Which is of the following is most likely the number of base pairs in a bacterial chromosome?
Which of the following is a true statement concerning prokaryotic chrmosomes?
They are located in the cytosol
Which of the following molecules functions as a proofreader for a newly replicated strand of DNA?
DNA polymerase III
Which of the following methods of DNA repair involves enzymes that recognize and correct nucleotide errors in unmethylated strands of DNA?
Which of the following is not a mechanism of natural genetic transfer and recombination?
Which of the following statements is true?
Conjugation requires a sex pilus extending from the surface of a cell.
Although cells P and Q are totally unrelated, cell Q receives DNA from cell P and incorporates this new DNA into its chromosome. This process is
horizontal gene transfer
A sequence of nucleotides formed during replication of the lagging DNA strand is?
an Okazaki fragment
the trp operon is repressible. This means it usually ________ and is directly controlled by__________.
active/ a repressor
The three steps in RNA transcription are
initiation of transcription, elongation of the RNA transcript, termination of transcription
an operon consists of _______,_________, and _________ and is associated with a regulatory gene.
promoter, operator, a series of genes
In general, _________ operons are inactive until the substrate of their genes polypeptides is present
A daughter DNA molecule is composed of one original strand and one new strand because DNA replication is _________
A gene for antibiotic resistance can move horizontally among bacterial cells by ________, ____________, and ___________.
transformation, transduction, bacterial conjugation
_____________ are nucleotide sequences containing palindromes and genes for proteins that cut DNA strands.
________ _________ is a recombination event that occurs during gamete formation in eukaryotes.
___________ RNA and _______ RNA are antisense; that is they are complementary to another nucleic acid molecule
Short interference, Micro
Which of the following statements is true concerning recombinant DNA technology?
in involves modification of an organism's genome
After scientists exposed cultures of penicillium to agents X,Y, and Z they examined the type and amount of penicillin produced by the altered fungi ti find the one that is most effective. Agents X,Y, and Z were probably
Which of the following is FALSE concerning vectors in recombinant DNA technology?
Vectors must contain genes for self-replication.
which of the following would be most usefull in following a gene expression in a yeast cell?
Which of the following techniques is used regularly in the study of genomics?
Fluorescent nucleotide bases are sequenced
Which application of recombinant DNA technology involves the production of a distinct pattern of DNA fragments on a gel?
T/F.........Restriction enzymes inhibit the movement of DNA
F. .... restriction enzymes cut DNA at specific sites
T/F...... Restriction enzymes act at specific nucleotide sequences within a double stranded DNA molecule
a thermocyler seperates molecules based on their size, shape and electrical charge.
Which of the following statements is true of bacterial plasmids
They are small circular DNA molecules that can replicate autonomously
Semiconservative DNA replication means that
each daughter DNA molecule is composed of one original strand and one new strand
if the codon AAA is changed to AAG, it still codes for the amino acid lysine; this is an example of a
Which of the following is characteristic of prokaryotic genomes but NOT eukaryotic genomes?
Which of the following processes is involved in the "central dogma" of genetics?
transcription and translation
T/F the structure of DNA explains both its ability to encode genetic information and the way in which it is copied during cell reproduction
The _________ of a transfer RNA molecule is complementary to a codon in a messenger RNA molecule.
Which of the following items is not a part of the name of a restriction enzyme?
the Gram reaction of the source bacterium
Put the following steps in the correct order needed to produce a recombinant vector containing a human gene insert
4. grow bacterial cells
If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?
PCR would stop after one cycle
If you started with a single DNA molecule, how many would you have at the end of six PCR cycles?
In gel electrophoresis, DNA molecules move toward the ________ electrode because they have an overall ________ charge
A library of cloned sequences representing the expressed genes of an organism is known as a
If all the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate farthest from the negative electrode?
250 base pairs