BIO 111 Chapter 5 Prep-U

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From DNA to protein involves which series of events?

transcription, translation

Genetically modified weed-resistant canola plants were cultivated in Canada, making it possible for farmers to apply herbicides freely to kill the weeds but not the canola plants. What went wrong with this scenario?

The weed-resistant canola plants spread by seed to neighboring farms that weren't growing canola. The weed-resistant canola plants grew out of control, because traditional herbicides could not kill them.

Most genes come in alternative forms called:

alleles.

Which of the following is NOT a difficulty that medicine has encountered in its attempts to cure human diseases through gene therapy?

all of the above are difficulties that medicine has encountered in its attempts to cure human diseases through gene therapy.

The ___________________ is a project to decode the three billion base pairs in the human genome.

Human Genome Project

An organism cultured from a single cell of another organism that is genetically identical to the other organism is called:

a clone.

some forms of dwarfism in children can be treated by ___________________ made by recombinant DNA techniques.

human growth hormone

PCR is a common technique used to amplify large portions of specified gene sequences. What does the acronym PCR stand for?

polymerase chain reaction

Genotype is to phenotype as:

recipe is to cookie.

hat is the most common reason why DNA analyses overturn incorrect criminal convictions?

DNA evidence is much more reliable an identifier of an individual than eyewitness accounts.

Scientists have inserted a gene from the bacterium Bacillus thuringiensis into corn plants in order to:

increase the corn's resistance to insects.

Alternate versions of a gene are called _______________. They can code for different ________________ of the same character.

alleles; traits

Considering the principle of complementary base pairing, you would expect an equal ratio of which two nitrogenous bases in a DNA molecule?

cytosine and guanine

When a triplet of bases in the coding sequence of DNA is GCA, the corresponding codon for the mRNA that is transcribed from it is:

CGU.

During transcription, at the point where the DNA strand being copied has an adenine, _______________ is added to the mRNA.

a uracil

Diabetes is to insulin as __________ is to __________.

dwarfism, human growth hormone

If you thought about DNA as the book of life, then:

the bases are the words and the sugars and phosphate groups are the binding.

Which of the following nucleotide bases are present in equal amounts in DNA?

Both a) and c) are correct.

In humans, genes make up ______ of the DNA.

less than 5%

The full set of an individual organism's DNA is called its:

genome.

What is the most common reason that DNA analyses overturn incorrect criminal convictions?

In more than three-quarters of the cases overturned by DNA analysis, inaccurate eyewitness testimony played an important role in the original guilty verdict.

The technique often used in forensics that identifies individuals based on their genetic differences is referred to as:

DNA fingerprinting.

Which of the following is a symptom of vitamin A deficiency?

blindness

Diabetes is a chronic disease in which the body cannot produce ________________, a chemical that allows cells to take up and break down sugar from the blood.

insulin

Which of the following is a possible medicinal use of cloning?

Both a) and b) are possible medicinal uses of cloning

A person's unique DNA is carried within some of the cells and tissues of their body. These include all of the following EXCEPT:

All of the above contain a person's DNA.

The first cloned vertebrate was Dolly, a sheep, in:

1997

The double helix model of DNA suggested by Watson and Crick was exciting to the scientific community because:

it explained how genetic material could be duplicated by means of the complementary strands.

The combination of DNA from two or more sources is called:

recombinant DNA.

Which of the following statements about mutations is FALSE?

Mutations always decrease an organism's fitness.

"Fast flushers" are people who lack:

a functional enzyme that metabolizes alcohol.
Correct

in humans, genes make up ______ of the DNA.

less than 5%

The expression of a gene to form a polypeptide occurs in two major steps. What are these two steps in their correct chronological order?

transcription and then translation

The central dogma of molecular biology states that:

DNA is transcribed into RNA which is translated into protein.

A mutation that involves the addition, subtraction, or substitution of a single base is known as:

a point mutation.

During transcription the DNA sequence of a gene is copied into a RNA molecule by:

RNA polymerase.

During translation, chain elongation continues until:

a stop codon is encountered.

