Pyruvate is transported into the mitochondria and converted into acetyl-CoA by pyruvate dehydrogenase complex in a oxidative decarboxylation reaction.
Describe the events after glycolysis and before the citric acid cycle.
Is a complex that acts like it has multiple enzymes stuck together. It exhibits substrate channeling.
Describe pyruvate dehydrogenase
it allows the cell to bring in a reactant and do multiple reactions before it leaves. Intermediates nerve leave the complex. Streamlined process.
Describe substrate channeling
thiamine deficiency prevents oxidation of pyruvate which is most detrimental to the brain. It causes Beriberi disease (loss of neural function).
Discuss thiamine deficiency diseases
citrate synthase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase complex
What are the three irreversible reactions of the citric acid cycle?
Where does the citric acid cycle occur?
The active site of aconitase forms a specific three-point attachment to citrate- causing only one of the formations to occur.
Why does citrate (a symmetric molecule) react asymmetrically?
Organic molecules such as citrate that have no chiral center but are potentially capable of reacting asymmetrically with an asymmetric active site are now called _____ molecules
Gentle purification does suggest substrate channeling
Does substrate channeling occur in the CAC?
It is a hub in metabolism. It is amphibolic (catabolic pathways leading in and anabolic pathways leading out)
Why is the oxidation of acetate so complicated?
one ATP per pyruvate (two total)
How much ATP is produced by the citric acid cycle?