Transient flora(page 1)
colonize the superficial layers of skin- more amenable to remove by routine washing
Universal Precaution #3(page 2)
Wear masks and eye wear when needed- mask alone not sufficient if splashing is likely
Universal Precaution #5(page 2)
discard dirty needles and sharps immediately in sharps container- do not bend, clip, or recap.
Universal Precaution #6(page 2)
clean blood or contaminated spills by 1) putting on gloves 2) wiping up with paper towels 3) washing with soap and water 4) disinfect with a 1:10 solution of bleach for 10 minutes
Brightfield Microscope(page 7)
allows visible light rays to pass directly through th eye without being deflected by intervening filters
Numerical Aperature(page 9)
A mathematical constant that describes the relative efficiency of a lens in bending light rays
A fungi that's a yeast at 37 degrees but has a mold-like appearance at 25 degrees
hyphal strands are often composed of individual cells sperated by cross walls called septums
Coenocytic or non-septate hyphae(page 12)
Fungi that lack cross walls and are a continuing mass of cytoplasm containing multiple nuclei
What kinds of agars can fungi grow on?(page 12)
Potato dextrose, sabourand, or sabourand dextrose- simple in composition with a low PH of 4.5-5.6 which kill bacteria
Sarcodina- amebic dysentery(page 14)
tophozoite: discrete nuclear membrane with some granules, pseudopods, found in the large intestine, isolated in diarrhetic stools
cyst: round with a thick wall- mature cyst has a quadrinucleated chromatoid body that is sausage shaped
Mastigophora- African sleeping sickness(page 14)
trophozoite: crescent shaped, large center nucleus, granular cytoplasm, 1 flagella, infects peripheral blood streams, no cyst
pear-shaped with sucking disc, 2 bilateral nuclei, clear cytoplasm, 4 pairs of flagella, infects the small intestine, found in diarrhetic stool
oval, 2-4 nuclei, cyst wall, axostyle, parabasal body, 4 pairs of flagella within cyst, found in stool
Ciliophora- dysentery(page 15)
oval, kidney shaped nucleus, vacuolated, cilia, infects the large intestine, found in diarrhetic stool
round, thick walls, 1 nucleus, cilia within cyst, found in stool
parasite toxoplasma gondii(page 18)
dangerous for the preggers
caused by cats
Bergey's manual of determinative bacteriology(page 21)
classified over 7,000 bacteria
first published in 1923
Biosafety levels class 1(page 22)
biological agent that is unlikely to cause human disease( bacillus subtilus, lactobacillus sp.)
Biosafety levels class 2(page 22)
can cause human disease and can be a hazard\
unlikely to spread
effective treatment available
(staphylococcus aureus, hepatitis virus)
Biosafety levels class 3(page 22)
Biological agent that can cause severe human disease and present a serious hazard to workers, may spread but there is a treatment available
(mycobacterium tuberculosis, yersinia pestis)
Biosafety levels class 4 (page 22)
Biological agent that causes severe human disease and is a serious hazard to workers
high risk of spreading
no treatment available
(ebola virus, marburg virus)
Simple stains (page 33)
crystal violet- uniform purple stain
methylene blue- uniform blue stain
reveals size, morphology, and arrangement of cells
Differential stains: gram stain (page 33)
gram + cells are purple
gram - cells are red
differentiates between gram + and gram - bacteria, which is typically the first step in identification
Differential stains: acid-fast stain (page 33)
red acid-fast cells and blue non-acid-fast cells
distinguishes the genera mycobacterium and nocardia from other bacteria
differential stains: endospore stain (page 33)
red vegetative cell
highlights the presence of endospores produced by species int he genera bacillus and clostridium
Special stains: negative stain for capsules (page 33)
background is dark with unstained cells
Special stains: flagella stain (page 33)
flagella become visible for counting and identifying location
suggested doctors were spreading cadaverous particles to other patients and said doctors needed to wash their hands. He was harassed and sent to asylum.
Bacteria often get trapped under rings and fingernails. What 2 problems might this pose for the microbiologist?
The first problem is that you might contaminate the culture of which you are preparing if these bacteria get from underneath your fingernails or ring and onto the culture. The second problem is that because bacteria get trapped so easily you can accidentally take bacteria from the lab and spread it, which could be a problem if you are dealing with pathogens.
List two advantages of hand washing over gloves. List two advantages of gloves over hand washing.
Handwashing because you won't build immunity to the gel when needed, handwashing won't kill good bacteria. Gloves are faster and no resident or transient bacteria is transferred from doctor to patient or patient to doctor.
Three agents used for hand hygiene
2% chlorhexidine antiseptic soap
alcohol-based hand gels which usually contain 60-95% ethanol or isopropanol
Know some multi-drug resistant pathogens
Methacillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant Enterococcus (VRE), Mycobacterium tuberculosis
How does the inclusion of a blue light filter change resolution?
A blue filter may be placed over the light source because the short wavelength of blue light provides maximum resolution
Why is immersion oil important on the oil immersion lens?