Which is the correct order of steps in the process of genetic engineering?

chop up DNA, amplify it, insert it into bacterial cells, grow colonies, and identify colonies

Which of the following is TRUE about human DNA?

More than 95% of our DNA is non-coding DNA.

n Tay-Sachs disease, individuals inherit genes with a mutation that causes an inability to produce ______________________ in their lysosomes.

a lipid-digesting enzyme

The gene coding for Bt crystals have been genetically engineered into crop plants in order to:

make crops more resistant to insects.

An orthologus gene from yeast was discovered in a fruit fly. Why is it problematic to determine the function of the gene in the fruit fly even when the yeast ortholog is well characterized in protein structure and function?

The multicellular complexity of the fruit fly may require the ortholog to have additional unknown functions

Most genetic diseases result from mutations that cause a gene to produce a non-functioning ______________, which in turn blocks the functioning of a metabolic pathway.

enzyme

An important difference between mRNA and DNA is:

mRNA can move throughout the cell, while DNA stays in the nucleus.

Which of the following is NOT a reasonable fear of genetically modified foods?

All of the above are reasonable fears of genetically modified foods.

If the start sequence (codon) on an mRNA reads "AUG", what does the base sequence read on the initiator tRNA, carrying methionine? Remember that a codon on the mRNA binds to a complementary base sequence (called an anticodon) in a tRNA.

UAC

To start the transcription process, a large molecule, __________________, recognizes a _______________________.

RNA polymerase; promoter site

he human genome is composed of approximately how many base pairs?

3 billion

Is it possible to clone a dinosaur?

Yes, but scientists would have to find the DNA in a fossilized mosquito, hope the mosquito had bitten a dinosaur, and then isolate all of the dinosaur's DNA.

Many human diseases can be traced to mutations within a particular individual's genome. How does a particular mutation in DNA result in a disease?

All of these are ways in which disease can be caused by a mutation in DNA.

A frameshift mutation:

results from an insertion or deletion in a DNA sequence.

Suppose you are a scientist and you have recently discovered a multicellular organism in the center of a meteorite that has fallen to earth. You run an initial chemical screen and find that this organism does not contain DNA. You begin to run more tests looking for a different molecule that has a similar function to DNA in this new type of life. What properties of this new molecule would you expect to find?

All of the above are expected properties of this new molecule.

Which of the following is TRUE about human DNA?

More than 95% of our DNA is non-coding DNA.

During transcription, at the point where the DNA strand being copied has an adenine, _______________ is added to the mRNA.

a uracil

Phylogenetic trees are built using:

a), b), and c) are all correct.

Which of the following molecules is NOT DIRECTLY involved in translation?

reverse transcriptase

o get from a gene to a protein, two processes must occur: transcription, in which ____________________________, and translation, in which ____________________________.

a copy of the gene's sequence of bases is made; that copy is used to direct the production of a protein

How does an organism gain new gene functions?

Duplication of an existing gene followed by mutation and selection.

Mutation rates:

can be affected by environmental factors.

Using traditional cross-breeding techniques, scientists have been able to create featherless chickens. Which of the following has NOT been shown to be a problem with featherless chickens?

eatherless chickens have trouble digesting corn.

Which of the following is NOT true about DNA?

Humans lack DNA in their brain cells.

A woman who was selected to have her entire genome sequenced would discover that she had at least ___ homolog(s) for every chromosomal gene.

1

One of the four nucleotide bases in DNA is replaced by a different base in RNA. Which base is it, and what is it replaced by?

thymine, replaced by uracil

How are open reading frames identified in a genome?

start and stop codons

Which of the following statements regarding lateral gene transfer is INCORRECT?

Lateral gene transfer is rarely mediated by viral infections.

Duplication of an existing gene followed by a deleterious mutations of that copy would produce which of the following choices?

pseudogenes.

The loss of genetic diversity in crop plants can become disastrous. The Irish Potato Famine is an example of this problem. Which of the following is NOT a correct statement about the Irish Potato Famine?

