Media
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19 terms
Terms | Definitions |
|---|---|
 Urea Broth | Urea Urease hydrolyzes Urea + H2O> alkaline ammonia and CO2 Phenol Red Internal Yellow = <6.8-8.4>=Red |
| Nitrate Broth | Nitrate Reduce nitrate to nitrates or free nitrogen gas + gas, - no gas Differential |
| Tryptic Soy Geletin | Isolate and cultivate of fastidious microorganisms All purpose |
| Carbohydrate broths | Dextrose, lactose or sucrose |
| Tryptone Broth | 1% tryptophan in H2O Tryptophanase hydrolyzes tryptophan to indole Kovak's External + red ring, - not red |
| MR-VP Broth | Dextrose Stains of coliform baceria by methyl red and Voges-Proskauer MR detects fermentation of dextrose VP detects acetonin from dextrose. Methyl red, VP Test + red, - no color + red, - copper Differential |
| Simmons Citrate Agar | Transport and utilize citrate as the sole carbon source and ammonium ion as sole nitrogen source Brom thymol blue + blue, - no growth, no color change Differential |
| Columbia CNA Agar | Colistin and naldixic acid " " restrict growth of gram negative organisms Alpha, beta, gamma Selective for Gram Positive |
| Levine EMB Agar | Eosin 4 and methylene blue Inhibits gram + Green/pink = A Colorless = K Selective and differential |
| Psudomonas Agar F | Magnesium chloride and potassium sulfate Enhances production of florescence pigment and inhibits production of pyocanin All purpose and differencial |
| Pseudomonas Agar P | Magnesium chloride and potassium sulfate Enhances production of pyocanin and inhibits production of florescence pigment All purpose and differencial |
| TSA | Tryptic Soy Agar NONE All purpose, Gram +/- |
| Blood | Blood hemolysis alpha, beta, gamma internal green halo, clear halo, red/no change |
| DNAse | DNA degraded by enzymes Color Fad Clear = DNase Differential |
| Mannitol | Mannitol Utilize mannitol and high salt concentrations, produces acid Phenol red Yellow halo with red background Selective/diffenential |
| TSIA Slants | Ability to produce H2S, ferment glucose, sucrose and lactose phenyol red .1% dextrose 1% sucrose and lactose yellow = A, red = K, black = H2S, Bubbles = CO2 Differential |
| Selentite Broth | Sodium Selenite Selective enrichment for salmonella species. inhibits growth of gram + and some gram - Selective |
| Hektonen | used to isolate and differenciate salmonella and shigella from G- enteric bacilli Bile salts prevents Gram+ growth Acid fuchin brom thymol blue Orange = +, Blue-green to translucent = - Selective differential |
| Molility Medium S | 1% soft agar allows motility but preserves the stab line. 1% TTC facilitates the detection of motility Red line along stab line All purpose |
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