23 microbiology of food

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catheenewyen  on December 12, 2010

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23 microbiology of food

intrinsic factors
food composition.
pH.
presence/availability of water.
oxidation-reduction potential.
physical structure.
presence of antimicrobial substances
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intrinsic factors food composition.
pH.
presence/availability of water.
oxidation-reduction potential.
physical structure.
presence of antimicrobial substances
putrefaction proteolysis and anaerobic breakdown of proteins, producing foul-smelling amine compounds
lower water activity inhibits microbial growth true
water activity is lowered by drying, addition of salt/sugar
microorganisms that prefer high osmotic pressure osmophilic
microorganisms that prefer low water activity xerophilic
increased surface area promotes microbial growth true
outer skin of vegetables and fruits slows microbial growth true
extrinsic factors temperature (lower temp retards microbe growth).
relative humidity (higher levels promote growth).
atmosphere (oxygen promotes growth)
food spoilage caused by growth of microbes in food.
toxins sometimes produced
ergotism toxic condition caused by growth of fungus in grains
aflatoxins carcinogens produced in fungus-infected grains and nut products
fumonisins carcinogens produced in fungus-infected forn
algal toxins contaminate fish and shellfish
removal of microorganisms usually achieved by filtration.
low temperature refridgeration retards but doesnt stop microbe growth. can still cause spoilage
high temperature procedures canning- food heated in special containers but doesnt kill all microbes
pasteurization- kills pathogens and reduces number of spoilage organisms
dehydration eliminates bacterial growth. free-water loss and increase in solute concentration
GRAS chemical agents "generally recognized as sage"
used for surfaces of food handling equipment. does not penetrate foods UV radiation
uses ionizing radiation to extend shelf life. kills microbes in moist foods by producing peroxides from water radappertization
electrically generated electrons, turned on only when needed and does not penetrate food as deeply as radappertization (gamma) electron beams
bacteriocins bactericidal proteins active against related species.
bacteriophages kills microbes and were approved for use on ready to eat meats
two primary types of food-borne diseases food-borne infections and food intoxications
food-borne infection ingestion of microbes, followed by growth, tissue invasion or release of toxins. found in raw foods
examples of food-borne infections salmonellosis: contaminated meats poultry or eggs
listeriosis: affects pregnant women and immunocompromised individuals.
e.coli: diarrhea
food-borne intoxication ingestion of toxins in foods in which microbes have grown. includes staph food poisoning, clostridium, perfringens food poisoning, and bacillus cereus food poisoning
detection of food-borne pathogens must be rapid and sensitive
methods include: culture techniques, immunological techniques, molecular techniques
PulseNet est by centers for disease control.
-uses electrophoresis to determine DNA patters for pathogens.
FoodNet active surveillance network used to follow 9 major food-borne diseases. traces course and cause of infection in days instead of weeks
major fermentations used are lactic, propionic, ethanolic
fermented milk products rely on lactic acid bacteria
cheese production milk>curd>cheese
producing alcohol must-juice from crushed grapes
mashing- hydrolysis of carbs by adding water and heating
wort- clear liquid with fermentable carbs
beers and ales malt- germinted barley grains having activated enzymes
mash- malt after being mixed with water
hops-provide flavor and help in clearing word
bottom yeasts- used in beer
top yeasts- used in ales
lager- aged beer. CO2 added at bottling
spirits begins with sour mash. after fermentation, distilled to concentrate alcohol
bread production involves bakers years under aerobic conditions maximizing CO2 production which leavens bread.
can be spoiled by bacillus
probiotics microbes added to diet to provide health benefits

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