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DTH skin tests measures ______ response Delayed Type Hypersensitivity measures the T cell response (T helper 1) Ex. TB testing Lymphocyte Blastogenesis Test (LBT) measures ___ For mitosis/proliferation of lymphocytes (immunosuppression) Lymphocytes Blastogenesis Test (LBT) with mitogens measures If there is immunosupression What is a mitogen? A non specific antigen that causes lymphocyte proliferation. I tis used to measure immunosuppresion Lymphocyte Blastogenesis Test (LBT) with antigens Tests for the presence of memory B and T cells How is cell proliferation measured in a lymphocyte blastogenesis test? Radioactive Thymidine (H3) is picked up during the cultivation of growth phase and the radioactivity can be measured ELISPOT assay is used to measure ____ The presence of cytokines Explain how the ELISPOT assay works Antibody to the cytokine is bound in a well Activated T cells are added Cytokines that are produced are caught in the ab Cytokine specific antibody with color is added to measure the cytokines T cells that have been stimulated (and are proliferating) upregulate CD____ CD25 What are the 3 things measured by flow cytometry 1) Diameter 2) Granulocytes 3) Color