Foundation in Microbiology chap. 3
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32 terms
Terms | Definitions |
|---|---|
 Inoculation | Introduction of a sample into a container of media |
| Incubation | Under a condition that allows growth |
| Isolation | Seperating from one species to another |
| Liquid media examples | broth, milk, infusion |
| Liquid media | Water-based, do not solidigy at temperature above freezing; float easily |
| Semi-solid media | Contain an amount of solidified agent so that it's thickened but does not have to be firmed up to be solid; can be used to measured motility; localization of reaction |
| Solid media | Firm surface on which colonies of bacteria and fungi can grow |
| Semi-solid media examples | agar, gelatin |
| Solid media examples | agar |
| Solid liquidfiable media | Physical form change in response to temperature change |
| Solid nonliquidfiable media | Start out solid and stay solid; less use; not versatile |
| Nutrient broth | Liquid; beef extract and peptone |
| Nutrient agar | Solid; beef extract, peptone, agar |
| Agar | Complex polysaccharide isolated from red algae; solid at room temperature; liquidfies at boiling; does not resolidify until it cools to 42C; not digestable for most microbes |
| Synthetic media | Pure organic/inorganic compounds; chemically defined |
| Complex/nonsynthetic media | Has at least 1 ingredient that is not chemically definable |
| General purpose media | Grow a broad range of microbes, usually nonsynthetic |
| Enriched media | Complex organic substances such as blood serum, hemoglobin or special growth factors required by fastidious microbes |
| Selective media | Contain one or more agents that inhibit growth of some microbes and encourage growth of the desired microbes |
| Differential media | Allow growth of several types of microbes and displays visible differences among desired and undesired microbes |
| Reducing media | Contain a substance that absorb oxygen or slow penetration of oxygen into medium; used for growing anaerobic bacteria |
| Carbohydrate fermentation media | Contain sugar that can be fermented, converted to acids, and a pH indicator to show the reaction; basis for identifying bacteria and fungi |
| Pure culture | Contain of medium that grow only a single known species or type of microorganisms |
| Axenic culture | Is a pure culture, but it's free of other living things except the one being studied |
| Subculture | Make a second level culture from a well-isolated colony; a small amount of cells are transferred to a separate container and incubated |
| Mixed culture | Contain 2 or more easily differentiated species |
| Contaminated culture | Has unwanted microbes of uncertain identity |
| Magnification | Ability to enlarge objects |
| Rosolving power | Ability to show details |
| Bright-field microscopes | Most widely used; specimen is darker than surrounding field |
| Dark-field microscopes | Brightly illuminated specimens surrounded by dark fields |
| Phase contrast | Transforms subtle changes in light waves passing through the specimen into differences in light intensity; best for observing intracellular structures |
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