

Most Domestic Animals

preattachment period within the uterus is long (several weeks)
extensive extra embryonic membranes form by a folding process to generate the amnion, chorion, and allanto-chorion

Primates

Preattachement period is short
Blastocyst implant very soon it enters the uterus
Extraembryonic membranes form after implantation

Extraembryonic membranes of the preattached embryo consists of:

yolk sac
chorion
amnion
allantois

Set of 4 anatomically distinctive membranes which originate from

trophoblast
mesoderm
primitive endoderm
embryo

Specific membranes give rise to the following:

yolk sac
chorion
amnion
as the hatched blastocyst grow it develops an additional layer beneath but in contact with the inner cell mass

yolk sac

develops from the primitive endoderm

chorion

eventually attaches to the uterus

amnion

provide a fluid filled protective sac

Primitive Endoderm

Inside lining of the trophoblast
It also forms an evagination at the ventral
portion of the inner cell mass - yolk sac

yolk sac

regress as conceptus develops

chorion

as the blastocyst continues to expand it forms a double membrane
pushes upward in the dorsalateral region of the conceptus and begins to surround it
send wing like projectiong above embryo the aminon begins to form

amnion

complete sac around the embryo

when the chorion fuses over the dorsal portion

of the embryo, it then forms a complete sac around the embryo which constitutes the amnion

Allantois

during the same time the amnion is developing a small evagination from the posterior region of the primitive gut begins to form and is reffered to as allantois

Functions of the Allantois

1. fluid-filled sac that collects liquid waste
2. expands with embryo growth
3. fuses with chorion

Chorioallantoic membrane

when the allantois fuses with the chorion

Chorioallantoic Membrane Function

the membrane is the fetal contribution to the placenta and will provide the surface for attachment to the endometrium

Conceptus must provide a

biochemical signal or pregnancy will terminate

progesterone must be maintained at

sufficiently high levels so that embryogenesis and attachment of the developing conceptus to the endometrium can take place

the embryo enters the uterus

between 2 and 5 days after fertilization depending on species

Maternal Recognition of Pregnancy

the critical series of events by which the conceptus initially signals its presence to the dam enables pregnancy to continue. If an adequate signal is not delivered in a timely manner, the dam will experience luteolysis, progesterone concentrations will decline and the pregnancy will be terminated

Maternal recognition of pregnancy must occur prior to luteolysis

CL produces oxytocin which stimulates PGF2a synthesis.
PGF2a levels are dependent on the number of oxytocin receptors.
Pulsatile secretion of PGF2a occurs in response to luteal oxytocin secretion and luteolysis follows

The Production of PGF2a is dependent upon

a threshold number of oxytocin receptors that are synthesized by endometrial cells at a critical time during the estrous cycle.

When these receptors are available in sufficient numbers,

pulsatile secretion of PGF2a occurs in response to luteal oxytocin secretion and luteolysis follows.
Clearly, this mechanism must be prevented if a successful pregnancy is to proceed

In the ewe and cow

the free-floating blastocyst produces specific proteins that prevent luteolysis
Ovine trophoblastic protein
Bovine trophoblastic proteiin
Interferons produced by trophoblastic cells
Referred to as ovine interferon τ (oIFN-τ) and bovine interferon τ (biFN-τ)

oIFN-τ and bIFN-τ

present in uterus 13-21 days after ovulation
bind to endometrium and inhibits oxytocin receptor synthesis by endometrial cells
Binds to apical portion of uterine glands to promote protein synthesis critical to preimplantation embryonic survival

Sow

control of pregnancy recognition: two major differences
conceptus produces estradiol as signal for maternal recognition of pregnancy
PGF2a is rerouted into uterine lumen

sow conceptus produces

estradiol 11-12 days after fertilization
estrogen doesnt inhibit PGF2a but causes it to be secreted in different directions (away from submucosal capillaries and towards the uterine lumen)
Luminal PGF2a has little access to the circulation so cannot cause luteolysis
There must be at least two conceptuses present in each horn for pregnancy to be maintained

(sow) it is believed that estrogen causes

increased receptor production for prolactin in the endometrium
Prolactin promotes exocrine secretion of PGF2a (into the lumen of the uterus)
Production of estrogen also stimulates uterine contractions which causes proper spacing of the conceptuses along the uterine horn

Human

at the time of implantation (day 7-9 after ovulation) the conceptus secretes a hormone called Human Chorionic Gonadotropin (hCG)
acts like LH-inhibits intraovarian luteolysis
precise mechanism is not known

Queen

is an induced ovulator
if mating does not occur, corpora lutea are not formed and post-estrous period of several days exist before another estrus.
if she breeds, a CL forms and the duration is the same as gestation (60 days)
a signal from the conceptus is not needed because corpora lutea are not lysed before pregnancy is established

Please allow access to your computer’s microphone to use Voice Recording.

Having trouble? Click here for help.

We can’t access your microphone!

Click the icon above to update your browser permissions above and try again

Example:

Reload the page to try again!

Reload

Press Cmd-0 to reset your zoom

Press Ctrl-0 to reset your zoom

It looks like your browser might be zoomed in or out. Your browser needs to be zoomed to a normal size to record audio.

Please upgrade Flash or install Chrome
to use Voice Recording.

For more help, see our troubleshooting page.

Your microphone is muted

For help fixing this issue, see this FAQ.

Star this term

You can study starred terms together

NEW! Voice Recording

Create Set