Public Health

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bfaske6492  on January 25, 2011

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lectures 1-6

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Public Health

DNA (deoxyribonucleic acid)
Bases- Adenine, Thymine, Cytosine, Guanine
Function to store genetic material
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DNA (deoxyribonucleic acid) Bases- Adenine, Thymine, Cytosine, Guanine
Function to store genetic material
RNA (ribonucleic acid) Bases- Adenine, Uracil, Cytosine, Guanine
Three Different Varieties--Transfer, Messenger, Ribosomal
rRNA Makes up 60% of ribosomes
mRNA Record information from DNA and carry it to ribosomes
tRNA Delivers amino acids to proteins at the ribosome to extend the chain
Transcription the synthesis of mRNA from a DNA template
translation the forming of a polypeptide
Ribosomes 1.) made of proteins and rRNA
2.) each has a small and large subunit
3.) each has three binding sites for tRNA on its surface
Ribosomes 1.) each has one binding site for mRNA
2.) facilitates codon and anticodon bonding
3.) components of ribosomes are made in the nucleus and exported to the cytoplasm
4 RNA nucleotides ------------are arranged 20 different ways to make 20 different amino acids
triplets Nucleotide bases exist in -------
Triplets of bases ------are the smallest units that can code for an a.a.
64 possible amino acid codes
codons mRNA base triplets are----
codon AUG 1.) codes for amino acid methionine
2.) functions as a start codon
3.) starts translation
cytoplasm gel like substance found in a cell
chloroplasts a green structure in a plant
cell wall a stiff covering that protects plant cells
nucleus control center of the cell
function: to hold the DNA
chromosomes provides direction for cell to follow
endoplasmic reticulum transportation network, "internal delivery system"
mitchondrion produces energy in the cell
vacuole cell storage sac for food, waste, water
cell membrane surrounds cell material
diffusion movement of gases from higher concentrations to lower concentrations
passive transport materials do not need energy to move from higher concentratio to lower to a lower concentration
active transport materials need energy to move from a higher concentration to lower concentration
osmosis water moving from higher concentration to a lower concentration
nucleolus dark spot in the middle of the nucleus that helps make ribosomes
Rough ER has ribosomes attached to it
function: to help make proteins
Smooth ER No ribosomes
function: to make fats or lipids
Golgi Complex "The shippers"
function: packages, modifies, and transports materials to different location inside/outside of the cell
appearance: stack of pancakes
Lysosomes appearance: circular but bigger than ribosomes
"clean up crews"
function: to break down food into particles the rest of the cell can use and to destroy old cells
mitochondria "The Powerhouse"
Function: Energy formation, breaks down food to make ATP
ATP the major fuel for all cell activities that require energy
Purine, pyrimidine, deoxyribose, and phosphate contained in DNA
Chargaff's published data that shows %A=%T and %C = %G
Rosalind Franklin took pictures of DNA in London that showed some sort of helix
Watson and Crick published a model showing the double helix, and also suggested that Chargaff's rule was due to base-pairing
antiparallel two chains of DNA face in opposite directions
DNA Polymerase First enzyme isolated by Kornberg
DNA Helicase unwinds DNA in front of opening replication fork
It uses ATP, makes single-stranded cut, allows one strand to swivel freely around the other
Single Stranded DNA binding protein bind to separated DNA strands, prevent from base-pairing back together
RNA primase DNA polymerase III cannot start a growing chain from scratch; needs a short primer to add to
DNA polymerase adds new nucleotides at free 3' ends of growing chain, uses base-pairing rules to insert complementary nucleotides
DNA ligase seals any gaps where adjacent nucleotides on one strand have not been covalently joined
Leading strand can make one long, continuous piece of DNA
Lagging strand must grow away from unwinding
Okazaki fragments DNA fragments newly synthesized from lagging strand
A site holds tRNA that is carrying the next amino acid to be added
P site holds tRNA that is carrying the growing polypeptide chain
E site where discharged tRNAs leave the ribosome

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