Most of the potatoes were exported out of Ireland.

When a triplet of bases in the coding sequence of DNA is GCA, the corresponding codon for the mRNA that is transcribed from it is:

CGU

DNA is a macromolecule that stores information. Which component of the DNA is the source of this information?

the base

Which of the following choices is NOT a reason why it is possible for so many pseudogenes to accumulate in the human genome?

The sequence similarity to functional genes allows them to evade excision mechanisms.

During eukaryotic translation, mRNA carries genetic information from the ________ to the ________, where amino acids are assembled into proteins.

nucleus; ribosome

A given section of DNA with the sequence AATGGCTAT is transcribed. What is the corresponding sequence on the mRNA transcription?

UUACCGAUA

Which of the following statements about Bt crystals is correct?

they are produced by soil-dwelling bacteria of the species Bacillus thuringiensis.

Transgenic bacteria are bacteria:

that have a sequence of DNA from another organism inserted into them.

During the polymerase chain reaction (PCR), two strands of DNA are separated by:

exposure to heat.

Gene therapy involves:

introducing non-defective genes into the cells of an individual with a genetic disorder.

Transcription is a synonym for:

RNA synthesis.

Genetic markers are often used to predict the likelihood that a person will develop an inherited illness. Using this information, what is a reasonable definition of a genetic marker?

Both a) and c) are reasonable definitions of "genetic marker."

The three nonsense codons that do not code for any amino acid; UAA, UAG, and UGA are also known as:

stop codons.

Which of the following choices is NOT information that can be obtained from a protein's amino acid sequence?

The exact DNA sequence that it was expressed from.

During transcription, at the point where the DNA strand being copied has an adenine, _______________ is added to the _______________.

a uracil; mRNA

The technique in which a nonfunctioning gene is replaced by a functioning gene in somatic cells is called:

gene therapy

Transgenic salmon:

carry a version of the growth hormone gene that functions year-round, rather than primarily in the summer.

The complementary base pairs in a DNA molecule are stabilized by:

hydrogen bonds

Transcription and translation are mediated by:

RNA

Golden rice:

could help the 250,000 children each year who are at risk of blindness due to vitamin A deficiency.

Which of the following choices aims to identify the common features and functions of genes and genomes of different organisms?

comparative genomics

What is "golden rice"?

Golden rice is rice that has had genes from other organisms added to it. These genes produce beta-carotene.

Why would an error in the DNA sequencing process in the form of a base insertion or deletion be highly problematic when attempting to translate the DNA sequence into an amino acid sequence?

it would cause frameshifts in the sequence, changing the translated amino acid sequence.

which of the following statements about transgenic plants is CORRECT?

Transgenic plants contain genes from more than one species.

Why is it said that homology can never be directly observed?

We will never actually get to see the ancestral organism form which genes evolved from.

Ninety-six percent of the base-pair sequences in chimps and humans are the same. This finding indicates that:

humans are more closely related to chimps than to any other species on earth.

Which of the following are always the same in every unit of the DNA molecule?

the sugar and phosphate group

Which of the following statements regarding nonsynonymous substitutions is INCORRECT?

The rate of nonsynonymous substitutions is higher than the rate of synonymous substitutions in protein-coding genes.

What can be concluded about comparing differences in molecular biology between different species?

Genetic similarities demonstrate species relatedness.

Which of the following can be said about a frameshift mutation?

Only a) and b) are correct.

Suppose you are comparing the following pair of amino acid sequences by sequence alignment:
lys-arg-cys-cys-ser-phe-leu
lys-cys-cys-ile-arg-leu

4; 3

Hierarchical sequencing and shotgun sequencing are both techniques used for:

analyzing DNA fragments for alignment.

In higher eukaryotes, which of the following mutagenic processes is most likely to give rise to a new, independently evolving gene?

accidental gene translocation

When the polymerase chain reaction (PCR) is used to amplify segments of DNA, the sample is first heated, then cooled, in cycles. How many cycles of heating and cooling would be necessary to produce sixteen times the original quantity of DNA?

4

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