By reducing the amount of refraction at this point, more of the light passing through the slide will be directed to the very narrow diameter lens of the high-power objective. The more light, the clearer the image.
mathematical constant that describes the relative efficiency of a lens in bending light rays (it is the objective lens not the oculars we are talking about)
Bergey's Manual of Determinative Bacteriology
7000 bacteria species have been taxonomically classified and documented in it
What would a gram positive smear look like that was halted after the decolorization step? Gram negative smear?
Positive would still be purple. Negative would be colorless
List 2 factors that may make gram positive bacteria appear gram negative.
- if you don't leave the crystal violet on long enough
- if you overuse safranin it will appear more red than should
- using too much acetone alcohol to take off some crystal violet and keep some safranin
What factor could make gram negative bacteria appear gram positive?
- if you don't leave the acetone alcohol on long enough to get rid of all the crystal violet
What is a mordant? What reagent acts as the mordant in the gram negative?
something that hold something in place ex-grams iodine
What chemical components of endospores render these unique structures so resistant to heat?
They have a high concentration of calcium and dipicolinic acid
Define positive and negative chemotaxis?
positive- bacteria goes from areas of low concentration to high concentration
negative- bacteria goes from areas of high concentration to low concentration
Proteus sp. are often termed 'swarmers'. What does this term mean?
They go everywhere- extremely motile
What toxic component are produced from oxygen?
superoxide- O2 . -
peroxide- O2 -2
singlet oxygen - 1O2
Anaerobic infections are often facilitated by coexistence with facultative anaerobes. What role do facultative anaerobes play in sustaining anaerobic infections.
obligate anaerobe can't thrive with oxygen and faculitative can grow in either oxygen or no oxygen. if you have both the facultative will take up the oxygen so that the oligate anaerobe can grow now
Given a mixed broth culture of a facultative anaerobe and an obligate aerobe, how would you obtain a pure culture of each based on oxygen requirements?
Take the sample from the broth and add it to both an aerobic media culture and an anaerobic media culture. From the aerobic culture, there should be two different growths of bacteria. Take the first and put it on a split aerobic culture and a split anaerobic culture. Then take the second and put it opposite to the first on both the aerobic and anaerobic culture. Only one of the two bacteria (the anaerobic one) should grow on the anaerobic culture, and you now have two pure cultures of bacteria.
know opposite way too= mixed broth with FA and an O ANA → first round- grow without oxygen and second round grow with oxygen
What would happen if an organism used up all the carbohydrate in a fermentation tube?
Organisms use sugar first for energy but when all the sugar is used up they have to start breaking down proteins. This increases the pH and causes the indicator to turn purple again.
sees if nitrate reduces to nitrite but it can be broken down to other things. if it is pink before zinc that is a + result. if colorless you add zinc. if red-pink it is NEG because nitrate was not reduced to nitrate on its own. if colorless still- nitrate can be reduced to something other than nitrite
ARE affected by hand gels
MRSA, VRE, mycobacterium TB, and various fungi. Lipophilic viruses: herpes, HIV, influenza, respiratory syncytial, vaccinia virus
A fungi that is a yeast at 37 degrees C but assumes a mold-like appearance at 25 degrees C.
Cause infections of the subcutaneous and deeper tissues such as the nervous system, lungs, and genital area. These are more common among the immunocompromised.
Lacks septum and therefore have a continuous mass of cytoplasm containing multiple nuclei.
potato dextrose agar (or Sabourand agar:
or Sabourand Dextrose (Sab-Dex) agar)
Simple in composition but have low PH (4.5-5.6) which inhibit the growth of bacteria.
Oxidation/reduction (O/R) potential
When oxygen dissolves in a medium, organic compounds become more oxidized and the medium exhibits a positive O/R potential. As oxygen is consumed by microorganisms the O/R potential decreases allowing anaerobes to grow.
Three ways to achieve anaerobic growth
Agar deeps: dissolved oxygen has been boiled out
Brewer's thioglycolate medium: Contains the reducing agent thioglycolate and resazurin specifically used for the cultivation of anaerobes.
Anaerobe jar: the most commonly used. the jar must be supplemented by a gas-generating system, such as a Gas-pak, which produces both hydrogen and carbon dioxide upon the addition of water
O-F Test: Oxidative Fermentative
differentiates the fermentation of glucose. Uses the PH indicator bromthymol blue which turns yellow when positive. If the tube inoculated with oil is green but the other is yellow it is oxidative, but if both are yellow it is fermentative.
Methyl Red and Voges-Proskauer (MR-VP)
Most coliforms growing in a MR-VP medium initially convert some glucose to acids. Positive tests are red, negative are yellow
gram negative, non-spore forming bacilli that ferment lactose with acid and gas production. Found in high concentrations in the intestines and indicative of fecal contamination in water samples.
Cytochrome Oxidase Test
Identifies the presence of cytochrome C. Changes to blue or red when positive.