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catalase

substrate-H202

H202

reagent/indicator in catalase test

bubbles, s. epidermis

positive result of catalase test

oxidase

substrate-cytochrome

tetramethyl-p-phenylenediamine dihydrochloride

reagent/indicator in oxidase test

blue/purple, p. putida

positive result of oxidase test

amylase

substrate-starch

iodine

reagent/indicator in amylase test

zone of hydrolysis, B. subtilis

positive result of amylase

cellulase

substrate-cellulose

sheared/degraded/yellow, c. hutchinsonii

positive result of cellulase test

caseinase

substrate-casein

zone of hydrolysis, b. subtilis

positive result of caseinase test

gelatinase

substrate-gelatin

HCl

reagent/indicator in gelatinase

white ppt and zone of clearing, b. subtilis

positive result of gelatinase test

cysteine desulfhydrase

substrate-cysteine

ferric citrate

reagent/indicator in cysteine desulfhydrase test

black ppt, c. freundii

positive result of cysteine desulfhydrase test

trytophanase

substrate-tryptophan

kovacs reagent

reagent/indicator in trytophanase

red layer, E. coli

positive result of tryptophanase (indole test)

citrate-permease

substrate-citrate

bromthymol blue

reagent/indicator in citrate-permease

blue agar, E. aerogenes

positive result of citrate-permease test

nitrate reductase

substrate-nitrate

red color broth, E. coli

positive result of nitrate reductase

nitrite reductase

substrate-nitrite

nitrate reductase

enzyme tested for using the reagent/indicator alpha-napthol acetate and sulfanilic acid

gas bubbles, P. denitrificans

positive result of nitrite reductase

nitrogenase

substrate-dinitrogen (N2)

ability to grow, A. vinelandii

positive result of nitrogenase test

coagulase

substrate-fibrinogen

fibrin clots, S. aureus

positive result of coagulase test

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